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The map label for this gene is 82701350

Identifier: 82701350

GI number: 82701350

Start: 230469

End: 231764

Strand: Direct

Name: 82701350

Synonym: Nmul_A0216

Alternate gene names: NA

Gene position: 230469-231764 (Clockwise)

Preceding gene: 82701349

Following gene: 82701351

Centisome position: 7.24

GC content: 54.17

Gene sequence:

>1296_bases
ATGCCTACGCCCCACCTTGATACTGCCTTGAGCGGCCCCATTCTTGATCTTGAAAGATGCATCCTCAATGCCATGCCTTC
GATCGAGCAATGGTTGCGCAATGAATGGCAGACGCATGCTGCGCCTTTTTACTGCTCCGTGGATTTGCGCAATAGCGGGT
TCAAGCTGGCGCCCGTCGATACCAACCTGTTTCCAGGCGGCTTCAATAATCTAAACCGCGAATTCATGCCCTTGTGCGTC
CAGGCGATGATGGCGGCAATCGATAAAATCTGTACCGGCGCGCGCAGCGTACTGCTGATTCCGGAAAGCCACACGCGCAA
TATTTTTTATTTGCAAAATCTGGCGGCCTTGCACGCAATCATGCGGCATGCCGGCATTCATGTCCGTATCGGAACGCTGC
TCCCTGAGATCACCGCACCCACTGCGCTCGACCTGCCCGGTGGAAACAAGCTCACGCTCGAACCCATTCAGCGGAAGGGC
AATCGGGTCGTTCTGGAAGATTTCGATCCTTGCGTCGTGCTTCTCAATAACGACCTTTCCACCGGCACCCCGGCCGTCCT
GCAGAATCTCGAGCAGACGGTCATTCCTCCCCTGCATGCGGGATGGACCAGCCGGCGGAAATCCCATCATTTCACTGCCT
ATGATAACGTTTCGCAGCAGTTTGCGAGCCTGATCGGTATTGACCCGTGGCTTATCAATCCTTATTTCGCTTCCTGCGGA
AAAATCAATTTTCGCGAGAAAAAGGGTGAAGACTGCGTGGCCAATACGGTGGATGAAATCCTGCACCAGATCCGGGAGAA
ATATGCCGAGTATGGAGTCAGGAAAGATCCCTTCGTGATCGTAAAGGCCGACGCCGGTACGTACGGAATGGGGATAATGA
CTGTAAAAGATGGCGCGCAAGTACGTACACTCAGCCGGAAACAGCGCAACAAGATGGCGGTCGTAAAAGAAGGATTGGAG
GTGACCGACATCATGGTGCAGGAAGGGGTTTATACGTTTGAGAATGTCGACGATGCGGTGGCGGAGCCCGTCATCTATAT
GATCGATCGCTATGTCGTCGGTGGTTTCTACCGGGTGCATACCGAGCGGGGCGTTGACGAGAATCTCAACGCCCCCGGTA
TGCATTTTGTACCCCTGGCATTCGAAGATACCTGTCTGCTACCGGATCGGGAAGCGCAGCCGGGTTGCAGCGCCAACCGG
TTTTATGCTTATGGCGTCATAGCCAGGCTCGCTTTACTGGCGGCTGCACAAGAACTGGAAAAAAGTGAGGCGAGAATCGA
AGCGATCATGGCTTAG

Upstream 100 bases:

>100_bases
TGCCCGCGTGCCCCAGCTTTATGGCTTTATGGAAGGGGCGGCTTATTCCCTGCTTTCCTGATGTTACAATCGCCGTTTCC
TGTTGATACCCGCTTCCTTC

Downstream 100 bases:

>100_bases
CCCAGTCTCATTCATTCATTTTCTCGGGATTTTCCTTTGGGAAGTTCCTCTGAAAAAGAATCCCAAAAATGGACAGCGGC
TTATCTGCAGGGGGAGTTCG

Product: glutamate--cysteine ligase GshA

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPTPHLDTALSGPILDLERCILNAMPSIEQWLRNEWQTHAAPFYCSVDLRNSGFKLAPVDTNLFPGGFNNLNREFMPLCV
QAMMAAIDKICTGARSVLLIPESHTRNIFYLQNLAALHAIMRHAGIHVRIGTLLPEITAPTALDLPGGNKLTLEPIQRKG
NRVVLEDFDPCVVLLNNDLSTGTPAVLQNLEQTVIPPLHAGWTSRRKSHHFTAYDNVSQQFASLIGIDPWLINPYFASCG
KINFREKKGEDCVANTVDEILHQIREKYAEYGVRKDPFVIVKADAGTYGMGIMTVKDGAQVRTLSRKQRNKMAVVKEGLE
VTDIMVQEGVYTFENVDDAVAEPVIYMIDRYVVGGFYRVHTERGVDENLNAPGMHFVPLAFEDTCLLPDREAQPGCSANR
FYAYGVIARLALLAAAQELEKSEARIEAIMA

Sequences:

>Translated_431_residues
MPTPHLDTALSGPILDLERCILNAMPSIEQWLRNEWQTHAAPFYCSVDLRNSGFKLAPVDTNLFPGGFNNLNREFMPLCV
QAMMAAIDKICTGARSVLLIPESHTRNIFYLQNLAALHAIMRHAGIHVRIGTLLPEITAPTALDLPGGNKLTLEPIQRKG
NRVVLEDFDPCVVLLNNDLSTGTPAVLQNLEQTVIPPLHAGWTSRRKSHHFTAYDNVSQQFASLIGIDPWLINPYFASCG
KINFREKKGEDCVANTVDEILHQIREKYAEYGVRKDPFVIVKADAGTYGMGIMTVKDGAQVRTLSRKQRNKMAVVKEGLE
VTDIMVQEGVYTFENVDDAVAEPVIYMIDRYVVGGFYRVHTERGVDENLNAPGMHFVPLAFEDTCLLPDREAQPGCSANR
FYAYGVIARLALLAAAQELEKSEARIEAIMA
>Mature_430_residues
PTPHLDTALSGPILDLERCILNAMPSIEQWLRNEWQTHAAPFYCSVDLRNSGFKLAPVDTNLFPGGFNNLNREFMPLCVQ
AMMAAIDKICTGARSVLLIPESHTRNIFYLQNLAALHAIMRHAGIHVRIGTLLPEITAPTALDLPGGNKLTLEPIQRKGN
RVVLEDFDPCVVLLNNDLSTGTPAVLQNLEQTVIPPLHAGWTSRRKSHHFTAYDNVSQQFASLIGIDPWLINPYFASCGK
INFREKKGEDCVANTVDEILHQIREKYAEYGVRKDPFVIVKADAGTYGMGIMTVKDGAQVRTLSRKQRNKMAVVKEGLEV
TDIMVQEGVYTFENVDDAVAEPVIYMIDRYVVGGFYRVHTERGVDENLNAPGMHFVPLAFEDTCLLPDREAQPGCSANRF
YAYGVIARLALLAAAQELEKSEARIEAIMA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47952; Mature: 47821

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPHLDTALSGPILDLERCILNAMPSIEQWLRNEWQTHAAPFYCSVDLRNSGFKLAPVD
CCCCCCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECC
TNLFPGGFNNLNREFMPLCVQAMMAAIDKICTGARSVLLIPESHTRNIFYLQNLAALHAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHH
MRHAGIHVRIGTLLPEITAPTALDLPGGNKLTLEPIQRKGNRVVLEDFDPCVVLLNNDLS
HHHCCCEEEEHHHCCCCCCCCEEECCCCCEEEEHHHHHCCCEEEEECCCCEEEEEECCCC
TGTPAVLQNLEQTVIPPLHAGWTSRRKSHHFTAYDNVSQQFASLIGIDPWLINPYFASCG
CCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCC
KINFREKKGEDCVANTVDEILHQIREKYAEYGVRKDPFVIVKADAGTYGMGIMTVKDGAQ
CCEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCH
VRTLSRKQRNKMAVVKEGLEVTDIMVQEGVYTFENVDDAVAEPVIYMIDRYVVGGFYRVH
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCEEEEE
TERGVDENLNAPGMHFVPLAFEDTCLLPDREAQPGCSANRFYAYGVIARLALLAAAQELE
CCCCCCCCCCCCCCEEEEEEECCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KSEARIEAIMA
HHHHHHHHHCC
>Mature Secondary Structure 
PTPHLDTALSGPILDLERCILNAMPSIEQWLRNEWQTHAAPFYCSVDLRNSGFKLAPVD
CCCCCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECC
TNLFPGGFNNLNREFMPLCVQAMMAAIDKICTGARSVLLIPESHTRNIFYLQNLAALHAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHH
MRHAGIHVRIGTLLPEITAPTALDLPGGNKLTLEPIQRKGNRVVLEDFDPCVVLLNNDLS
HHHCCCEEEEHHHCCCCCCCCEEECCCCCEEEEHHHHHCCCEEEEECCCCEEEEEECCCC
TGTPAVLQNLEQTVIPPLHAGWTSRRKSHHFTAYDNVSQQFASLIGIDPWLINPYFASCG
CCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCC
KINFREKKGEDCVANTVDEILHQIREKYAEYGVRKDPFVIVKADAGTYGMGIMTVKDGAQ
CCEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCH
VRTLSRKQRNKMAVVKEGLEVTDIMVQEGVYTFENVDDAVAEPVIYMIDRYVVGGFYRVH
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCEEEEE
TERGVDENLNAPGMHFVPLAFEDTCLLPDREAQPGCSANRFYAYGVIARLALLAAAQELE
CCCCCCCCCCCCCCEEEEEEECCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KSEARIEAIMA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA