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The map label for this gene is glcD [H]

Identifier: 82701327

GI number: 82701327

Start: 202579

End: 204015

Strand: Reverse

Name: glcD [H]

Synonym: Nmul_A0193

Alternate gene names: 82701327

Gene position: 204015-202579 (Counterclockwise)

Preceding gene: 82701328

Following gene: 82701322

Centisome position: 6.41

GC content: 55.53

Gene sequence:

>1437_bases
TTGCTGAAGAAGACGAGTCGACTTTCCAGTTCCGCATCGTGGAATGATCTGTCCTTTCACCCGTCCTTGACGCTTTCTAC
CACACATATTGCGCGGCTTTACAAGCTGTTTCCACCGGATCGGATCTATACCGATCCGGTGGATTGTTATGCGTATGCAT
ACGATAATACCCGGAAGGTTTTTCCGCCCGATGCCGTGGTATTTCCATTGTCTGCCCTGGAAGTTCAGGCGGCAGTGAAA
TTCGGTAACGAGACGGGGATTCCGCTTGTACCTCGGGGGCGAGGCACCGGCACCTCGGGGGGCAGTCTTCCGGAATCCGG
GGGAATTGCGTTATCGCTGGAACGGATGGTGCGCGTTACCAAAATTGATCCTGCCAATCGCGTACTCGTTGCCGAACCGG
GGGTCATAAACCAGACAGTGCAGGATATAGCAAGAAGGCATGGTTTCTTCTGGCCCCCCGATCCTTCCAGCGCGGCTTTT
TCCAGCATAGGGGGAAACCTTGCGACCTCGGCCGGTGGACCCCGTGCGGTAAAGTACGGAACCACCCGAGACCATGTGCT
GGGAGTGAAGGCAGTTACCGGCAAGGGCCAAGTGATCCGGACGGGCTGCTATACGACCAAGGGCGTCGTGGGATACGACC
TCACCCGACTTCTGATTGGCTCCGAGGGTACACTGGCAGTTATTACCGAAGCCACCCTCAAGCTCACTCCGCTTCCCCAG
GCGTTGGGAGGCGTGACCGCTTATTATAGCGACCTCGCCGGTTGCACAGATGCTATTGTCCGCATCATGACGCTTCCGCA
GACCCCGAGTGCGCTCGAGTTTCTCGATGCGGGGTCCCTCAACCTGATAAGGAGCCGGTTTCCCGGTATGCTTCCTGCCG
ATGCCCACGCCATGCTGATGATTGAAGTGGATGGTACCGAGCATTCGGTCGCAGAGTCACGTAACGCTGTTCTCAGCGCT
TGCGGGGGCAGCGGATTGATTCAGGCCACGCCCACGGCAGATGCTGCAACGCTGTGGCGAGCGAGAAAAACATTATCGCC
GTTATTGAGGGATATTGCGCCAAAAAAAATCAATGAGGACGTCGTCGTGCCGGTTTCGGCTCTGCCAGAGTTTCTGCTGG
GACTCACCAGGTTGAGCGCGAAGCATCGCCTTTCCAACGTCAATTTTGGTCATGCCGGAAACGGCAATATCCATGTCAAT
CTTTTGGTGAATCCGGACTCCACGGGCGAGATGCAGCGGGCGGAAATGTGCCTGAACGAGATATTCGACCTTGTAATCGA
ACTCAACGGCACCTTGTCAGGGGAACACGGCGTCGGGAGCGAGAAAAGAGCATATATCGGAAAAGAGATCGATCCGGTCA
CCATGGAACTCATGAAGGAGATCAAACGCGTGTTTGACCCCAACAACATTTTGAATCCCGGAAAGCTGTTTCCATAG

Upstream 100 bases:

>100_bases
AAGGGAGAGGAAGCGGAGAATGCAGAAGAGCTGGCCAAGCGGGTCGACGGTGTCAAGAAGGTGGTGAAGGTGTTCGAGTA
CATGGAGTAAGCTTCCAGTC

Downstream 100 bases:

>100_bases
ACACGCGTATCCAGCTTGCGAATCGGCAACGGGAATTTTCCGCATCTGTTCAACTGTTCAATTCAAGATTAGCAAAATGC
CTTTGCTAACCCGAAGTTTT

Product: D-lactate dehydrogenase (cytochrome)

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLKKTSRLSSSASWNDLSFHPSLTLSTTHIARLYKLFPPDRIYTDPVDCYAYAYDNTRKVFPPDAVVFPLSALEVQAAVK
FGNETGIPLVPRGRGTGTSGGSLPESGGIALSLERMVRVTKIDPANRVLVAEPGVINQTVQDIARRHGFFWPPDPSSAAF
SSIGGNLATSAGGPRAVKYGTTRDHVLGVKAVTGKGQVIRTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPQ
ALGGVTAYYSDLAGCTDAIVRIMTLPQTPSALEFLDAGSLNLIRSRFPGMLPADAHAMLMIEVDGTEHSVAESRNAVLSA
CGGSGLIQATPTADAATLWRARKTLSPLLRDIAPKKINEDVVVPVSALPEFLLGLTRLSAKHRLSNVNFGHAGNGNIHVN
LLVNPDSTGEMQRAEMCLNEIFDLVIELNGTLSGEHGVGSEKRAYIGKEIDPVTMELMKEIKRVFDPNNILNPGKLFP

Sequences:

>Translated_478_residues
MLKKTSRLSSSASWNDLSFHPSLTLSTTHIARLYKLFPPDRIYTDPVDCYAYAYDNTRKVFPPDAVVFPLSALEVQAAVK
FGNETGIPLVPRGRGTGTSGGSLPESGGIALSLERMVRVTKIDPANRVLVAEPGVINQTVQDIARRHGFFWPPDPSSAAF
SSIGGNLATSAGGPRAVKYGTTRDHVLGVKAVTGKGQVIRTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPQ
ALGGVTAYYSDLAGCTDAIVRIMTLPQTPSALEFLDAGSLNLIRSRFPGMLPADAHAMLMIEVDGTEHSVAESRNAVLSA
CGGSGLIQATPTADAATLWRARKTLSPLLRDIAPKKINEDVVVPVSALPEFLLGLTRLSAKHRLSNVNFGHAGNGNIHVN
LLVNPDSTGEMQRAEMCLNEIFDLVIELNGTLSGEHGVGSEKRAYIGKEIDPVTMELMKEIKRVFDPNNILNPGKLFP
>Mature_478_residues
MLKKTSRLSSSASWNDLSFHPSLTLSTTHIARLYKLFPPDRIYTDPVDCYAYAYDNTRKVFPPDAVVFPLSALEVQAAVK
FGNETGIPLVPRGRGTGTSGGSLPESGGIALSLERMVRVTKIDPANRVLVAEPGVINQTVQDIARRHGFFWPPDPSSAAF
SSIGGNLATSAGGPRAVKYGTTRDHVLGVKAVTGKGQVIRTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPQ
ALGGVTAYYSDLAGCTDAIVRIMTLPQTPSALEFLDAGSLNLIRSRFPGMLPADAHAMLMIEVDGTEHSVAESRNAVLSA
CGGSGLIQATPTADAATLWRARKTLSPLLRDIAPKKINEDVVVPVSALPEFLLGLTRLSAKHRLSNVNFGHAGNGNIHVN
LLVNPDSTGEMQRAEMCLNEIFDLVIELNGTLSGEHGVGSEKRAYIGKEIDPVTMELMKEIKRVFDPNNILNPGKLFP

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=431, Percent_Identity=32.4825986078886, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI37595754, Length=454, Percent_Identity=31.057268722467, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI119964728, Length=474, Percent_Identity=29.957805907173, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4501993, Length=497, Percent_Identity=25.3521126760563, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1789351, Length=444, Percent_Identity=33.7837837837838, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1787977, Length=523, Percent_Identity=28.2982791586998, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI48994914, Length=124, Percent_Identity=36.2903225806452, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994907, Length=262, Percent_Identity=28.2442748091603, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=438, Percent_Identity=31.7351598173516, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71992381, Length=438, Percent_Identity=30.1369863013699, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI71992392, Length=382, Percent_Identity=30.6282722513089, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17534361, Length=425, Percent_Identity=27.5294117647059, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17556096, Length=218, Percent_Identity=27.0642201834862, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320023, Length=480, Percent_Identity=28.3333333333333, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320027, Length=451, Percent_Identity=28.159645232816, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320764, Length=428, Percent_Identity=28.2710280373832, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI18921117, Length=470, Percent_Identity=29.1489361702128, Blast_Score=175, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24639277, Length=470, Percent_Identity=29.1489361702128, Blast_Score=175, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24639275, Length=470, Percent_Identity=29.1489361702128, Blast_Score=175, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24653753, Length=196, Percent_Identity=34.6938775510204, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.28

Molecular weight: Translated: 51123; Mature: 51123

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTSRLSSSASWNDLSFHPSLTLSTTHIARLYKLFPPDRIYTDPVDCYAYAYDNTRKV
CCCCCCCCCCCCCCCCEEECCCEEECHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
FPPDAVVFPLSALEVQAAVKFGNETGIPLVPRGRGTGTSGGSLPESGGIALSLERMVRVT
CCCCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEHHHHHEEE
KIDPANRVLVAEPGVINQTVQDIARRHGFFWPPDPSSAAFSSIGGNLATSAGGPRAVKYG
EECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEC
TTRDHVLGVKAVTGKGQVIRTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPQ
CCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHEEEEECCCCCEEEEEECEEEECCCHH
ALGGVTAYYSDLAGCTDAIVRIMTLPQTPSALEFLDAGSLNLIRSRFPGMLPADAHAMLM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEE
IEVDGTEHSVAESRNAVLSACGGSGLIQATPTADAATLWRARKTLSPLLRDIAPKKINED
EEECCCCHHHHHHHHHHEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCC
VVVPVSALPEFLLGLTRLSAKHRLSNVNFGHAGNGNIHVNLLVNPDSTGEMQRAEMCLNE
EEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
IFDLVIELNGTLSGEHGVGSEKRAYIGKEIDPVTMELMKEIKRVFDPNNILNPGKLFP
HHHHHEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKKTSRLSSSASWNDLSFHPSLTLSTTHIARLYKLFPPDRIYTDPVDCYAYAYDNTRKV
CCCCCCCCCCCCCCCCEEECCCEEECHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
FPPDAVVFPLSALEVQAAVKFGNETGIPLVPRGRGTGTSGGSLPESGGIALSLERMVRVT
CCCCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEHHHHHEEE
KIDPANRVLVAEPGVINQTVQDIARRHGFFWPPDPSSAAFSSIGGNLATSAGGPRAVKYG
EECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEC
TTRDHVLGVKAVTGKGQVIRTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPQ
CCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHEEEEECCCCCEEEEEECEEEECCCHH
ALGGVTAYYSDLAGCTDAIVRIMTLPQTPSALEFLDAGSLNLIRSRFPGMLPADAHAMLM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEE
IEVDGTEHSVAESRNAVLSACGGSGLIQATPTADAATLWRARKTLSPLLRDIAPKKINED
EEECCCCHHHHHHHHHHEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCC
VVVPVSALPEFLLGLTRLSAKHRLSNVNFGHAGNGNIHVNLLVNPDSTGEMQRAEMCLNE
EEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
IFDLVIELNGTLSGEHGVGSEKRAYIGKEIDPVTMELMKEIKRVFDPNNILNPGKLFP
HHHHHEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]