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The map label for this gene is sbcD [H]

Identifier: 82701337

GI number: 82701337

Start: 214241

End: 215470

Strand: Direct

Name: sbcD [H]

Synonym: Nmul_A0203

Alternate gene names: 82701337

Gene position: 214241-215470 (Clockwise)

Preceding gene: 82701336

Following gene: 82701338

Centisome position: 6.73

GC content: 54.39

Gene sequence:

>1230_bases
ATGAAACTCTTCCATACCTCCGACTGGCACATCGGCCGGACCCTATATGGCAGAAAGCGGTACGAGGAATTCGAGGCATT
TCTGACGTGGCTGGCAGAAACGATTCAGAGGAACGAAATCGATGTCCTCCTGGTAGCCGGTGATATCTTTGATACCAGTG
CTCCAAGCAATCGTGCCCAGGAGCTCTACTACCGCTTTCTCTGGAAGATAGCAGCTTCACCCTGCCGCCATGTGGTCATC
GTCGCGGGTAATCACGATTCCCCTTCATTTCTGAATGCACCCAGGGCGCTGCTCAAAGCCCTTGATGTCCATGTGATCGG
TAGCCCCTCAGCATCCCTTGAAGATGAAGTACTGGTACTCCGAAATGAGCAAGGGGTGCCCGAGCTAATTGTTTGCGCCG
TGCCGTATCTTCGTGACAAAGACATCCGCGTGGCGGAAGCCGGCGAGAGTGTCGAGGATAAGGAACGGAAGCTGCTCGTC
GGTATTCGCGATCACTACGCTGCCATAGCCGCCCTGGCTGAACAGAAGCGTGTGGAACTCGGGGTGGATATTCCCATCGT
TGCCACGGGCCACCTTTTCGCCGCCGGAGGGCAAACTATCGAGGGTGACGGCGTGCGGGACCTGTATGTCGGTTCGCTGG
CTCAGGTGAGTGCGGGGATTTTTCCTGAATGCTTCAACTACCTTGCGCTGGGCCACCTCCATGTCCCGCAGAAGGTAAAC
GGTTCCGAGATCATGCGATACAGTGGCTCTCCTCTCCCCATGGGGTTCGGGGAGGCCAGACAACAGAAGAGCGTTTGCCA
GGTTGAATTCCACAGTACCGCCGCGTCTGTTCAATTGATCGATGTGCCGGTGTTTCAGAAGCTCGAACGTATCAAGGGAG
ACTGGGACAGCATCTCCACTCAGATTCGTAAACTGTCAGAGACCGGCTCTCAAGCATGGCTCGAAGTCATCTACGAAGGT
GAAGAAGTAATCGGCGACCTGCGCGAGCGCCTGGAAATCGCCATTACCGACACCCAAATGGAAATTCTGCGGGTGAAGAA
CAACCCTGTTATCGATCGCGTCCTTGGAAAAATCCATGAAGAAGAAACACTGGACGATCTCAGTGTGAATGACGTATTCG
AGCGATGCCTGACCGTACATGAAGTGCCTGAAGACCAGCGGCCAGAATTACTTCGTGCTTATCAGGAAGCGCTTTCGTCT
CTCTATGAGGACGACATGCAAGCGGAATAG

Upstream 100 bases:

>100_bases
ACCTGAGTCAGGAGAGATCTTCTGGCCGATATGATGAGACTCAAAAATTAGCGGGAGGAATACATGGAATGCTTCGTCCC
TTCGGCCGCTGGATAACAGG

Downstream 100 bases:

>100_bases
AGAGGCTGTCGATGAAGATATTGCAGGTACGATTCAAGAATCTGAACTCGCTGATGGGTGAATGGGAAATCGACCTGACG
CATCCCGCCTTTACCTCCGA

Product: nuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKLFHTSDWHIGRTLYGRKRYEEFEAFLTWLAETIQRNEIDVLLVAGDIFDTSAPSNRAQELYYRFLWKIAASPCRHVVI
VAGNHDSPSFLNAPRALLKALDVHVIGSPSASLEDEVLVLRNEQGVPELIVCAVPYLRDKDIRVAEAGESVEDKERKLLV
GIRDHYAAIAALAEQKRVELGVDIPIVATGHLFAAGGQTIEGDGVRDLYVGSLAQVSAGIFPECFNYLALGHLHVPQKVN
GSEIMRYSGSPLPMGFGEARQQKSVCQVEFHSTAASVQLIDVPVFQKLERIKGDWDSISTQIRKLSETGSQAWLEVIYEG
EEVIGDLRERLEIAITDTQMEILRVKNNPVIDRVLGKIHEEETLDDLSVNDVFERCLTVHEVPEDQRPELLRAYQEALSS
LYEDDMQAE

Sequences:

>Translated_409_residues
MKLFHTSDWHIGRTLYGRKRYEEFEAFLTWLAETIQRNEIDVLLVAGDIFDTSAPSNRAQELYYRFLWKIAASPCRHVVI
VAGNHDSPSFLNAPRALLKALDVHVIGSPSASLEDEVLVLRNEQGVPELIVCAVPYLRDKDIRVAEAGESVEDKERKLLV
GIRDHYAAIAALAEQKRVELGVDIPIVATGHLFAAGGQTIEGDGVRDLYVGSLAQVSAGIFPECFNYLALGHLHVPQKVN
GSEIMRYSGSPLPMGFGEARQQKSVCQVEFHSTAASVQLIDVPVFQKLERIKGDWDSISTQIRKLSETGSQAWLEVIYEG
EEVIGDLRERLEIAITDTQMEILRVKNNPVIDRVLGKIHEEETLDDLSVNDVFERCLTVHEVPEDQRPELLRAYQEALSS
LYEDDMQAE
>Mature_409_residues
MKLFHTSDWHIGRTLYGRKRYEEFEAFLTWLAETIQRNEIDVLLVAGDIFDTSAPSNRAQELYYRFLWKIAASPCRHVVI
VAGNHDSPSFLNAPRALLKALDVHVIGSPSASLEDEVLVLRNEQGVPELIVCAVPYLRDKDIRVAEAGESVEDKERKLLV
GIRDHYAAIAALAEQKRVELGVDIPIVATGHLFAAGGQTIEGDGVRDLYVGSLAQVSAGIFPECFNYLALGHLHVPQKVN
GSEIMRYSGSPLPMGFGEARQQKSVCQVEFHSTAASVQLIDVPVFQKLERIKGDWDSISTQIRKLSETGSQAWLEVIYEG
EEVIGDLRERLEIAITDTQMEILRVKNNPVIDRVLGKIHEEETLDDLSVNDVFERCLTVHEVPEDQRPELLRAYQEALSS
LYEDDMQAE

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=405, Percent_Identity=35.8024691358025, Blast_Score=255, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45862; Mature: 45862

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFHTSDWHIGRTLYGRKRYEEFEAFLTWLAETIQRNEIDVLLVAGDIFDTSAPSNRAQ
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHH
ELYYRFLWKIAASPCRHVVIVAGNHDSPSFLNAPRALLKALDVHVIGSPSASLEDEVLVL
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEE
RNEQGVPELIVCAVPYLRDKDIRVAEAGESVEDKERKLLVGIRDHYAAIAALAEQKRVEL
ECCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GVDIPIVATGHLFAAGGQTIEGDGVRDLYVGSLAQVSAGIFPECFNYLALGHLHVPQKVN
CCCCCEEECCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEECCCCCC
GSEIMRYSGSPLPMGFGEARQQKSVCQVEFHSTAASVQLIDVPVFQKLERIKGDWDSIST
HHHHEEECCCCCCCCCCHHHHHCCCEEEEECCCCCEEEEEECHHHHHHHHHCCCHHHHHH
QIRKLSETGSQAWLEVIYEGEEVIGDLRERLEIAITDTQMEILRVKNNPVIDRVLGKIHE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEECCEEEEEEECCCCHHHHHHHHHHH
EETLDDLSVNDVFERCLTVHEVPEDQRPELLRAYQEALSSLYEDDMQAE
HHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLFHTSDWHIGRTLYGRKRYEEFEAFLTWLAETIQRNEIDVLLVAGDIFDTSAPSNRAQ
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHH
ELYYRFLWKIAASPCRHVVIVAGNHDSPSFLNAPRALLKALDVHVIGSPSASLEDEVLVL
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEE
RNEQGVPELIVCAVPYLRDKDIRVAEAGESVEDKERKLLVGIRDHYAAIAALAEQKRVEL
ECCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GVDIPIVATGHLFAAGGQTIEGDGVRDLYVGSLAQVSAGIFPECFNYLALGHLHVPQKVN
CCCCCEEECCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEECCCCCC
GSEIMRYSGSPLPMGFGEARQQKSVCQVEFHSTAASVQLIDVPVFQKLERIKGDWDSIST
HHHHEEECCCCCCCCCCHHHHHCCCEEEEECCCCCEEEEEECHHHHHHHHHCCCHHHHHH
QIRKLSETGSQAWLEVIYEGEEVIGDLRERLEIAITDTQMEILRVKNNPVIDRVLGKIHE
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEECCEEEEEEECCCCHHHHHHHHHHH
EETLDDLSVNDVFERCLTVHEVPEDQRPELLRAYQEALSSLYEDDMQAE
HHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]