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The map label for this gene is pyrE

Identifier: 82701359

GI number: 82701359

Start: 239636

End: 240322

Strand: Direct

Name: pyrE

Synonym: Nmul_A0225

Alternate gene names: 82701359

Gene position: 239636-240322 (Clockwise)

Preceding gene: 82701356

Following gene: 82701361

Centisome position: 7.53

GC content: 56.91

Gene sequence:

>687_bases
ATGTCCGATTTTCGAAAGGAGTTCATCGAGTTCGCCATCGGCCGGAATGTTCTGTGCTTTGGCGAATTCCAAACCAAAGC
AGGACGTATTTCGCCGTATTTTTTTAATGCGGGACTGTTCAACGATGGGGAATCTCTCGGAAAGCTGGGGCAATTTTACG
CCAAAGCCATCCTTGCTGCCCAGCTTCCGTTTGACATGCTTTTCGGTCCCGCCTATAAGGGCATTCCACTCGTAAGTTCC
ATTGCCATCGCCCTGGCCGACGGGGGCAACAACTACCCCTTCTGTTTCAACCGCAAAGAGGCGAAAGACCACGGAGAAGG
AGGAAATCTCGTGGGCGCTCCTCTCGCCGGGCGGGTGCTGATCGTGGATGATGTGATTTCCGCCGGCACATCGGTGCGGG
AATCGGTCGAGTATATTCATGCCGCAGGTGCCACCCCCGCAGGGGTGGCCATCGCCCTGGACCGCATGGAGCGGGGCAAA
GGCGAGCTTTCCGCCGTGCAGGAAGTGCGCTGCATGTATGGAATTCCCGTCACGAGCATCGTCAATCTGGAAAACATTGT
CGATTACCTGCGTGAGCAGGGGAATCTGGCGCATCACCTGCCGGCTGTGGAGGAATATCGCGCCCGGTATTCCTCCAGAA
TGGTAGAACCGATTGAATGCGGAGGCGGCACTCCACGCGCGGGCTGA

Upstream 100 bases:

>100_bases
TGCAAAGGCAGCCATCGGAAAAAACCCTTGTTGGCAGCAGAGCGTATTCCGTTGAGGTTAAGCGTTATAATTCGCACGTC
GATTTCCCGAAGCCATTATC

Downstream 100 bases:

>100_bases
GGGTTTTTTACCGGGCCCCCTCCGCCGGCAGGGCGGGGAGTGTGATTTCACCCTTTACATCAAAATGGCGGCCGATGGCA
AAGCCCGGCGCACCCAGCTG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSDFRKEFIEFAIGRNVLCFGEFQTKAGRISPYFFNAGLFNDGESLGKLGQFYAKAILAAQLPFDMLFGPAYKGIPLVSS
IAIALADGGNNYPFCFNRKEAKDHGEGGNLVGAPLAGRVLIVDDVISAGTSVRESVEYIHAAGATPAGVAIALDRMERGK
GELSAVQEVRCMYGIPVTSIVNLENIVDYLREQGNLAHHLPAVEEYRARYSSRMVEPIECGGGTPRAG

Sequences:

>Translated_228_residues
MSDFRKEFIEFAIGRNVLCFGEFQTKAGRISPYFFNAGLFNDGESLGKLGQFYAKAILAAQLPFDMLFGPAYKGIPLVSS
IAIALADGGNNYPFCFNRKEAKDHGEGGNLVGAPLAGRVLIVDDVISAGTSVRESVEYIHAAGATPAGVAIALDRMERGK
GELSAVQEVRCMYGIPVTSIVNLENIVDYLREQGNLAHHLPAVEEYRARYSSRMVEPIECGGGTPRAG
>Mature_227_residues
SDFRKEFIEFAIGRNVLCFGEFQTKAGRISPYFFNAGLFNDGESLGKLGQFYAKAILAAQLPFDMLFGPAYKGIPLVSSI
AIALADGGNNYPFCFNRKEAKDHGEGGNLVGAPLAGRVLIVDDVISAGTSVRESVEYIHAAGATPAGVAIALDRMERGKG
ELSAVQEVRCMYGIPVTSIVNLENIVDYLREQGNLAHHLPAVEEYRARYSSRMVEPIECGGGTPRAG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=179, Percent_Identity=28.4916201117318, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1790073, Length=211, Percent_Identity=57.8199052132701, Blast_Score=258, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17508631, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=48.1818181818182, Blast_Score=187, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=43.4389140271493, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_NITMU (Q2YCI8)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_410925.1
- ProteinModelPortal:   Q2YCI8
- SMR:   Q2YCI8
- STRING:   Q2YCI8
- GeneID:   3784602
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0225
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   Q2YCI8
- ProtClustDB:   PRK00455
- BioCyc:   NMUL323848:NMUL_A0225-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 24569; Mature: 24438

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 98-98 BINDING 99-99 BINDING 102-102 BINDING 104-104 BINDING 127-127 BINDING 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFRKEFIEFAIGRNVLCFGEFQTKAGRISPYFFNAGLFNDGESLGKLGQFYAKAILAA
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
QLPFDMLFGPAYKGIPLVSSIAIALADGGNNYPFCFNRKEAKDHGEGGNLVGAPLAGRVL
HCCHHHHHCCCCCCCHHHHHHHEEEECCCCCCCEEECCCHHHCCCCCCCEECCCCCCCEE
IVDDVISAGTSVRESVEYIHAAGATPAGVAIALDRMERGKGELSAVQEVRCMYGIPVTSI
EEEHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHH
VNLENIVDYLREQGNLAHHLPAVEEYRARYSSRMVEPIECGGGTPRAG
HHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
SDFRKEFIEFAIGRNVLCFGEFQTKAGRISPYFFNAGLFNDGESLGKLGQFYAKAILAA
CHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
QLPFDMLFGPAYKGIPLVSSIAIALADGGNNYPFCFNRKEAKDHGEGGNLVGAPLAGRVL
HCCHHHHHCCCCCCCHHHHHHHEEEECCCCCCCEEECCCHHHCCCCCCCEECCCCCCCEE
IVDDVISAGTSVRESVEYIHAAGATPAGVAIALDRMERGKGELSAVQEVRCMYGIPVTSI
EEEHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHH
VNLENIVDYLREQGNLAHHLPAVEEYRARYSSRMVEPIECGGGTPRAG
HHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA