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The map label for this gene is psdht [H]

Identifier: 82701474

GI number: 82701474

Start: 372407

End: 373327

Strand: Reverse

Name: psdht [H]

Synonym: Nmul_A0340

Alternate gene names: 82701474

Gene position: 373327-372407 (Counterclockwise)

Preceding gene: 82701476

Following gene: 82701470

Centisome position: 11.72

GC content: 54.61

Gene sequence:

>921_bases
ATGCCTCTACATATCCAGACACCATTAATTGAATCGCGCCCTTTAAGCGACAGAACCGGACATTCAGTCTGGCTGAAAAT
GGAAATCGCCCAGCCCTCCGGCTCTTTTAAAGCCCGGGGAATCGGGTATGCGTGTGAAGAATATTTCCGGCGCGGGGCAA
AACGCTTCATTTCATCATCTGGCGGAAACGCCGGCCTTGCGGTTGCCTTTGCCGGACGTAATCTGTCCGTGCCGGTGGTT
ATCGTTGTGCCGGAAACCGCGTCGAAGCGCGCCAGGGAACTGATGGCGCGAGAGGGCGCGGAAGTGATCATCCAGGGAAA
ATCTCTCAGTGAGGCGAATGCTTTTGCGCGATCGCTCGTGCAACCTGAGGACGCTTTTCTGCATCCCTTTGATGATCCGC
TCATTTGGACGGGTCATGCCAGCATAATTGATGAAATAGTCGATACAGGGCCAAAACCTGATGTAATCGTCCTGTCCGTC
GGCGGAGGCGGTCTTCTGTGCGGGGTTATAGAGGGACTGAATCGGAATGAGTGGCAGGATGTGCCCATAATCGCCGTCGA
AACCCAAGGCGCGGACTCATTTGCTCAGGCACTCAATGCAGGAGCGCTGGTGGAGTTGCCCAGGATATCAAGCATTGCGA
CGACATTAGGGGCAAGTGCGGTTTGCAGGCAGGCCCTCGAATATTCGAGAAAACATCGCATTGGGAGCGTCGTATTACCG
GACAAGCTCGCCGTTTCTGCGTGCATGCAATTCCTGCGCGATCATGATGCGGTGGTTGAGCCCGCTTGCGGCGTAGCGCT
GGCGGCGGTCTATGAAAGGGTTCCAGCTTTGTCCGGATACAAAAAAGTATTGATTATCGTGTGTGGCGGTGTCAGCACTA
CGGTTGACCAGTTACAGGAATGGTCGGCCGTGATGCAATAA

Upstream 100 bases:

>100_bases
TTCATTACCGCCAATTCTCCAATCAACAAAGTCTTCAGCTTTGGTTTGAAAGACCGTTTATGATGTATTCTCATGGCTCA
GCTCGATCGAGATCGTATAA

Downstream 100 bases:

>100_bases
GGAATTCCGGATGATGAGTCTTGAAGAAGTGAGGCCTCAGGTGGTCAGCGGTGGCAGAGCCAGGCACTTCCAGGACTTTT
ACAGGGGATAGGGAAAATCA

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: oxobutanoate; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPLHIQTPLIESRPLSDRTGHSVWLKMEIAQPSGSFKARGIGYACEEYFRRGAKRFISSSGGNAGLAVAFAGRNLSVPVV
IVVPETASKRARELMAREGAEVIIQGKSLSEANAFARSLVQPEDAFLHPFDDPLIWTGHASIIDEIVDTGPKPDVIVLSV
GGGGLLCGVIEGLNRNEWQDVPIIAVETQGADSFAQALNAGALVELPRISSIATTLGASAVCRQALEYSRKHRIGSVVLP
DKLAVSACMQFLRDHDAVVEPACGVALAAVYERVPALSGYKKVLIIVCGGVSTTVDQLQEWSAVMQ

Sequences:

>Translated_306_residues
MPLHIQTPLIESRPLSDRTGHSVWLKMEIAQPSGSFKARGIGYACEEYFRRGAKRFISSSGGNAGLAVAFAGRNLSVPVV
IVVPETASKRARELMAREGAEVIIQGKSLSEANAFARSLVQPEDAFLHPFDDPLIWTGHASIIDEIVDTGPKPDVIVLSV
GGGGLLCGVIEGLNRNEWQDVPIIAVETQGADSFAQALNAGALVELPRISSIATTLGASAVCRQALEYSRKHRIGSVVLP
DKLAVSACMQFLRDHDAVVEPACGVALAAVYERVPALSGYKKVLIIVCGGVSTTVDQLQEWSAVMQ
>Mature_305_residues
PLHIQTPLIESRPLSDRTGHSVWLKMEIAQPSGSFKARGIGYACEEYFRRGAKRFISSSGGNAGLAVAFAGRNLSVPVVI
VVPETASKRARELMAREGAEVIIQGKSLSEANAFARSLVQPEDAFLHPFDDPLIWTGHASIIDEIVDTGPKPDVIVLSVG
GGGLLCGVIEGLNRNEWQDVPIIAVETQGADSFAQALNAGALVELPRISSIATTLGASAVCRQALEYSRKHRIGSVVLPD
KLAVSACMQFLRDHDAVVEPACGVALAAVYERVPALSGYKKVLIIVCGGVSTTVDQLQEWSAVMQ

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI33469958, Length=309, Percent_Identity=48.8673139158576, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI19923959, Length=313, Percent_Identity=47.2843450479233, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI11345492, Length=210, Percent_Identity=30, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1790207, Length=275, Percent_Identity=30.1818181818182, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71991565, Length=294, Percent_Identity=31.6326530612245, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508781, Length=296, Percent_Identity=27.7027027027027, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17537387, Length=158, Percent_Identity=29.746835443038, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319788, Length=326, Percent_Identity=37.7300613496933, Blast_Score=205, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6322024, Length=196, Percent_Identity=36.2244897959184, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322023, Length=123, Percent_Identity=39.0243902439024, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322631, Length=288, Percent_Identity=27.4305555555556, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320930, Length=307, Percent_Identity=26.0586319218241, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355833, Length=287, Percent_Identity=30.3135888501742, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 32612; Mature: 32481

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHIQTPLIESRPLSDRTGHSVWLKMEIAQPSGSFKARGIGYACEEYFRRGAKRFISSS
CCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GGNAGLAVAFAGRNLSVPVVIVVPETASKRARELMAREGAEVIIQGKSLSEANAFARSLV
CCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHC
QPEDAFLHPFDDPLIWTGHASIIDEIVDTGPKPDVIVLSVGGGGLLCGVIEGLNRNEWQD
CCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCC
VPIIAVETQGADSFAQALNAGALVELPRISSIATTLGASAVCRQALEYSRKHRIGSVVLP
CCEEEEECCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
DKLAVSACMQFLRDHDAVVEPACGVALAAVYERVPALSGYKKVLIIVCGGVSTTVDQLQE
HHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHH
WSAVMQ
HHHHCC
>Mature Secondary Structure 
PLHIQTPLIESRPLSDRTGHSVWLKMEIAQPSGSFKARGIGYACEEYFRRGAKRFISSS
CEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GGNAGLAVAFAGRNLSVPVVIVVPETASKRARELMAREGAEVIIQGKSLSEANAFARSLV
CCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHC
QPEDAFLHPFDDPLIWTGHASIIDEIVDTGPKPDVIVLSVGGGGLLCGVIEGLNRNEWQD
CCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCC
VPIIAVETQGADSFAQALNAGALVELPRISSIATTLGASAVCRQALEYSRKHRIGSVVLP
CCEEEEECCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
DKLAVSACMQFLRDHDAVVEPACGVALAAVYERVPALSGYKKVLIIVCGGVSTTVDQLQE
HHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHH
WSAVMQ
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = oxobutanoate + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]