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The map label for this gene is pcm1

Identifier: 82701286

GI number: 82701286

Start: 160502

End: 161212

Strand: Reverse

Name: pcm1

Synonym: Nmul_A0151

Alternate gene names: 82701286

Gene position: 161212-160502 (Counterclockwise)

Preceding gene: 82701287

Following gene: 82701285

Centisome position: 5.06

GC content: 55.98

Gene sequence:

>711_bases
ATGAACTGGCAGTTTGCGACCGCTTTCGGCGCATTGATGTATTTCGGTTGTGTTATTCCGCTGTCAGCTGCGGAATATGA
GGCCTTGCGCCAGAAAATGGTGAGGGAAGTGATTGCCGCATCCGGTAATTCCCCTCCCGGAGCAAGTGTTGTAGCGGCAA
TGGAAAAAGTGGAGCGCCATCGCTTCGTACCTGCCTGGCTCTCCATATTTGCGTATCGCAATCATCCACTCCCGATCGGC
CATGGACAGACGATCTCGCAACCCCTCATCGTTGCGCGGATGACCGAGCTGCTGAAGCTCAAGAAAGACGACAAGGTTCT
TGAAATCGGGACGGGCTCGGGTTATCAGGCTGCTGTCCTGGCGGAGATCGCGAAAACGGTTTATACAATTGAAATCATCG
AACCCCTGGGAAACGAGGCTGCAGGAAGATTGCAATCGCTGGGCTACGACAATGTGAAGACCCGCATAGGGGACGGTTAT
TACGGCTGGCCGGAAGCGGCCCCATTCGACGCCATCCTGGTAACGGCCGCCGCCAGTCATGTGCCCCCTCCTCTGCTCAA
GCAACTGAAGCCCGGCGGACGTATGGTGGTGCCGTTGGGCGCACCTTTCATGACCCAGTACCTGATGCTGGTGGAGAAAC
AGCCGGACGGTTCAGTTACCACCCATCAAATTGTCCCGGTTCGGTTCGTTCCTCTCAGAGGCGGACATTAA

Upstream 100 bases:

>100_bases
GCCTGCGCGATCTTGAACATACCCTGAGTCCAAGCAATGCGCCGCAACGAGTAAGGCGGCGCATTTCAGCAAGTTGCCGC
AAATTTGCTTTAATGAACTG

Downstream 100 bases:

>100_bases
TCATCATGTCCTTGCCACGTCCGCCCTTTATTGCCATCGGACTGCTCTCCGCCTGCGTACTGGCATATGAGGTATTGCTC
ACGCGCTTGTTCAGCATCGT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 1; Protein L-isoaspartyl methyltransferase 1; Protein-beta-aspartate methyltransferase 1; PIMT 1

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNWQFATAFGALMYFGCVIPLSAAEYEALRQKMVREVIAASGNSPPGASVVAAMEKVERHRFVPAWLSIFAYRNHPLPIG
HGQTISQPLIVARMTELLKLKKDDKVLEIGTGSGYQAAVLAEIAKTVYTIEIIEPLGNEAAGRLQSLGYDNVKTRIGDGY
YGWPEAAPFDAILVTAAASHVPPPLLKQLKPGGRMVVPLGAPFMTQYLMLVEKQPDGSVTTHQIVPVRFVPLRGGH

Sequences:

>Translated_236_residues
MNWQFATAFGALMYFGCVIPLSAAEYEALRQKMVREVIAASGNSPPGASVVAAMEKVERHRFVPAWLSIFAYRNHPLPIG
HGQTISQPLIVARMTELLKLKKDDKVLEIGTGSGYQAAVLAEIAKTVYTIEIIEPLGNEAAGRLQSLGYDNVKTRIGDGY
YGWPEAAPFDAILVTAAASHVPPPLLKQLKPGGRMVVPLGAPFMTQYLMLVEKQPDGSVTTHQIVPVRFVPLRGGH
>Mature_236_residues
MNWQFATAFGALMYFGCVIPLSAAEYEALRQKMVREVIAASGNSPPGASVVAAMEKVERHRFVPAWLSIFAYRNHPLPIG
HGQTISQPLIVARMTELLKLKKDDKVLEIGTGSGYQAAVLAEIAKTVYTIEIIEPLGNEAAGRLQSLGYDNVKTRIGDGY
YGWPEAAPFDAILVTAAASHVPPPLLKQLKPGGRMVVPLGAPFMTQYLMLVEKQPDGSVTTHQIVPVRFVPLRGGH

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=171, Percent_Identity=36.8421052631579, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1789100, Length=188, Percent_Identity=47.8723404255319, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71983477, Length=190, Percent_Identity=32.1052631578947, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193207222, Length=188, Percent_Identity=30.8510638297872, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17981723, Length=227, Percent_Identity=31.2775330396476, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT1_NITMU (Q2YCR1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_410852.1
- ProteinModelPortal:   Q2YCR1
- SMR:   Q2YCR1
- STRING:   Q2YCR1
- GeneID:   3785483
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0151
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   GYKATIS
- PhylomeDB:   Q2YCR1
- BioCyc:   NMUL323848:NMUL_A0151-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 25648; Mature: 25648

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 86-86

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWQFATAFGALMYFGCVIPLSAAEYEALRQKMVREVIAASGNSPPGASVVAAMEKVERH
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RFVPAWLSIFAYRNHPLPIGHGQTISQPLIVARMTELLKLKKDDKVLEIGTGSGYQAAVL
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
AEIAKTVYTIEIIEPLGNEAAGRLQSLGYDNVKTRIGDGYYGWPEAAPFDAILVTAAASH
HHHHHHHHHHEEHHHCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
VPPPLLKQLKPGGRMVVPLGAPFMTQYLMLVEKQPDGSVTTHQIVPVRFVPLRGGH
CCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCC
>Mature Secondary Structure
MNWQFATAFGALMYFGCVIPLSAAEYEALRQKMVREVIAASGNSPPGASVVAAMEKVERH
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RFVPAWLSIFAYRNHPLPIGHGQTISQPLIVARMTELLKLKKDDKVLEIGTGSGYQAAVL
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
AEIAKTVYTIEIIEPLGNEAAGRLQSLGYDNVKTRIGDGYYGWPEAAPFDAILVTAAASH
HHHHHHHHHHEEHHHCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
VPPPLLKQLKPGGRMVVPLGAPFMTQYLMLVEKQPDGSVTTHQIVPVRFVPLRGGH
CCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA