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The map label for this gene is gatB

Identifier: 82701457

GI number: 82701457

Start: 348204

End: 349694

Strand: Direct

Name: gatB

Synonym: Nmul_A0323

Alternate gene names: 82701457

Gene position: 348204-349694 (Clockwise)

Preceding gene: 82701456

Following gene: 82701458

Centisome position: 10.94

GC content: 54.53

Gene sequence:

>1491_bases
ATGCAATGGGAAACCGTTATCGGTCTTGAGGTTCATGCCCAGCTCTCGACCGAGTCCAAAATATTCTCCGGGGCTTCCAC
CGCTTTTGGCGCCGCGCCCAATGCGGAGGCCTGCGCTGTCGATCTTGCGCTGCCCGGTGTGTTGCCGGTATTGAATCGGG
GCGCGGTGGAGCGTGCAATCAGGTTTGGCCTGGCAGTGGGAGCAAAAATCAATTCGCCTTCCATTTTTGCCCGCAAGAAT
TATTTTTATCCGGATCTGCCCAAGGGGTATCAGATCAGTCAATACGAGTTGCCGGTGGTCGAAGGGGGGCACATCCAGCT
TCAGGTCGTCCAGGATGGGAAAGAGCAGGAAAAAACCGTGCGCCTCGTGCGGGCGCATCTGGAGGAGGATGCAGGCAAGT
CGTTGCACGAGGATTTTCACGGCATGAGCGGTATCGATCTCAATCGCGCCGGCACACCCCTGCTTGAGATCGTTTCCGAA
CCGGATATGCGCAGCAGCGCCGAAGCCGTGGCATATGCAAAGACATTGCATACGCTCGTCCGCTGGATCGGCATCTGCGA
TGGCAACATGCAGGAGGGTTCCTTTCGCTGCGATGCTAACGTGTCTGTGCGTCCCCGGGGGTCGGACAAGCTCGGTACCC
GCTGTGAAATAAAGAACCTCAACTCGTTCCGTTTTCTCGAGAAGGCGATCGATTACGAGGTCAGGCGGCAGGTCGAAATC
CTCGAAGACGGAGGGTCCATACAGCAACAGACGCGCCTCTTCGATCCGGAAAAGGGTGAAACGCGCGCAATGCGAAGCAA
GGAAGACGCCCAGGATTACCGCTATTTTCCAGATCCGGATCTGCTGCCGCTCGAGATATCCGGGAACTGGATCGAGGAAG
TGAAGAAAGGGTTGCCTGAACTGCCCCAACAAATACGGACACGGTTCGAGCATGACTATGGGCTTTCGATGTACGATGCG
CTCTTGCTCACCGGAGACCGTGATATCTCGGAATATTATGAGGCAGTCGTAGGAAAACTTCCTTCCGACCCTAAACTGTG
CGCAAACTGGGTAATGGGAGAGGTATCGGCTTATCTCAATAATGAAGGAAAGTCGTTCGATGTTTGTCCGTTATCTCCTG
CACAGCTTGCGCAACTTCTTCTGCGCATCAAGGATGGAACCATTTCTGGAAAGATCGCCAAGGATGTGTTCAGACAGATG
TGGGCGCAGGCAGGTACGGAGAGCATCTCATGGAGGAATAGCGAGGGAGTGGATGAGGGCCTTGCAGACCGGATTATCGA
TTCACAAGGACTGAGGCAGATATCCGATTCAAGCGCATTGGAGAATTTGGTACATGAAGTGCTGGCCGCCAACCCGAAAT
CGGTAGAGGAATTCAAGGCAGGCAAGGAAAAGGCCTTCAACGCCCTGATGGGCCAAGTGATGAAGGCCGCCAGAGGCAAA
GCGAATCCAGCGCAGGTGAATGAGATATTGAGGAAGAAGCTGACGGAATAG

Upstream 100 bases:

>100_bases
CAAAGGAGTAGAGAGCAGATGGTCAGTTGTGGCGAGCCGCTTAGTTCAAGTCGCTACTCATCCCTCATAAGGCATCCGTA
TCAGAAAAGGGTATTCTAAA

Downstream 100 bases:

>100_bases
GTCATGAGCGGACCGAATGAACCGGGAACGAACCGGAATCTTTATCTTTGCTCTGATAGGAGCTCCTGCCCCAGTTCCAT
AGTCTCCCCGGAAAAAGATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MQWETVIGLEVHAQLSTESKIFSGASTAFGAAPNAEACAVDLALPGVLPVLNRGAVERAIRFGLAVGAKINSPSIFARKN
YFYPDLPKGYQISQYELPVVEGGHIQLQVVQDGKEQEKTVRLVRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSE
PDMRSSAEAVAYAKTLHTLVRWIGICDGNMQEGSFRCDANVSVRPRGSDKLGTRCEIKNLNSFRFLEKAIDYEVRRQVEI
LEDGGSIQQQTRLFDPEKGETRAMRSKEDAQDYRYFPDPDLLPLEISGNWIEEVKKGLPELPQQIRTRFEHDYGLSMYDA
LLLTGDRDISEYYEAVVGKLPSDPKLCANWVMGEVSAYLNNEGKSFDVCPLSPAQLAQLLLRIKDGTISGKIAKDVFRQM
WAQAGTESISWRNSEGVDEGLADRIIDSQGLRQISDSSALENLVHEVLAANPKSVEEFKAGKEKAFNALMGQVMKAARGK
ANPAQVNEILRKKLTE

Sequences:

>Translated_496_residues
MQWETVIGLEVHAQLSTESKIFSGASTAFGAAPNAEACAVDLALPGVLPVLNRGAVERAIRFGLAVGAKINSPSIFARKN
YFYPDLPKGYQISQYELPVVEGGHIQLQVVQDGKEQEKTVRLVRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSE
PDMRSSAEAVAYAKTLHTLVRWIGICDGNMQEGSFRCDANVSVRPRGSDKLGTRCEIKNLNSFRFLEKAIDYEVRRQVEI
LEDGGSIQQQTRLFDPEKGETRAMRSKEDAQDYRYFPDPDLLPLEISGNWIEEVKKGLPELPQQIRTRFEHDYGLSMYDA
LLLTGDRDISEYYEAVVGKLPSDPKLCANWVMGEVSAYLNNEGKSFDVCPLSPAQLAQLLLRIKDGTISGKIAKDVFRQM
WAQAGTESISWRNSEGVDEGLADRIIDSQGLRQISDSSALENLVHEVLAANPKSVEEFKAGKEKAFNALMGQVMKAARGK
ANPAQVNEILRKKLTE
>Mature_496_residues
MQWETVIGLEVHAQLSTESKIFSGASTAFGAAPNAEACAVDLALPGVLPVLNRGAVERAIRFGLAVGAKINSPSIFARKN
YFYPDLPKGYQISQYELPVVEGGHIQLQVVQDGKEQEKTVRLVRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSE
PDMRSSAEAVAYAKTLHTLVRWIGICDGNMQEGSFRCDANVSVRPRGSDKLGTRCEIKNLNSFRFLEKAIDYEVRRQVEI
LEDGGSIQQQTRLFDPEKGETRAMRSKEDAQDYRYFPDPDLLPLEISGNWIEEVKKGLPELPQQIRTRFEHDYGLSMYDA
LLLTGDRDISEYYEAVVGKLPSDPKLCANWVMGEVSAYLNNEGKSFDVCPLSPAQLAQLLLRIKDGTISGKIAKDVFRQM
WAQAGTESISWRNSEGVDEGLADRIIDSQGLRQISDSSALENLVHEVLAANPKSVEEFKAGKEKAFNALMGQVMKAARGK
ANPAQVNEILRKKLTE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=510, Percent_Identity=40, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI115534094, Length=482, Percent_Identity=31.9502074688797, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6319391, Length=509, Percent_Identity=32.0235756385069, Blast_Score=229, Evalue=9e-61,
Organism=Drosophila melanogaster, GI45550808, Length=510, Percent_Identity=36.078431372549, Blast_Score=328, Evalue=6e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_NITMU (Q2YC90)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411023.1
- ProteinModelPortal:   Q2YC90
- SMR:   Q2YC90
- STRING:   Q2YC90
- GeneID:   3784000
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0323
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2YC90
- ProtClustDB:   PRK05477
- BioCyc:   NMUL323848:NMUL_A0323-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54971; Mature: 54971

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWETVIGLEVHAQLSTESKIFSGASTAFGAAPNAEACAVDLALPGVLPVLNRGAVERAI
CCCCEEEEEEEEEECCCHHHHHCCCCHHCCCCCCCCCEEEEHHCCCHHHHHCCHHHHHHH
RFGLAVGAKINSPSIFARKNYFYPDLPKGYQISQYELPVVEGGHIQLQVVQDGKEQEKTV
HHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCEECCEEECCEEEEEEECCCHHHHHHH
RLVRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMRSSAEAVAYAKTLHTLV
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RWIGICDGNMQEGSFRCDANVSVRPRGSDKLGTRCEIKNLNSFRFLEKAIDYEVRRQVEI
HHHHHCCCCCCCCCEEECCCCEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LEDGGSIQQQTRLFDPEKGETRAMRSKEDAQDYRYFPDPDLLPLEISGNWIEEVKKGLPE
HHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCHH
LPQQIRTRFEHDYGLSMYDALLLTGDRDISEYYEAVVGKLPSDPKLCANWVMGEVSAYLN
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
NEGKSFDVCPLSPAQLAQLLLRIKDGTISGKIAKDVFRQMWAQAGTESISWRNSEGVDEG
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
LADRIIDSQGLRQISDSSALENLVHEVLAANPKSVEEFKAGKEKAFNALMGQVMKAARGK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ANPAQVNEILRKKLTE
CCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQWETVIGLEVHAQLSTESKIFSGASTAFGAAPNAEACAVDLALPGVLPVLNRGAVERAI
CCCCEEEEEEEEEECCCHHHHHCCCCHHCCCCCCCCCEEEEHHCCCHHHHHCCHHHHHHH
RFGLAVGAKINSPSIFARKNYFYPDLPKGYQISQYELPVVEGGHIQLQVVQDGKEQEKTV
HHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCEECCEEECCEEEEEEECCCHHHHHHH
RLVRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMRSSAEAVAYAKTLHTLV
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RWIGICDGNMQEGSFRCDANVSVRPRGSDKLGTRCEIKNLNSFRFLEKAIDYEVRRQVEI
HHHHHCCCCCCCCCEEECCCCEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LEDGGSIQQQTRLFDPEKGETRAMRSKEDAQDYRYFPDPDLLPLEISGNWIEEVKKGLPE
HHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCHH
LPQQIRTRFEHDYGLSMYDALLLTGDRDISEYYEAVVGKLPSDPKLCANWVMGEVSAYLN
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
NEGKSFDVCPLSPAQLAQLLLRIKDGTISGKIAKDVFRQMWAQAGTESISWRNSEGVDEG
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
LADRIIDSQGLRQISDSSALENLVHEVLAANPKSVEEFKAGKEKAFNALMGQVMKAARGK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ANPAQVNEILRKKLTE
CCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA