Click here to switch to the map view.

The map label for this gene is pimF [H]

Identifier: 82701413

GI number: 82701413

Start: 301733

End: 302683

Strand: Direct

Name: pimF [H]

Synonym: Nmul_A0279

Alternate gene names: 82701413

Gene position: 301733-302683 (Clockwise)

Preceding gene: 82701412

Following gene: 82701414

Centisome position: 9.48

GC content: 51.1

Gene sequence:

>951_bases
ATGCCATTGGAAATCAGCATTGTCTCCACCATGTACCGGTCGCGGCCGTTTCTTGACCGGTTCCTTGCCGAATCCCTGCA
AGCCTTGTCTGCGATCAAGTGTGACCATTTTGAGATATTGCTCGTCAATGATGGCTCGCCGGATGAGTCCCTGGCTTACG
CGCTCGAACGCCGGAGAGACATCCCGCAACTCGTGGTGGTTGATCTTGCGCGGAACTTTGGGCATCACGCTGCTATACAG
GCTGGATTACGGCATGCACGGGGAAATCTCGTTTTTCTCATCGACTGCGACCTGGAGGTGTCGCCGCTGGTGCTGGCAGA
GTTTTATTCCAAACTACGCGAGACCAAATCCGACGTCGTTTTTGGCTATCAGGACGTACGCAAAGGCGGGCGGTTCGAAC
AAATCAGCGGCGGTCTGTTCTGGAAGGGATTCAACCTGCTCAGCGATGTAAAGATTCCTGAAAACATTCTTACGGAACGG
ATCATGACGCGCCGTTACGTGGAAGCGCTACTGCAGATGGGCGACCGGAATCTTTTTCTCGGTGGTATGATGAGCTGGAC
AGGTTTTCAGCAGATCGGCCTGCCTGTCGTCAAGAAACAGCGTGAAGGCAGAAGCACATATACTCTCCTGCGACGTATCG
GATTGATGATCAATGCAGTAAGTTCATTCTCCGCCCAGCCTCTGATCTGGCTGTTCAACATAGGCGTAGTGATTACCCTG
CTGAGTTTCTCATTTGCATTCTATCTTGTATTGCGAAAGCTACTGTTTGACGACACCCTCATGGGTTTCACTTCAGTAAT
GACCATGATGATGCTCAGCCTCGGTATTCTCACCACGGCAATGGGCCTTGTTGGTATCTACCTGGGGAAGGTGTTCAATC
AGGTGCAGAACCGTCCCAATTATATCGTGAGAAATATTTTTACCGCTGATGATCAACCTGCAACCGATTGA

Upstream 100 bases:

>100_bases
CTTCACCTGGAAAAGTAAATTAAATCATCTCCACTTGAAGCTGTAATTAAGTAGAATTTGACCCGGGGCACCAGAAGATG
AAGGAATCGGATCTGATCAT

Downstream 100 bases:

>100_bases
CAGGACAGTAACGCGGCGTCCTTCTGATTTAGAATGAAGAGCAAGGGATAATTAAAAAGCTCGTGCTCCATTTCTGCTTA
AATAAAGGAAGATATTCATG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPLEISIVSTMYRSRPFLDRFLAESLQALSAIKCDHFEILLVNDGSPDESLAYALERRRDIPQLVVVDLARNFGHHAAIQ
AGLRHARGNLVFLIDCDLEVSPLVLAEFYSKLRETKSDVVFGYQDVRKGGRFEQISGGLFWKGFNLLSDVKIPENILTER
IMTRRYVEALLQMGDRNLFLGGMMSWTGFQQIGLPVVKKQREGRSTYTLLRRIGLMINAVSSFSAQPLIWLFNIGVVITL
LSFSFAFYLVLRKLLFDDTLMGFTSVMTMMMLSLGILTTAMGLVGIYLGKVFNQVQNRPNYIVRNIFTADDQPATD

Sequences:

>Translated_316_residues
MPLEISIVSTMYRSRPFLDRFLAESLQALSAIKCDHFEILLVNDGSPDESLAYALERRRDIPQLVVVDLARNFGHHAAIQ
AGLRHARGNLVFLIDCDLEVSPLVLAEFYSKLRETKSDVVFGYQDVRKGGRFEQISGGLFWKGFNLLSDVKIPENILTER
IMTRRYVEALLQMGDRNLFLGGMMSWTGFQQIGLPVVKKQREGRSTYTLLRRIGLMINAVSSFSAQPLIWLFNIGVVITL
LSFSFAFYLVLRKLLFDDTLMGFTSVMTMMMLSLGILTTAMGLVGIYLGKVFNQVQNRPNYIVRNIFTADDQPATD
>Mature_315_residues
PLEISIVSTMYRSRPFLDRFLAESLQALSAIKCDHFEILLVNDGSPDESLAYALERRRDIPQLVVVDLARNFGHHAAIQA
GLRHARGNLVFLIDCDLEVSPLVLAEFYSKLRETKSDVVFGYQDVRKGGRFEQISGGLFWKGFNLLSDVKIPENILTERI
MTRRYVEALLQMGDRNLFLGGMMSWTGFQQIGLPVVKKQREGRSTYTLLRRIGLMINAVSSFSAQPLIWLFNIGVVITLL
SFSFAFYLVLRKLLFDDTLMGFTSVMTMMMLSLGILTTAMGLVGIYLGKVFNQVQNRPNYIVRNIFTADDQPATD

Specific function: May play only a redundant role in maintaining cell wall viability and bacterial virulence [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788692, Length=308, Percent_Identity=29.2207792207792, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788588, Length=307, Percent_Identity=27.3615635179153, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 35783; Mature: 35652

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLEISIVSTMYRSRPFLDRFLAESLQALSAIKCDHFEILLVNDGSPDESLAYALERRRD
CCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCC
IPQLVVVDLARNFGHHAAIQAGLRHARGNLVFLIDCDLEVSPLVLAEFYSKLRETKSDVV
CHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHE
FGYQDVRKGGRFEQISGGLFWKGFNLLSDVKIPENILTERIMTRRYVEALLQMGDRNLFL
ECHHHHHCCCCEEHHCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
GGMMSWTGFQQIGLPVVKKQREGRSTYTLLRRIGLMINAVSSFSAQPLIWLFNIGVVITL
CCHHHHCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSFSFAFYLVLRKLLFDDTLMGFTSVMTMMMLSLGILTTAMGLVGIYLGKVFNQVQNRPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YIVRNIFTADDQPATD
HHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PLEISIVSTMYRSRPFLDRFLAESLQALSAIKCDHFEILLVNDGSPDESLAYALERRRD
CEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCC
IPQLVVVDLARNFGHHAAIQAGLRHARGNLVFLIDCDLEVSPLVLAEFYSKLRETKSDVV
CHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHE
FGYQDVRKGGRFEQISGGLFWKGFNLLSDVKIPENILTERIMTRRYVEALLQMGDRNLFL
ECHHHHHCCCCEEHHCCCEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
GGMMSWTGFQQIGLPVVKKQREGRSTYTLLRRIGLMINAVSSFSAQPLIWLFNIGVVITL
CCHHHHCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSFSFAFYLVLRKLLFDDTLMGFTSVMTMMMLSLGILTTAMGLVGIYLGKVFNQVQNRPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YIVRNIFTADDQPATD
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]