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The map label for this gene is gatA [H]

Identifier: 82701456

GI number: 82701456

Start: 346621

End: 348114

Strand: Direct

Name: gatA [H]

Synonym: Nmul_A0322

Alternate gene names: 82701456

Gene position: 346621-348114 (Clockwise)

Preceding gene: 82701455

Following gene: 82701457

Centisome position: 10.89

GC content: 56.49

Gene sequence:

>1494_bases
TTGCTTCATTTTGCTTCATCCAGCGAAAACCAGCCATCAGCCATGTTGAATGCCAGCCTCAAGCAACTTTCCGCGATGCT
TGCCGCAAGGAAAATTTCCAGCGTTGAGTTGACTAGTGAATTTCTCAAGCGTTCCCGAGCGCTTAATCCCGAATACAACG
CCTTCATCACTCTCGATGAAAGGACGAGCCTCGCGCAGGCGCAGGCTGCGGACATCTTGATCGGATCCGGGCGCGGGCAG
CCCCTGACCGGCGTTCCCATTGCACAAAAGGATATCTTCTGTACCAAGGGCTGGCTCACTACCTGCGGATCGAAAATGCT
GTCGAATTTCATCTCGCCCTATGACGCCCACGTGATCGGGCGCTTCAATGCCGTGGGCGCAGTCAATATTGGTAAAACCA
ATATGGACGAATTCGCCATGGGTTCCAGCAATGAAACCTCCTTTTACGGGCCGGTAAAAAATCCCTGGGATACCGCAGCC
GTGCCAGGGGGAAGCTCCGGCGGTTCCGCCTGCGCGGTGGCAGCACGTATGGCGCCTGCGGCGACCGGGACGGACACCGG
TGGCTCGATCCGGCAACCGGCGGCGTTATGCGGCATTTCAGGCCTCAAGCCGACATACGGGCTGGTATCGCGCTATGGCA
TGATCGCCTTCGCATCCAGCCTCGATCAGGGTGGGCCGATGGCTAAATCAGCGGAAGACCTGGGGCTGATGCTCAACGTC
ATGGCAGGGTTCGATGCCCGCGATTCAACCAGTCTCGAGCGCGAACCGGAAGATTACACACGCGACCTGGAAAAGCCGCT
CGACGGTCTGCGCATTGGCCTGCCAAAGGAATATTTCGTCAAGGAAATAGCCGAGGACGTGGCGCGCGCGGTAGAAGCCG
CCATTGGCCAGTATCGCAAGCTGGGAGCCGAAACGGTGGAGGTGTCGCTGCCTAATACGAAGCTTTCGGTTCCGGTTTAT
TACGTGCTTGCCCCCGCCGAAGCTTCGAGCAACCTGTCGCGCTTTGATGGCGTACGCTACGGTTATCGTGCTCCCGATTA
CCTGGATCTCAACGACATGTATCGGAAAACCCGTGCGCAGGGCTTTGGTTCAGAGGTGAAACGCCGCATCCTGATCGGCA
CTTATGTGCTGTCGCATGGATATTATGACGCGTACTACATCCAGGCGCAGAAGCTGCGCCGCCTGATGGCACAGGATTTC
GCCGAGGCATTCAAGGAGTGCGACATCATCATGGGCCCCACTTCTCCTACAGTGGCATTCGACCTCGGAGAGAGAAGCAG
CGACCCGGTTCAGATGTACTTATCCGATGCCTACACTATTGCGGTTAACCTGGCCGGCCTACCCGCGATGTCCATTCCGG
TTGGCTTCGGGCATAAAAACAGACCGGTTGGATTGCACATCATCGGCAATTATTTTGCCGAGGCGCAAATGCTGAACGTG
GCCCATCAATATCAGCTTGAATCAGATTGGCATACACGAATGCCAAAGGAGTAG

Upstream 100 bases:

>100_bases
CCTAAAGTCATCGAGTAGCGTTATCGAGTAGCGCCACGCAGGATCTCTGCGGACGGCTCCCCTGTAATTTCTATTCTCCT
TCCAGGAGCCTTTTCACGTT

Downstream 100 bases:

>100_bases
AGAGCAGATGGTCAGTTGTGGCGAGCCGCTTAGTTCAAGTCGCTACTCATCCCTCATAAGGCATCCGTATCAGAAAAGGG
TATTCTAAAATGCAATGGGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MLHFASSSENQPSAMLNASLKQLSAMLAARKISSVELTSEFLKRSRALNPEYNAFITLDERTSLAQAQAADILIGSGRGQ
PLTGVPIAQKDIFCTKGWLTTCGSKMLSNFISPYDAHVIGRFNAVGAVNIGKTNMDEFAMGSSNETSFYGPVKNPWDTAA
VPGGSSGGSACAVAARMAPAATGTDTGGSIRQPAALCGISGLKPTYGLVSRYGMIAFASSLDQGGPMAKSAEDLGLMLNV
MAGFDARDSTSLEREPEDYTRDLEKPLDGLRIGLPKEYFVKEIAEDVARAVEAAIGQYRKLGAETVEVSLPNTKLSVPVY
YVLAPAEASSNLSRFDGVRYGYRAPDYLDLNDMYRKTRAQGFGSEVKRRILIGTYVLSHGYYDAYYIQAQKLRRLMAQDF
AEAFKECDIIMGPTSPTVAFDLGERSSDPVQMYLSDAYTIAVNLAGLPAMSIPVGFGHKNRPVGLHIIGNYFAEAQMLNV
AHQYQLESDWHTRMPKE

Sequences:

>Translated_497_residues
MLHFASSSENQPSAMLNASLKQLSAMLAARKISSVELTSEFLKRSRALNPEYNAFITLDERTSLAQAQAADILIGSGRGQ
PLTGVPIAQKDIFCTKGWLTTCGSKMLSNFISPYDAHVIGRFNAVGAVNIGKTNMDEFAMGSSNETSFYGPVKNPWDTAA
VPGGSSGGSACAVAARMAPAATGTDTGGSIRQPAALCGISGLKPTYGLVSRYGMIAFASSLDQGGPMAKSAEDLGLMLNV
MAGFDARDSTSLEREPEDYTRDLEKPLDGLRIGLPKEYFVKEIAEDVARAVEAAIGQYRKLGAETVEVSLPNTKLSVPVY
YVLAPAEASSNLSRFDGVRYGYRAPDYLDLNDMYRKTRAQGFGSEVKRRILIGTYVLSHGYYDAYYIQAQKLRRLMAQDF
AEAFKECDIIMGPTSPTVAFDLGERSSDPVQMYLSDAYTIAVNLAGLPAMSIPVGFGHKNRPVGLHIIGNYFAEAQMLNV
AHQYQLESDWHTRMPKE
>Mature_497_residues
MLHFASSSENQPSAMLNASLKQLSAMLAARKISSVELTSEFLKRSRALNPEYNAFITLDERTSLAQAQAADILIGSGRGQ
PLTGVPIAQKDIFCTKGWLTTCGSKMLSNFISPYDAHVIGRFNAVGAVNIGKTNMDEFAMGSSNETSFYGPVKNPWDTAA
VPGGSSGGSACAVAARMAPAATGTDTGGSIRQPAALCGISGLKPTYGLVSRYGMIAFASSLDQGGPMAKSAEDLGLMLNV
MAGFDARDSTSLEREPEDYTRDLEKPLDGLRIGLPKEYFVKEIAEDVARAVEAAIGQYRKLGAETVEVSLPNTKLSVPVY
YVLAPAEASSNLSRFDGVRYGYRAPDYLDLNDMYRKTRAQGFGSEVKRRILIGTYVLSHGYYDAYYIQAQKLRRLMAQDF
AEAFKECDIIMGPTSPTVAFDLGERSSDPVQMYLSDAYTIAVNLAGLPAMSIPVGFGHKNRPVGLHIIGNYFAEAQMLNV
AHQYQLESDWHTRMPKE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=38.6, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI195972892, Length=495, Percent_Identity=25.2525252525253, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI166795287, Length=218, Percent_Identity=31.1926605504587, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=38.7243735763098, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=23.780487804878, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=324, Percent_Identity=26.5432098765432, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=236, Percent_Identity=27.9661016949153, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=330, Percent_Identity=27.5757575757576, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=330, Percent_Identity=27.5757575757576, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=198, Percent_Identity=26.2626262626263, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=462, Percent_Identity=34.8484848484849, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6319685, Length=494, Percent_Identity=27.3279352226721, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=483, Percent_Identity=24.0165631469979, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=41.4342629482072, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=28.9738430583501, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=28.9738430583501, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=28.9738430583501, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=28.9738430583501, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=27.1287128712871, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24644968, Length=245, Percent_Identity=31.8367346938775, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45550774, Length=516, Percent_Identity=23.6434108527132, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648435, Length=516, Percent_Identity=23.6434108527132, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=516, Percent_Identity=23.6434108527132, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=24.4399185336049, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648441, Length=437, Percent_Identity=23.1121281464531, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=437, Percent_Identity=23.1121281464531, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53863; Mature: 53863

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHFASSSENQPSAMLNASLKQLSAMLAARKISSVELTSEFLKRSRALNPEYNAFITLDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
RTSLAQAQAADILIGSGRGQPLTGVPIAQKDIFCTKGWLTTCGSKMLSNFISPYDAHVIG
CHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEEE
RFNAVGAVNIGKTNMDEFAMGSSNETSFYGPVKNPWDTAAVPGGSSGGSACAVAARMAPA
CCCCEEEEECCCCCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
ATGTDTGGSIRQPAALCGISGLKPTYGLVSRYGMIAFASSLDQGGPMAKSAEDLGLMLNV
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MAGFDARDSTSLEREPEDYTRDLEKPLDGLRIGLPKEYFVKEIAEDVARAVEAAIGQYRK
HHCCCCCCCCCCCCCCHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGAETVEVSLPNTKLSVPVYYVLAPAEASSNLSRFDGVRYGYRAPDYLDLNDMYRKTRAQ
CCCCEEEEECCCCEEECEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
GFGSEVKRRILIGTYVLSHGYYDAYYIQAQKLRRLMAQDFAEAFKECDIIMGPTSPTVAF
CCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEE
DLGERSSDPVQMYLSDAYTIAVNLAGLPAMSIPVGFGHKNRPVGLHIIGNYFAEAQMLNV
ECCCCCCCCCEEEECCCEEEEEEECCCCEEECEECCCCCCCCEEEEEHHHHHHHHHHHHH
AHQYQLESDWHTRMPKE
HHHHCCCCCHHHCCCCC
>Mature Secondary Structure
MLHFASSSENQPSAMLNASLKQLSAMLAARKISSVELTSEFLKRSRALNPEYNAFITLDE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
RTSLAQAQAADILIGSGRGQPLTGVPIAQKDIFCTKGWLTTCGSKMLSNFISPYDAHVIG
CHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEEE
RFNAVGAVNIGKTNMDEFAMGSSNETSFYGPVKNPWDTAAVPGGSSGGSACAVAARMAPA
CCCCEEEEECCCCCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
ATGTDTGGSIRQPAALCGISGLKPTYGLVSRYGMIAFASSLDQGGPMAKSAEDLGLMLNV
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MAGFDARDSTSLEREPEDYTRDLEKPLDGLRIGLPKEYFVKEIAEDVARAVEAAIGQYRK
HHCCCCCCCCCCCCCCHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGAETVEVSLPNTKLSVPVYYVLAPAEASSNLSRFDGVRYGYRAPDYLDLNDMYRKTRAQ
CCCCEEEEECCCCEEECEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHC
GFGSEVKRRILIGTYVLSHGYYDAYYIQAQKLRRLMAQDFAEAFKECDIIMGPTSPTVAF
CCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEE
DLGERSSDPVQMYLSDAYTIAVNLAGLPAMSIPVGFGHKNRPVGLHIIGNYFAEAQMLNV
ECCCCCCCCCEEEECCCEEEEEEECCCCEEECEECCCCCCCCEEEEEHHHHHHHHHHHHH
AHQYQLESDWHTRMPKE
HHHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA