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The map label for this gene is wcaL [C]

Identifier: 82701424

GI number: 82701424

Start: 311145

End: 312257

Strand: Direct

Name: wcaL [C]

Synonym: Nmul_A0290

Alternate gene names: 82701424

Gene position: 311145-312257 (Clockwise)

Preceding gene: 82701423

Following gene: 82701425

Centisome position: 9.77

GC content: 58.04

Gene sequence:

>1113_bases
ATGAAAGCTGGCTTGCGCATTGGATTGGTCGGGCCTCTGCCTCCTCCTTCAGGGGGAATGGCCAACCAGACGTTGCAGTT
GGCAGGACTCCTGCGAGAAGAGCATGTTGAGGTGGAGCTGATTCAGGTCAATCGTCCCTACCGGCCTGGCTGGATTGGAA
GATTCAAGGGGATCAGGGCGGGCTTCCGCCTGCTGTCCTATGTCAGCCAACTGTGGCTTTCCGCCGGCAGAGTTCAATTA
TTTCACGTGATGGCCAATTCCGGCTGGTCGTGGCACTTGTTTGCTGCTCCCGCCATCTGGATTGCACGGCTCAGGGGTAA
GCCGGTGATTATCAATTATCGGGGAGGCGAGGCGGACTCCTTCTTTGATAAAGCCTTTTCGTGGGTAAAGCCGAGCTTGT
CAAGAGCAAATGCAATTATCGTTCCTTCCGGGTTTCTGGAAGGGGTTTTCGGCAAACGCGGCTTTTCGGCCAGTATCGTT
CCCAATATCATCGACCTGAGCCGCTTCGGAGCAGGAATGCGGTCCGATACCTCCACGGTGGAAGCCGGACCTGACTCTCC
CCATATCATCGTCACCCGCAATCTGGAGCCGATTTACGACAATGAGACTGCACTGCGCGCCTTTCACATCGTAAGACGCT
CCTTCCCCGCTGCGAAGCTCACCTTGGCCGGATCCGGCCCGGAGCGGCACACGTTGGAGCAACTTGCCTTCGCATTGGGC
ATAGGGGGGGCCGTGACATTCACCGGGCGCGTGGACAACGAAGGCATGGCTGCAATCTATCGCAGCGCGAGTGTGATGCT
CAATCCGAGCCTCGCCGATAATATGCCTATATCGATACTTGAAGCTTTGGCAAGCGGCGTCCCGGTGGTGAGTACGAACG
TGGGGGGTGTCCCCTATCTCGTTGAGCATGAAAAGAACGCCTTGCTCGTACCCGCGCGCGACCCCCAGGCGATGGCCAAT
GCAGTTCTGCGGCTGCTCAATGATGCAGCCAGGACCCGGCGTCTGATCGAGGCGGGTATGGAATGCGTGCAGCGCTACAC
CTGGCCGAACGTGCGCGTCCCCCTGTTCCGTGTCTATGAGCAGGTTCTTGCCGCACCAGACAGATCCTGTTGA

Upstream 100 bases:

>100_bases
CCGGGGCGCGCGATTTTGTCGAAACCCAGCGCAACTGGGCTGGCAGCGTGGCCGGCTATAAGGAGATCTATCGCACTCTC
CTTTCAAGGAAAGCATCGTC

Downstream 100 bases:

>100_bases
GTGACGCTGAAAGCTTTGATAAATGAAAAACAGGGACTGGTATACATCGCTTGTATCGGGTCTTCTCTTTCCGCTGCAAG
AGCGCCTCAAGGATCACTCC

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKAGLRIGLVGPLPPPSGGMANQTLQLAGLLREEHVEVELIQVNRPYRPGWIGRFKGIRAGFRLLSYVSQLWLSAGRVQL
FHVMANSGWSWHLFAAPAIWIARLRGKPVIINYRGGEADSFFDKAFSWVKPSLSRANAIIVPSGFLEGVFGKRGFSASIV
PNIIDLSRFGAGMRSDTSTVEAGPDSPHIIVTRNLEPIYDNETALRAFHIVRRSFPAAKLTLAGSGPERHTLEQLAFALG
IGGAVTFTGRVDNEGMAAIYRSASVMLNPSLADNMPISILEALASGVPVVSTNVGGVPYLVEHEKNALLVPARDPQAMAN
AVLRLLNDAARTRRLIEAGMECVQRYTWPNVRVPLFRVYEQVLAAPDRSC

Sequences:

>Translated_370_residues
MKAGLRIGLVGPLPPPSGGMANQTLQLAGLLREEHVEVELIQVNRPYRPGWIGRFKGIRAGFRLLSYVSQLWLSAGRVQL
FHVMANSGWSWHLFAAPAIWIARLRGKPVIINYRGGEADSFFDKAFSWVKPSLSRANAIIVPSGFLEGVFGKRGFSASIV
PNIIDLSRFGAGMRSDTSTVEAGPDSPHIIVTRNLEPIYDNETALRAFHIVRRSFPAAKLTLAGSGPERHTLEQLAFALG
IGGAVTFTGRVDNEGMAAIYRSASVMLNPSLADNMPISILEALASGVPVVSTNVGGVPYLVEHEKNALLVPARDPQAMAN
AVLRLLNDAARTRRLIEAGMECVQRYTWPNVRVPLFRVYEQVLAAPDRSC
>Mature_370_residues
MKAGLRIGLVGPLPPPSGGMANQTLQLAGLLREEHVEVELIQVNRPYRPGWIGRFKGIRAGFRLLSYVSQLWLSAGRVQL
FHVMANSGWSWHLFAAPAIWIARLRGKPVIINYRGGEADSFFDKAFSWVKPSLSRANAIIVPSGFLEGVFGKRGFSASIV
PNIIDLSRFGAGMRSDTSTVEAGPDSPHIIVTRNLEPIYDNETALRAFHIVRRSFPAAKLTLAGSGPERHTLEQLAFALG
IGGAVTFTGRVDNEGMAAIYRSASVMLNPSLADNMPISILEALASGVPVVSTNVGGVPYLVEHEKNALLVPARDPQAMAN
AVLRLLNDAARTRRLIEAGMECVQRYTWPNVRVPLFRVYEQVLAAPDRSC

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 40330; Mature: 40330

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAGLRIGLVGPLPPPSGGMANQTLQLAGLLREEHVEVELIQVNRPYRPGWIGRFKGIRA
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHH
GFRLLSYVSQLWLSAGRVQLFHVMANSGWSWHLFAAPAIWIARLRGKPVIINYRGGEADS
HHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHH
FFDKAFSWVKPSLSRANAIIVPSGFLEGVFGKRGFSASIVPNIIDLSRFGAGMRSDTSTV
HHHHHHHHHCCHHHHCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCE
EAGPDSPHIIVTRNLEPIYDNETALRAFHIVRRSFPAAKLTLAGSGPERHTLEQLAFALG
ECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHC
IGGAVTFTGRVDNEGMAAIYRSASVMLNPSLADNMPISILEALASGVPVVSTNVGGVPYL
CCCEEEEEEEECCCCHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEE
VEHEKNALLVPARDPQAMANAVLRLLNDAARTRRLIEAGMECVQRYTWPNVRVPLFRVYE
EECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QVLAAPDRSC
HHHCCCCCCC
>Mature Secondary Structure
MKAGLRIGLVGPLPPPSGGMANQTLQLAGLLREEHVEVELIQVNRPYRPGWIGRFKGIRA
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHH
GFRLLSYVSQLWLSAGRVQLFHVMANSGWSWHLFAAPAIWIARLRGKPVIINYRGGEADS
HHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHH
FFDKAFSWVKPSLSRANAIIVPSGFLEGVFGKRGFSASIVPNIIDLSRFGAGMRSDTSTV
HHHHHHHHHCCHHHHCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCE
EAGPDSPHIIVTRNLEPIYDNETALRAFHIVRRSFPAAKLTLAGSGPERHTLEQLAFALG
ECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHC
IGGAVTFTGRVDNEGMAAIYRSASVMLNPSLADNMPISILEALASGVPVVSTNVGGVPYL
CCCEEEEEEEECCCCHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEE
VEHEKNALLVPARDPQAMANAVLRLLNDAARTRRLIEAGMECVQRYTWPNVRVPLFRVYE
EECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QVLAAPDRSC
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]