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The map label for this gene is wcaL [C]

Identifier: 82701384

GI number: 82701384

Start: 267714

End: 268838

Strand: Direct

Name: wcaL [C]

Synonym: Nmul_A0250

Alternate gene names: 82701384

Gene position: 267714-268838 (Clockwise)

Preceding gene: 82701383

Following gene: 82701385

Centisome position: 8.41

GC content: 49.96

Gene sequence:

>1125_bases
ATGAAGAAACCCCGCGTTGTCCTGGTTGCCTTGCACTTTGCCGAATATGCTTTGAATTTGGCGCTCGCAGTAGCTGAAAG
TCGAGAGGTGCTCCTTGTTCTGTATCGTGGTAACGCTGATCGCGAACTCGGCCCAGATTGGCTTAAGGGTATCCGTCATC
CGTCCTTGATGCTACTGATACTGGATAGGCCGCGCTCCCCTCTGATGATTCTTGGCAACGCAAGACGGCTTGTACGCGCA
ATCCAGCAATTCAAGCCTGATGTAATTCATTATCAGGAAGATCCTCGCGATGAAGTAATTCTAGGACTGTATTTCCTACG
TTCGATACCCATAGTACTTACCGTACACGATCCCACCCCCCATTCAGGGACAGATTCCAATCGTTTGCGCTATTCGCGCT
TTAGGTTATACCTGCCCCTGATGAGGCGAGCTGCGGATATCGCCATTACCCACGGTGGTTTGCTTGCTGACAATTTGATC
AAAGCGTTTCCTCATTTCAAAGATAAAGTCCAACCCATTCCACACGGTCCGCTAGGGCTTTTCAGCGGTACCCAACCATC
ACATCCCAGCGGGTTCCGCTTGCTGTTTTTCGGACGTATCTTCGAATATAAGGGGCTCAGTTATTTTGTCGACTCAGTCA
TAATGCTTCGAGACAAAGGCTACCCGGTGATTGGGGTAGTTGCGGGCTTCGGTACGGATCTAGCGCGGCATAGACAACGC
ATGGAAGAAGCCGGTTGTTTTGAAATTTTGGATAGATATATTGCCGCGAAAGAAGTCCCCGATCTGTTTCTCGACTCTTT
TGCGGTAGTCCTTCCCTATGTTGATGGCACCCAAAGCGGCGTCGCGGCCATGGCATTAGGCTTTGCTCGACCTGTAGTAG
CCAGCGCTGTGGGATCCATACCTGAACTAGTCAGAAATGGGGAAAACGGATTGCTTGTCCCCCCGTCGGATTCAATCGCT
CTGTCCGAGGCAATCGAAACCCTTCTTAATGATACTCAGCTCTGGGAGCAATTGACCGCAGGAGCTCAGAGGCTAAGAGA
TCAGGAACTTTCTTGGGAATCCATTGCTGACAAAACAATTCGTGTTTATGAATTGGCTGGAAAAATGCGACTGGCCTCAA
CATAG

Upstream 100 bases:

>100_bases
CTTTTTTCCTCTATGTAATCGATAGAGGAGCCAAACAGCGCGACAAAGCCAATGAGCCCGTCCAAGTGCGGGCACATTTT
TTAAGAGCAGGAAACTTCCG

Downstream 100 bases:

>100_bases
TCTACACTGGAATTAGGCTATGCTAAGGGTTAAAGTAGTGAGACATGGTGATGCAGTGATGTCGGGCCGAGCATAAATTG
CTGGCAGTGAAATATGTAGG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKKPRVVLVALHFAEYALNLALAVAESREVLLVLYRGNADRELGPDWLKGIRHPSLMLLILDRPRSPLMILGNARRLVRA
IQQFKPDVIHYQEDPRDEVILGLYFLRSIPIVLTVHDPTPHSGTDSNRLRYSRFRLYLPLMRRAADIAITHGGLLADNLI
KAFPHFKDKVQPIPHGPLGLFSGTQPSHPSGFRLLFFGRIFEYKGLSYFVDSVIMLRDKGYPVIGVVAGFGTDLARHRQR
MEEAGCFEILDRYIAAKEVPDLFLDSFAVVLPYVDGTQSGVAAMALGFARPVVASAVGSIPELVRNGENGLLVPPSDSIA
LSEAIETLLNDTQLWEQLTAGAQRLRDQELSWESIADKTIRVYELAGKMRLAST

Sequences:

>Translated_374_residues
MKKPRVVLVALHFAEYALNLALAVAESREVLLVLYRGNADRELGPDWLKGIRHPSLMLLILDRPRSPLMILGNARRLVRA
IQQFKPDVIHYQEDPRDEVILGLYFLRSIPIVLTVHDPTPHSGTDSNRLRYSRFRLYLPLMRRAADIAITHGGLLADNLI
KAFPHFKDKVQPIPHGPLGLFSGTQPSHPSGFRLLFFGRIFEYKGLSYFVDSVIMLRDKGYPVIGVVAGFGTDLARHRQR
MEEAGCFEILDRYIAAKEVPDLFLDSFAVVLPYVDGTQSGVAAMALGFARPVVASAVGSIPELVRNGENGLLVPPSDSIA
LSEAIETLLNDTQLWEQLTAGAQRLRDQELSWESIADKTIRVYELAGKMRLAST
>Mature_374_residues
MKKPRVVLVALHFAEYALNLALAVAESREVLLVLYRGNADRELGPDWLKGIRHPSLMLLILDRPRSPLMILGNARRLVRA
IQQFKPDVIHYQEDPRDEVILGLYFLRSIPIVLTVHDPTPHSGTDSNRLRYSRFRLYLPLMRRAADIAITHGGLLADNLI
KAFPHFKDKVQPIPHGPLGLFSGTQPSHPSGFRLLFFGRIFEYKGLSYFVDSVIMLRDKGYPVIGVVAGFGTDLARHRQR
MEEAGCFEILDRYIAAKEVPDLFLDSFAVVLPYVDGTQSGVAAMALGFARPVVASAVGSIPELVRNGENGLLVPPSDSIA
LSEAIETLLNDTQLWEQLTAGAQRLRDQELSWESIADKTIRVYELAGKMRLAST

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 41744; Mature: 41744

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPRVVLVALHFAEYALNLALAVAESREVLLVLYRGNADRELGPDWLKGIRHPSLMLLI
CCCCCEEEEEHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCHHHHHHCCCCCEEEEE
LDRPRSPLMILGNARRLVRAIQQFKPDVIHYQEDPRDEVILGLYFLRSIPIVLTVHDPTP
ECCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCC
HSGTDSNRLRYSRFRLYLPLMRRAADIAITHGGLLADNLIKAFPHFKDKVQPIPHGPLGL
CCCCCCCCEEHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCC
FSGTQPSHPSGFRLLFFGRIFEYKGLSYFVDSVIMLRDKGYPVIGVVAGFGTDLARHRQR
CCCCCCCCCCCEEEEEEHHHHHHCCHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHH
MEEAGCFEILDRYIAAKEVPDLFLDSFAVVLPYVDGTQSGVAAMALGFARPVVASAVGSI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PELVRNGENGLLVPPSDSIALSEAIETLLNDTQLWEQLTAGAQRLRDQELSWESIADKTI
HHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHE
RVYELAGKMRLAST
EEEEHHCCEECCCC
>Mature Secondary Structure
MKKPRVVLVALHFAEYALNLALAVAESREVLLVLYRGNADRELGPDWLKGIRHPSLMLLI
CCCCCEEEEEHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCHHHHHHCCCCCEEEEE
LDRPRSPLMILGNARRLVRAIQQFKPDVIHYQEDPRDEVILGLYFLRSIPIVLTVHDPTP
ECCCCCCEEEECCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCC
HSGTDSNRLRYSRFRLYLPLMRRAADIAITHGGLLADNLIKAFPHFKDKVQPIPHGPLGL
CCCCCCCCEEHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCC
FSGTQPSHPSGFRLLFFGRIFEYKGLSYFVDSVIMLRDKGYPVIGVVAGFGTDLARHRQR
CCCCCCCCCCCEEEEEEHHHHHHCCHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHH
MEEAGCFEILDRYIAAKEVPDLFLDSFAVVLPYVDGTQSGVAAMALGFARPVVASAVGSI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PELVRNGENGLLVPPSDSIALSEAIETLLNDTQLWEQLTAGAQRLRDQELSWESIADKTI
HHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHE
RVYELAGKMRLAST
EEEEHHCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]