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The map label for this gene is epsA [H]

Identifier: 82701376

GI number: 82701376

Start: 259367

End: 260161

Strand: Direct

Name: epsA [H]

Synonym: Nmul_A0242

Alternate gene names: 82701376

Gene position: 259367-260161 (Clockwise)

Preceding gene: 82701375

Following gene: 82701377

Centisome position: 8.15

GC content: 53.21

Gene sequence:

>795_bases
ATGTTCCAAATTTTGGTTCGTACCTTGATTCTTTTATCCATGTTGTTTTCGGTTAAGGTTTTTGCCGAGAGTCCTGACTA
CCCGCTGGGTCCGGGTGATATCCTGCGCATTCAGGTTTTTCAAAATCCTGACCTTACTACTGAAACCCGGGTTTCCGAAA
GTGGTTCCATCACCTTTCCGCTGGTTGGCGCTCTCGAGGTCGGAGGACTTTCTGTTGCTTCCGCCGAAAATAAACTTGCA
GCCGCCCTTAAAAAGGGAGGGTTCATAAAGCAACCCCAAGTGACCATTGTCCTCCTGCAGATGAGGGGTAGTCAGGTGAG
TGTGCTGGGGCAGGTCAATCGACCGGGACGCTTTCCACTGGAAACCCTGAGCAGGGTGAGTGACATGCTGGCTGCCGCGG
GAGGTACCACTCCGCTCGGCGACGATTTTGCAATCGTTACCGGTACACGCAACGGACAGGCCTTCCGAAAGGTGATCGAT
ATTCCCGCACTCTATCTGGAAGAGAGATCCGATGAAGATATCATCCTTGCTGGAGGAGACACGATTTATGTTCACCGGGC
TCCCGTTTTCTATATTTACGGCGAGGCCCAGCGGCCTGGTGCTTATCGTATCGAGCGAGGCATGACCGTAATGCAGGCTC
TGGCTCAGGGCGGGGGGCCGACTCCGCGTGGTAGCGAATGGTGGTTGCGCCTTCATCGCAGAAACGCGGATGGGGTCGTG
GAGAAGCTTTCGCCCGACATGACCGATGCTGTCCAGCCTAATGACATAATTTATGTGCGAGAAAGCATTTTTTAA

Upstream 100 bases:

>100_bases
GCTAGGGATTCAAGTTCGCCCCAAGCCGATAATCCCGGCCTTGAAAAGGGTGCGGCGGGTTTGTTGAGATAACACAATTA
TCAAGAGGAAGTTCGACAAT

Downstream 100 bases:

>100_bases
GAGTCTTCCGGACTATGACACTTCAACAATTTTTACTCATCCTTCGTGCCCGGTATAAGGTAGTCCTCTATATTTTATTG
TTTACGGTAATCGCTACCCT

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MFQILVRTLILLSMLFSVKVFAESPDYPLGPGDILRIQVFQNPDLTTETRVSESGSITFPLVGALEVGGLSVASAENKLA
AALKKGGFIKQPQVTIVLLQMRGSQVSVLGQVNRPGRFPLETLSRVSDMLAAAGGTTPLGDDFAIVTGTRNGQAFRKVID
IPALYLEERSDEDIILAGGDTIYVHRAPVFYIYGEAQRPGAYRIERGMTVMQALAQGGGPTPRGSEWWLRLHRRNADGVV
EKLSPDMTDAVQPNDIIYVRESIF

Sequences:

>Translated_264_residues
MFQILVRTLILLSMLFSVKVFAESPDYPLGPGDILRIQVFQNPDLTTETRVSESGSITFPLVGALEVGGLSVASAENKLA
AALKKGGFIKQPQVTIVLLQMRGSQVSVLGQVNRPGRFPLETLSRVSDMLAAAGGTTPLGDDFAIVTGTRNGQAFRKVID
IPALYLEERSDEDIILAGGDTIYVHRAPVFYIYGEAQRPGAYRIERGMTVMQALAQGGGPTPRGSEWWLRLHRRNADGVV
EKLSPDMTDAVQPNDIIYVRESIF
>Mature_264_residues
MFQILVRTLILLSMLFSVKVFAESPDYPLGPGDILRIQVFQNPDLTTETRVSESGSITFPLVGALEVGGLSVASAENKLA
AALKKGGFIKQPQVTIVLLQMRGSQVSVLGQVNRPGRFPLETLSRVSDMLAAAGGTTPLGDDFAIVTGTRNGQAFRKVID
IPALYLEERSDEDIILAGGDTIYVHRAPVFYIYGEAQRPGAYRIERGMTVMQALAQGGGPTPRGSEWWLRLHRRNADGVV
EKLSPDMTDAVQPNDIIYVRESIF

Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787218, Length=209, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 28791; Mature: 28791

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQILVRTLILLSMLFSVKVFAESPDYPLGPGDILRIQVFQNPDLTTETRVSESGSITFP
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
LVGALEVGGLSVASAENKLAAALKKGGFIKQPQVTIVLLQMRGSQVSVLGQVNRPGRFPL
EEEEEEECCEEEECCHHHHHHHHHCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCH
ETLSRVSDMLAAAGGTTPLGDDFAIVTGTRNGQAFRKVIDIPALYLEERSDEDIILAGGD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCCCCEEEECCC
TIYVHRAPVFYIYGEAQRPGAYRIERGMTVMQALAQGGGPTPRGSEWWLRLHRRNADGVV
EEEEEECEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCHH
EKLSPDMTDAVQPNDIIYVRESIF
HHCCCCCHHCCCCCCEEEEEECCC
>Mature Secondary Structure
MFQILVRTLILLSMLFSVKVFAESPDYPLGPGDILRIQVFQNPDLTTETRVSESGSITFP
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
LVGALEVGGLSVASAENKLAAALKKGGFIKQPQVTIVLLQMRGSQVSVLGQVNRPGRFPL
EEEEEEECCEEEECCHHHHHHHHHCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCH
ETLSRVSDMLAAAGGTTPLGDDFAIVTGTRNGQAFRKVIDIPALYLEERSDEDIILAGGD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCCCCEEEECCC
TIYVHRAPVFYIYGEAQRPGAYRIERGMTVMQALAQGGGPTPRGSEWWLRLHRRNADGVV
EEEEEECEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHEEEEECCCCCCHH
EKLSPDMTDAVQPNDIIYVRESIF
HHCCCCCHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]