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The map label for this gene is metK [H]

Identifier: 82701171

GI number: 82701171

Start: 35230

End: 36420

Strand: Reverse

Name: metK [H]

Synonym: Nmul_A0036

Alternate gene names: 82701171

Gene position: 36420-35230 (Counterclockwise)

Preceding gene: 82701172

Following gene: 82701166

Centisome position: 1.14

GC content: 55.84

Gene sequence:

>1191_bases
ATGATGAAACAGAATTTCATGTTCACCTCCGAATCTGTAACCGAAGGGCATCCGGATAAGCTTTGCGATCAAGTGAGCGA
TGCGTTGGTCGACCGCTTTCTGCAGCAAGACCCCTTTTCGAAGGTGATAGCGGAGTGCGCAGTATCCACGGGAATACTTT
TTATCGCAGCCCGCTTCGCTTCGTCGGCTTCTGTCGACATCCCCGAGGTTGCGCGCCGGGTTATACACCAGCTTGGCTAC
GAGCAAGCGGATTTCAATGCAAGAGATTGCACGATCATGACGAGCCTGAGCGAACTGCCGGCACCGTCTTATCCGTTCAT
AGATGAGAGGGAAATGAATGAGGAAGAGCTGGAATCCGTTACTGCCAAGAACCAGGCCAATGTATTCGGCTTTGCCTGTA
ATCAGAGCTCGGCCTTTATGCCGCTGCCCATCTCGCTTTCGCATAAACTGGCGCGTCGGCTTACGGCCGCACGTTTTCAG
AAGCAGATCCCCTATCTTGCGCCCGATGGAAAAACGCAGGTAGGGGTGGAATATCGCGAAGGCAGACCGTATCGCATTCA
TTCCATCACCATCATTGCCGCTCAGCAGAAGACCGCCATGCGCGGCCTCGCACCGCTGCGCGATGATCTCAACGCGCATG
TGATTGAGCCGGTTTTCGCAACGGAGACGCTGCGGCCCGACTCCCGCACCCATATTTTCATCAATCCGGAAGGGCTGGTC
GCCGACGGAGGACCGTCGCTACATTCCGGCTTGACTGGCCGCAAGAACGCAGTGGATACATACGGTGAATATTCCCGGCA
TTCGGAATCCGCGCTTTCCGGCAAGGACCCTTCTCGTATCGATCGCGTGGGAGCGTATGCAGCGCGCTACGCGGCCAAGA
ATGTAGTCGCCGCCGGCCTCGCCCAGGCATGCGAGGTACATCTGGCGTATTCGATAGGAATATCGAGGCCGGTAAGCGTG
CAGGCGGATACTTTTGGTACAGGGAGCGTTCCTGATACCGAAATAACGGCGCGTATTCTCGACCAGTTTGATTTCCGCCC
TGCCGGGATCATCCGCGCCTTTAATTTGCGCTATCAGCCTCAACTATTTCGCGGTTTGTTTTACCGCAAGCTTGCGGTTT
ACGGGCAGGTGGGACGGATGGATATCGGATTGCCGTGGGAGAACACGGACAAGGCTGCATTGCTGCGCTAG

Upstream 100 bases:

>100_bases
TACTGCCTTTTTTCCTGAATGAAGCAACCAAAGAGACTGCCTTCAAAGGCAAGCGACGTAAAGAGCCGCTATCTCTACCA
CCAGCGCCGCAGGAGTATGG

Downstream 100 bases:

>100_bases
GCTGGATATTGGGTGGGTCAACGCATCTCGAGCGATATGCCCAGCTCAGATCAGTGCTGCGACCAGAGCCACAGCCGACC
GTTCCAATGCAGTTTCCACG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MMKQNFMFTSESVTEGHPDKLCDQVSDALVDRFLQQDPFSKVIAECAVSTGILFIAARFASSASVDIPEVARRVIHQLGY
EQADFNARDCTIMTSLSELPAPSYPFIDEREMNEEELESVTAKNQANVFGFACNQSSAFMPLPISLSHKLARRLTAARFQ
KQIPYLAPDGKTQVGVEYREGRPYRIHSITIIAAQQKTAMRGLAPLRDDLNAHVIEPVFATETLRPDSRTHIFINPEGLV
ADGGPSLHSGLTGRKNAVDTYGEYSRHSESALSGKDPSRIDRVGAYAARYAAKNVVAAGLAQACEVHLAYSIGISRPVSV
QADTFGTGSVPDTEITARILDQFDFRPAGIIRAFNLRYQPQLFRGLFYRKLAVYGQVGRMDIGLPWENTDKAALLR

Sequences:

>Translated_396_residues
MMKQNFMFTSESVTEGHPDKLCDQVSDALVDRFLQQDPFSKVIAECAVSTGILFIAARFASSASVDIPEVARRVIHQLGY
EQADFNARDCTIMTSLSELPAPSYPFIDEREMNEEELESVTAKNQANVFGFACNQSSAFMPLPISLSHKLARRLTAARFQ
KQIPYLAPDGKTQVGVEYREGRPYRIHSITIIAAQQKTAMRGLAPLRDDLNAHVIEPVFATETLRPDSRTHIFINPEGLV
ADGGPSLHSGLTGRKNAVDTYGEYSRHSESALSGKDPSRIDRVGAYAARYAAKNVVAAGLAQACEVHLAYSIGISRPVSV
QADTFGTGSVPDTEITARILDQFDFRPAGIIRAFNLRYQPQLFRGLFYRKLAVYGQVGRMDIGLPWENTDKAALLR
>Mature_396_residues
MMKQNFMFTSESVTEGHPDKLCDQVSDALVDRFLQQDPFSKVIAECAVSTGILFIAARFASSASVDIPEVARRVIHQLGY
EQADFNARDCTIMTSLSELPAPSYPFIDEREMNEEELESVTAKNQANVFGFACNQSSAFMPLPISLSHKLARRLTAARFQ
KQIPYLAPDGKTQVGVEYREGRPYRIHSITIIAAQQKTAMRGLAPLRDDLNAHVIEPVFATETLRPDSRTHIFINPEGLV
ADGGPSLHSGLTGRKNAVDTYGEYSRHSESALSGKDPSRIDRVGAYAARYAAKNVVAAGLAQACEVHLAYSIGISRPVSV
QADTFGTGSVPDTEITARILDQFDFRPAGIIRAFNLRYQPQLFRGLFYRKLAVYGQVGRMDIGLPWENTDKAALLR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=38.3033419023136, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=38.9175257731959, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI1789311, Length=392, Percent_Identity=40.3061224489796, Blast_Score=305, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI25145633, Length=397, Percent_Identity=38.0352644836272, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=38.563829787234, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17538494, Length=393, Percent_Identity=37.9134860050891, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17551082, Length=393, Percent_Identity=38.676844783715, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=35.2941176470588, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI25145635, Length=346, Percent_Identity=34.6820809248555, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32565911, Length=325, Percent_Identity=35.3846153846154, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI32565903, Length=307, Percent_Identity=35.8306188925081, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=46.9879518072289, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320710, Length=359, Percent_Identity=40.6685236768802, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6323209, Length=393, Percent_Identity=38.4223918575064, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24580527, Length=386, Percent_Identity=38.0829015544041, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24580525, Length=386, Percent_Identity=38.0829015544041, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI45552159, Length=388, Percent_Identity=37.6288659793814, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24580529, Length=388, Percent_Identity=37.6288659793814, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24580537, Length=388, Percent_Identity=37.6288659793814, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24580533, Length=388, Percent_Identity=37.6288659793814, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24580535, Length=388, Percent_Identity=37.6288659793814, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24580531, Length=388, Percent_Identity=37.6288659793814, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24580541, Length=387, Percent_Identity=35.9173126614987, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=37.6, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43723; Mature: 43723

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKQNFMFTSESVTEGHPDKLCDQVSDALVDRFLQQDPFSKVIAECAVSTGILFIAARFA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
SSASVDIPEVARRVIHQLGYEQADFNARDCTIMTSLSELPAPSYPFIDEREMNEEELESV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
TAKNQANVFGFACNQSSAFMPLPISLSHKLARRLTAARFQKQIPYLAPDGKTQVGVEYRE
HHCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECEEECC
GRPYRIHSITIIAAQQKTAMRGLAPLRDDLNAHVIEPVFATETLRPDSRTHIFINPEGLV
CCCEEEEEEEEEEECHHHHHHCCHHHHHCCCCEEECHHHHHHCCCCCCCEEEEECCCCEE
ADGGPSLHSGLTGRKNAVDTYGEYSRHSESALSGKDPSRIDRVGAYAARYAAKNVVAAGL
ECCCCHHHCCCCCCCCCHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQACEVHLAYSIGISRPVSVQADTFGTGSVPDTEITARILDQFDFRPAGIIRAFNLRYQP
HHHHHEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEECCCCCH
QLFRGLFYRKLAVYGQVGRMDIGLPWENTDKAALLR
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHCCC
>Mature Secondary Structure
MMKQNFMFTSESVTEGHPDKLCDQVSDALVDRFLQQDPFSKVIAECAVSTGILFIAARFA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
SSASVDIPEVARRVIHQLGYEQADFNARDCTIMTSLSELPAPSYPFIDEREMNEEELESV
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
TAKNQANVFGFACNQSSAFMPLPISLSHKLARRLTAARFQKQIPYLAPDGKTQVGVEYRE
HHCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECEEECC
GRPYRIHSITIIAAQQKTAMRGLAPLRDDLNAHVIEPVFATETLRPDSRTHIFINPEGLV
CCCEEEEEEEEEEECHHHHHHCCHHHHHCCCCEEECHHHHHHCCCCCCCEEEEECCCCEE
ADGGPSLHSGLTGRKNAVDTYGEYSRHSESALSGKDPSRIDRVGAYAARYAAKNVVAAGL
ECCCCHHHCCCCCCCCCHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQACEVHLAYSIGISRPVSVQADTFGTGSVPDTEITARILDQFDFRPAGIIRAFNLRYQP
HHHHHEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEECCCCCH
QLFRGLFYRKLAVYGQVGRMDIGLPWENTDKAALLR
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA