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The map label for this gene is mycA [H]

Identifier: 82701365

GI number: 82701365

Start: 244769

End: 246595

Strand: Reverse

Name: mycA [H]

Synonym: Nmul_A0231

Alternate gene names: 82701365

Gene position: 246595-244769 (Counterclockwise)

Preceding gene: 82701366

Following gene: 82701364

Centisome position: 7.74

GC content: 56.05

Gene sequence:

>1827_bases
TTGAGTTCAACAATAGCAAAGTTGAACGCTCCGCTGACGCAAGCGTTTAACCCTCAATTATCAAGCGAGCAGCCGGCAGC
TGCGATCCCCACACCCACCTACAACACTCAAGCTCAGAGATTAGCCGACTTCGACACCTTGATCGCTGCGCTCGAGTATG
CGGCGGCAGGTACAGCCGGATATAACTTTTACGATGCCAAGGGTAATCTCCGCTCTGTTCTTCCCTATCAGGCATTAAGG
CAAAATGCCCAAACAGCGGCGCGGCAGTTGCTGCGGCTTGGGTTGGCGAGAGGAGACCGTGTTGCGATTATTGCCGACAC
GACGCCGGAGTTCGTGGAGATCTTTTATGCATGCCGCTATGCAGGGCTGGTACCTTATGCAATGCCTGTTCCTGTCAACC
TCGGCAGCCATGCGGTTTATGTTCGGCAGTTGCGCGGAATGCTGGAAGCGGGCCAGGCAAGCGCCGCTCTGGCGAACGCC
GACTATGTCGATTTCTTGAAAGAAGCGGCCGAGGGGTCCCAATATCTGCGTTGGGTGGGAACGCCGGAGCAACTCGGGGA
ACTGCCGGCGTCGATCACCGAGCTTGCGCCAAATAGTCCGGATGAAATCGCATATCTGCAATTTACTTCGGGAAGCACGC
GCATGCCCCGTGGAGTAGTAATTACCGAAGGTGCACTAATGAGCAACCTTCAGGGCATTGTCCGTCACGGTCTGAATATC
CGGGCAGGAGACCGCTGCGCATCCTGGTTGCCGTTTTATCATGACATGGGCTTGGTTGGCATGGTAATGGCCCCCATGGT
GGCGCAAGTTTCCGTTGATTATCTGGCTACCCGTGATTTTGCAATCAGGCCGCTGCAATGGCTGCGGCTGATCAGCAGAA
ATCGCGCTACCGTCGCATTCAGCCAACCTTTTGGACTCAAGCTCTGCACGCTTCGCGTGCGCGATTCCGACTTGGAAGAT
CTTGACCTCAGCAGTTGGCGTGCAGCGGGAGTCGGCGCGGAAATGATCCGTCCGGACACTTTGAGGAATTTTGCGGAAAA
ATTTGCGCCTGCCGGTTTTGACGCGCGCGCATTCCTCCCCTGCTACGGGCTGGCTGAATCGACGCTCGCCGTTACCTTCT
CCGAAGTTGGCCAAGGTTTTCAGAGTTTGCGCGTCGACGCGGCAACCCTCGTTGACAGGAAAATGGCGGTACGGTTACAG
GTGGAAGGCCGGAAGTATAGTGAATTTGTCAATTGCGGCCGCGCCTTGCCGGGACATATCGTCAAAGTGATCGACGAGGC
CGGCCAGCAACTCCCGGATTTGCGAGTAGGCAGCGTACTCGTGCAGGGCAGCAGTATCATGACAGGTTATTTCAACAATC
CGGAAGATACCGGCAAAACACTCAAACCCGGAAACTGGCTGGATACCGGTGATCTGGGGTTCCTCTTCGAAGGGGATCTT
TACATCACCGGGCGCCGCACCGATGTAATCATCGTCAATGGGCGTAACATCCGCGCGCAGGATATCGAGGAGCTTGCCGA
ACAGCAACCTGAAGTGAGAACGCGCGAAGCGTCCGCCTTCGGCATTACTGACGCCAATGACGTCACGACCATTGTCCTGG
TCATCGAATGCCGACTTACCTCGATAACGGATCGCCAGTCCCTTACCAGCCGTCTGCAACGTCTGGTCTACATGGCGTTT
GGCGTGAATTGTCTGGTGGAACTGGTTCCACCGCACACCCTGCCGCGTACTTCCTCTGGAAAACTGTCCCGTTTTGCGGC
CCGCCAGGGCTTTATCCAGCGAACGCAGATGACAGACCAGTTGTCTGCCGAATCCGGGGACAAATAA

Upstream 100 bases:

>100_bases
ACGACGCGACAGCGGATGATGGCCAAAAACACGATTGCCCGACTGGAATCCTTTATATTATAAAAAGATGACTAGTACGA
TTAACTTGGAGGGGAAGACC

Downstream 100 bases:

>100_bases
TCGGGGTATGTCGGAGTCCTGGGTTGCCGTTACCGGCGCCACTGGCTTTATCGGAAATGCTCTGCTGCAGTCCCTGATAA
AGGAAGGATGGAAAGTCAGG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSSTIAKLNAPLTQAFNPQLSSEQPAAAIPTPTYNTQAQRLADFDTLIAALEYAAAGTAGYNFYDAKGNLRSVLPYQALR
QNAQTAARQLLRLGLARGDRVAIIADTTPEFVEIFYACRYAGLVPYAMPVPVNLGSHAVYVRQLRGMLEAGQASAALANA
DYVDFLKEAAEGSQYLRWVGTPEQLGELPASITELAPNSPDEIAYLQFTSGSTRMPRGVVITEGALMSNLQGIVRHGLNI
RAGDRCASWLPFYHDMGLVGMVMAPMVAQVSVDYLATRDFAIRPLQWLRLISRNRATVAFSQPFGLKLCTLRVRDSDLED
LDLSSWRAAGVGAEMIRPDTLRNFAEKFAPAGFDARAFLPCYGLAESTLAVTFSEVGQGFQSLRVDAATLVDRKMAVRLQ
VEGRKYSEFVNCGRALPGHIVKVIDEAGQQLPDLRVGSVLVQGSSIMTGYFNNPEDTGKTLKPGNWLDTGDLGFLFEGDL
YITGRRTDVIIVNGRNIRAQDIEELAEQQPEVRTREASAFGITDANDVTTIVLVIECRLTSITDRQSLTSRLQRLVYMAF
GVNCLVELVPPHTLPRTSSGKLSRFAARQGFIQRTQMTDQLSAESGDK

Sequences:

>Translated_608_residues
MSSTIAKLNAPLTQAFNPQLSSEQPAAAIPTPTYNTQAQRLADFDTLIAALEYAAAGTAGYNFYDAKGNLRSVLPYQALR
QNAQTAARQLLRLGLARGDRVAIIADTTPEFVEIFYACRYAGLVPYAMPVPVNLGSHAVYVRQLRGMLEAGQASAALANA
DYVDFLKEAAEGSQYLRWVGTPEQLGELPASITELAPNSPDEIAYLQFTSGSTRMPRGVVITEGALMSNLQGIVRHGLNI
RAGDRCASWLPFYHDMGLVGMVMAPMVAQVSVDYLATRDFAIRPLQWLRLISRNRATVAFSQPFGLKLCTLRVRDSDLED
LDLSSWRAAGVGAEMIRPDTLRNFAEKFAPAGFDARAFLPCYGLAESTLAVTFSEVGQGFQSLRVDAATLVDRKMAVRLQ
VEGRKYSEFVNCGRALPGHIVKVIDEAGQQLPDLRVGSVLVQGSSIMTGYFNNPEDTGKTLKPGNWLDTGDLGFLFEGDL
YITGRRTDVIIVNGRNIRAQDIEELAEQQPEVRTREASAFGITDANDVTTIVLVIECRLTSITDRQSLTSRLQRLVYMAF
GVNCLVELVPPHTLPRTSSGKLSRFAARQGFIQRTQMTDQLSAESGDK
>Mature_607_residues
SSTIAKLNAPLTQAFNPQLSSEQPAAAIPTPTYNTQAQRLADFDTLIAALEYAAAGTAGYNFYDAKGNLRSVLPYQALRQ
NAQTAARQLLRLGLARGDRVAIIADTTPEFVEIFYACRYAGLVPYAMPVPVNLGSHAVYVRQLRGMLEAGQASAALANAD
YVDFLKEAAEGSQYLRWVGTPEQLGELPASITELAPNSPDEIAYLQFTSGSTRMPRGVVITEGALMSNLQGIVRHGLNIR
AGDRCASWLPFYHDMGLVGMVMAPMVAQVSVDYLATRDFAIRPLQWLRLISRNRATVAFSQPFGLKLCTLRVRDSDLEDL
DLSSWRAAGVGAEMIRPDTLRNFAEKFAPAGFDARAFLPCYGLAESTLAVTFSEVGQGFQSLRVDAATLVDRKMAVRLQV
EGRKYSEFVNCGRALPGHIVKVIDEAGQQLPDLRVGSVLVQGSSIMTGYFNNPEDTGKTLKPGNWLDTGDLGFLFEGDLY
ITGRRTDVIIVNGRNIRAQDIEELAEQQPEVRTREASAFGITDANDVTTIVLVIECRLTSITDRQSLTSRLQRLVYMAFG
VNCLVELVPPHTLPRTSSGKLSRFAARQGFIQRTQMTDQLSAESGDK

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=491, Percent_Identity=25.4582484725051, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI45827692, Length=491, Percent_Identity=26.0692464358452, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI225735629, Length=491, Percent_Identity=26.0692464358452, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI156151445, Length=563, Percent_Identity=20.9591474245115, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI55749758, Length=583, Percent_Identity=23.4991423670669, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI225735625, Length=556, Percent_Identity=24.1007194244604, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI145693145, Length=548, Percent_Identity=25.3649635036496, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788107, Length=562, Percent_Identity=21.7081850533808, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=560, Percent_Identity=22.5, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193202679, Length=548, Percent_Identity=22.992700729927, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=489, Percent_Identity=19.8364008179959, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581924, Length=401, Percent_Identity=24.9376558603491, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654656, Length=601, Percent_Identity=22.6289517470882, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648260, Length=181, Percent_Identity=32.0441988950276, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20130357, Length=191, Percent_Identity=31.413612565445, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356441, Length=543, Percent_Identity=22.2836095764273, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356947, Length=523, Percent_Identity=22.9445506692161, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281365686, Length=470, Percent_Identity=22.3404255319149, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653035, Length=188, Percent_Identity=26.5957446808511, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24656500, Length=198, Percent_Identity=30.3030303030303, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: NA

Molecular weight: Translated: 66527; Mature: 66395

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTIAKLNAPLTQAFNPQLSSEQPAAAIPTPTYNTQAQRLADFDTLIAALEYAAAGTAG
CCCCHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
YNFYDAKGNLRSVLPYQALRQNAQTAARQLLRLGLARGDRVAIIADTTPEFVEIFYACRY
CEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
AGLVPYAMPVPVNLGSHAVYVRQLRGMLEAGQASAALANADYVDFLKEAAEGSQYLRWVG
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCEEEECC
TPEQLGELPASITELAPNSPDEIAYLQFTSGSTRMPRGVVITEGALMSNLQGIVRHGLNI
CHHHHHHCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHCCCCC
RAGDRCASWLPFYHDMGLVGMVMAPMVAQVSVDYLATRDFAIRPLQWLRLISRNRATVAF
CCCCHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEE
SQPFGLKLCTLRVRDSDLEDLDLSSWRAAGVGAEMIRPDTLRNFAEKFAPAGFDARAFLP
CCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCHHHCCCHHHHHHHHHHCCCCCCCCEECC
CYGLAESTLAVTFSEVGQGFQSLRVDAATLVDRKMAVRLQVEGRKYSEFVNCGRALPGHI
CCCCCCHHHEEEHHHHHCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCCCHHHH
VKVIDEAGQQLPDLRVGSVLVQGSSIMTGYFNNPEDTGKTLKPGNWLDTGDLGFLFEGDL
HHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCE
YITGRRTDVIIVNGRNIRAQDIEELAEQQPEVRTREASAFGITDANDVTTIVLVIECRLT
EEECCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCCEECCCCCCCCEEEEEEEEECCC
SITDRQSLTSRLQRLVYMAFGVNCLVELVPPHTLPRTSSGKLSRFAARQGFIQRTQMTDQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LSAESGDK
HCCCCCCC
>Mature Secondary Structure 
SSTIAKLNAPLTQAFNPQLSSEQPAAAIPTPTYNTQAQRLADFDTLIAALEYAAAGTAG
CCCHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
YNFYDAKGNLRSVLPYQALRQNAQTAARQLLRLGLARGDRVAIIADTTPEFVEIFYACRY
CEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
AGLVPYAMPVPVNLGSHAVYVRQLRGMLEAGQASAALANADYVDFLKEAAEGSQYLRWVG
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCCEEEECC
TPEQLGELPASITELAPNSPDEIAYLQFTSGSTRMPRGVVITEGALMSNLQGIVRHGLNI
CHHHHHHCCHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHCCCCC
RAGDRCASWLPFYHDMGLVGMVMAPMVAQVSVDYLATRDFAIRPLQWLRLISRNRATVAF
CCCCHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEE
SQPFGLKLCTLRVRDSDLEDLDLSSWRAAGVGAEMIRPDTLRNFAEKFAPAGFDARAFLP
CCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCHHHCCCHHHHHHHHHHCCCCCCCCEECC
CYGLAESTLAVTFSEVGQGFQSLRVDAATLVDRKMAVRLQVEGRKYSEFVNCGRALPGHI
CCCCCCHHHEEEHHHHHCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCCCHHHH
VKVIDEAGQQLPDLRVGSVLVQGSSIMTGYFNNPEDTGKTLKPGNWLDTGDLGFLFEGDL
HHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCE
YITGRRTDVIIVNGRNIRAQDIEELAEQQPEVRTREASAFGITDANDVTTIVLVIECRLT
EEECCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCCCEECCCCCCCCEEEEEEEEECCC
SITDRQSLTSRLQRLVYMAFGVNCLVELVPPHTLPRTSSGKLSRFAARQGFIQRTQMTDQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LSAESGDK
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]