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The map label for this gene is glcD [H]

Identifier: 82701307

GI number: 82701307

Start: 180867

End: 182324

Strand: Direct

Name: glcD [H]

Synonym: Nmul_A0173

Alternate gene names: 82701307

Gene position: 180867-182324 (Clockwise)

Preceding gene: 82701306

Following gene: 82701308

Centisome position: 5.68

GC content: 57.61

Gene sequence:

>1458_bases
ATGAATGACGCTCTCGAACTTGTAAAAAAGCTTCGGACCTTTCTGCCCGCGGAATCGGTGCTGCATGAGACCGAAGACGT
GAGGCCGTACGAGTGCGATGGCCTGTCCGCTTATCGGCAAACGCCCATGATCGTGGCGCTGCCGCGGACTGAAGATGAAG
TGGCGGGAATTTTAAAAATCTGTGATGCACTGAAGATTCCGGTGGTCGCCCGCGGTGCGGGCACTGGTCTCTCCGGCGGT
GCCTTGCCGCATCCGCAGGGAGTGCTGTTGTCGCTGGCGAAGCTGAAAAACATACTGGAGATCGATCCGGCAGCGCGTAC
GGCAAGGGTGCAGCCAGGCGTACGCAACCTTGCGATCAGCGAAGCTGCAGCCCCTTACGGACTGTATTACGCGCCTGATC
CTTCCTCCCAGATTGCCTGCTCCATCGGGGGTAACGTGGCGGAAAATTCCGGAGGTGTGCACTGCCTGAAATACGGACTG
ACAGTGCACAACATCCTCAAACTGCGTGTTTTGACTATTGAAGGCGAGCTTCTCGAAATGGGTGCCGATGGGCTGGACGC
TCCTGGGTACGACCTGCTGGCGCTCATGACGGGCAGCGAAGGCATGCTGGGTATCGTGACCGAGGTGACGGTGAAACTGA
CTCCCCGCCCGGAGAAAGCCCAGGTTGTCCTGGCGTCGTTTGACGACATCCGGGACGCGGGGAATGCAGTGGCGAATGTG
ATCGGCGCCGGCATCATCCCGGCCGGAATGGAAATGATGGACAAGATCACGACCGGGGCAGTGGAAGAGTATGTCCACGC
CGGCTATAACGTCGAAGCGGCTGCGATCCTGTTATGCGAATCCGACGGCGCGGAGGAAGAGGTGGCGGAGGAGATCGAAC
GCATCAACGAGATTATGCGGCAGAGCGGTGCGACGGAAGTCAGGATATCCCGCGACGAAGCCGAACGCGCTCGGTTCTGG
GCGGGGCGGAAGGCGGCTTTTCCTGCCGCAGGCCGGGTTTCCCCTGATTACTATTGCATGGATGGCACCATCCCGCGCAA
GAATCTTGCGAGCGTGCTGACCGGAATCGAAGCGCTTTCTGCCGAATACGGCCTGCGTTGCATGAATGTATTTCATGCGG
GTGACGGCAATCTGCATCCGCTGATTCTTTACGATGCCAATCATCCGGGAGAACTGGAGTTGACAGAGGAATTCGGGGCA
AAAATACTCAGGATGTGCATAGAGGCTGGGGGAACCATTACTGGCGAGCATGGCGTCGGCATTGAAAAAATCAATCAGAT
GTGTTCCCAGTTCAAGACAGGTGAGCTGGAAATGTTTCATGCGGTAAAGGCGGCTTTTGATCCCGCGAGCTTATTGAATC
CCGGAAAAGCCATACCTACCCTGCGGCGCTGTGCCGAGCAGGGGGCGATGCACGTGCATCGGGGCAAAGAGAAATTCCCC
GAATTGCCGAGATTTTGA

Upstream 100 bases:

>100_bases
GGCCCTGGTACGGATGCTGGAAAAACTGGCGAATCATGAGATCGAGACCATCGAAGGCTCGCATGATGAATCATGAGTTG
AATTGGCGGCAATGAACCGT

Downstream 100 bases:

>100_bases
AGTCATGTCCATTTCATATACTCTTCACGGTCTTACAGGCTGACGCAGTATGCAGGAAATCGTTGATCAGCTTGCGGCGA
CCATTCGCGCCGCTGCGGCG

Product: D-lactate dehydrogenase (cytochrome)

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNDALELVKKLRTFLPAESVLHETEDVRPYECDGLSAYRQTPMIVALPRTEDEVAGILKICDALKIPVVARGAGTGLSGG
ALPHPQGVLLSLAKLKNILEIDPAARTARVQPGVRNLAISEAAAPYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGL
TVHNILKLRVLTIEGELLEMGADGLDAPGYDLLALMTGSEGMLGIVTEVTVKLTPRPEKAQVVLASFDDIRDAGNAVANV
IGAGIIPAGMEMMDKITTGAVEEYVHAGYNVEAAAILLCESDGAEEEVAEEIERINEIMRQSGATEVRISRDEAERARFW
AGRKAAFPAAGRVSPDYYCMDGTIPRKNLASVLTGIEALSAEYGLRCMNVFHAGDGNLHPLILYDANHPGELELTEEFGA
KILRMCIEAGGTITGEHGVGIEKINQMCSQFKTGELEMFHAVKAAFDPASLLNPGKAIPTLRRCAEQGAMHVHRGKEKFP
ELPRF

Sequences:

>Translated_485_residues
MNDALELVKKLRTFLPAESVLHETEDVRPYECDGLSAYRQTPMIVALPRTEDEVAGILKICDALKIPVVARGAGTGLSGG
ALPHPQGVLLSLAKLKNILEIDPAARTARVQPGVRNLAISEAAAPYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGL
TVHNILKLRVLTIEGELLEMGADGLDAPGYDLLALMTGSEGMLGIVTEVTVKLTPRPEKAQVVLASFDDIRDAGNAVANV
IGAGIIPAGMEMMDKITTGAVEEYVHAGYNVEAAAILLCESDGAEEEVAEEIERINEIMRQSGATEVRISRDEAERARFW
AGRKAAFPAAGRVSPDYYCMDGTIPRKNLASVLTGIEALSAEYGLRCMNVFHAGDGNLHPLILYDANHPGELELTEEFGA
KILRMCIEAGGTITGEHGVGIEKINQMCSQFKTGELEMFHAVKAAFDPASLLNPGKAIPTLRRCAEQGAMHVHRGKEKFP
ELPRF
>Mature_485_residues
MNDALELVKKLRTFLPAESVLHETEDVRPYECDGLSAYRQTPMIVALPRTEDEVAGILKICDALKIPVVARGAGTGLSGG
ALPHPQGVLLSLAKLKNILEIDPAARTARVQPGVRNLAISEAAAPYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGL
TVHNILKLRVLTIEGELLEMGADGLDAPGYDLLALMTGSEGMLGIVTEVTVKLTPRPEKAQVVLASFDDIRDAGNAVANV
IGAGIIPAGMEMMDKITTGAVEEYVHAGYNVEAAAILLCESDGAEEEVAEEIERINEIMRQSGATEVRISRDEAERARFW
AGRKAAFPAAGRVSPDYYCMDGTIPRKNLASVLTGIEALSAEYGLRCMNVFHAGDGNLHPLILYDANHPGELELTEEFGA
KILRMCIEAGGTITGEHGVGIEKINQMCSQFKTGELEMFHAVKAAFDPASLLNPGKAIPTLRRCAEQGAMHVHRGKEKFP
ELPRF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=423, Percent_Identity=29.5508274231678, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI37595754, Length=446, Percent_Identity=28.0269058295964, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI119964728, Length=448, Percent_Identity=27.6785714285714, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI4501993, Length=248, Percent_Identity=24.5967741935484, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789351, Length=483, Percent_Identity=65.0103519668737, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=504, Percent_Identity=24.2063492063492, Blast_Score=109, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71992373, Length=431, Percent_Identity=29.4663573085847, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI71992381, Length=431, Percent_Identity=28.0742459396752, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI71992392, Length=371, Percent_Identity=28.5714285714286, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17534361, Length=459, Percent_Identity=25.0544662309368, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556096, Length=468, Percent_Identity=22.2222222222222, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320027, Length=433, Percent_Identity=28.175519630485, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320023, Length=479, Percent_Identity=28.8100208768267, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6320764, Length=481, Percent_Identity=25.1559251559252, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI18921117, Length=447, Percent_Identity=26.1744966442953, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639277, Length=447, Percent_Identity=26.1744966442953, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639275, Length=447, Percent_Identity=26.1744966442953, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653753, Length=222, Percent_Identity=25.2252252252252, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51981; Mature: 51981

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDALELVKKLRTFLPAESVLHETEDVRPYECDGLSAYRQTPMIVALPRTEDEVAGILKI
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHHHH
CDALKIPVVARGAGTGLSGGALPHPQGVLLSLAKLKNILEIDPAARTARVQPGVRNLAIS
HHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHH
EAAAPYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNILKLRVLTIEGELLEM
HCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHEEEEEEEEECCHHHHC
GADGLDAPGYDLLALMTGSEGMLGIVTEVTVKLTPRPEKAQVVLASFDDIRDAGNAVANV
CCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHH
IGAGIIPAGMEMMDKITTGAVEEYVHAGYNVEAAAILLCESDGAEEEVAEEIERINEIMR
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
QSGATEVRISRDEAERARFWAGRKAAFPAAGRVSPDYYCMDGTIPRKNLASVLTGIEALS
HCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
AEYGLRCMNVFHAGDGNLHPLILYDANHPGELELTEEFGAKILRMCIEAGGTITGEHGVG
HHHCCEEEEEEECCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCEEECCCCCC
IEKINQMCSQFKTGELEMFHAVKAAFDPASLLNPGKAIPTLRRCAEQGAMHVHRGKEKFP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHCCHHHHCCHHHCC
ELPRF
CCCCC
>Mature Secondary Structure
MNDALELVKKLRTFLPAESVLHETEDVRPYECDGLSAYRQTPMIVALPRTEDEVAGILKI
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHHHH
CDALKIPVVARGAGTGLSGGALPHPQGVLLSLAKLKNILEIDPAARTARVQPGVRNLAIS
HHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHH
EAAAPYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNILKLRVLTIEGELLEM
HCCCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHEEEEEEEEECCHHHHC
GADGLDAPGYDLLALMTGSEGMLGIVTEVTVKLTPRPEKAQVVLASFDDIRDAGNAVANV
CCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHH
IGAGIIPAGMEMMDKITTGAVEEYVHAGYNVEAAAILLCESDGAEEEVAEEIERINEIMR
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
QSGATEVRISRDEAERARFWAGRKAAFPAAGRVSPDYYCMDGTIPRKNLASVLTGIEALS
HCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
AEYGLRCMNVFHAGDGNLHPLILYDANHPGELELTEEFGAKILRMCIEAGGTITGEHGVG
HHHCCEEEEEEECCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCEEECCCCCC
IEKINQMCSQFKTGELEMFHAVKAAFDPASLLNPGKAIPTLRRCAEQGAMHVHRGKEKFP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHCCHHHHCCHHHCC
ELPRF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]