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The map label for this gene is mnmG

Identifier: 162139859

GI number: 162139859

Start: 3175097

End: 3176995

Strand: Reverse

Name: mnmG

Synonym: Nmul_A2770

Alternate gene names: 162139859

Gene position: 3176995-3175097 (Counterclockwise)

Preceding gene: 82703886

Following gene: 82703882

Centisome position: 99.77

GC content: 56.19

Gene sequence:

>1899_bases
ATGACTGTTCCTGAAATATTCGATGTAATAGTTATAGGTGGTGGCCACGCCGGTACCGAAGCGGCGCTCGCTGCCGCTCG
CATTGGGCAAAAGACACTATTGCTATCACACAACATCGAAACCCTGGGGCAGATGTCGTGCAACCCTTCCATAGGCGGGA
TCGGCAAAGGGCATCTGGTCAAAGAAATCGATGCGCTTGGGGGCGTGATGGCAGAGGCAGCGGATGAGGCGGGCATACAG
TTTCGCATCCTCAACTCGCGCAAAGGACCTGCGGTACGCGCAACACGCGCCCAGGCTGATCGGGTCCTCTACCGCCAAGC
GATCCGGCAGCGGTTGGAAAGTCAGCCGAATCTCTGGTTGTTTCAGCAAGGAGTGGACGATCTCGTGCTTGAGGGTGAGC
GGGTGGCCGGGGTTGTCACACAGCTTGGAATCCGCTTTAACGCGCGTGCTGTAATTCTTACCGCTGGAACCTTCCTGGCA
GGCCTGGCTCACGTAGGCTCGGCAAATTTTCAAGCCGGGCGGGCGGGGGACCTTCCATCCAATAGTCTTGCGGGACGCCT
GCGCGAATTGAAGCTCCCTGTCGGGCGGTTGAAAACAGGAACCCCACCGCGGATAGATGGCCGTACCATCGATTATTCCG
CTGTTGCCGCTCAACCCGGCGACGCGCCTGTGCCCGTTTTTTCCTTTATGGGCAATGCTGCTTCCCACCCGAAGCAGGTG
TCTTGCTGGATCACGCATACCAACAGTCAGACGCACGATATTATTCGCAGCGGCCTGGATCGCTCCCCTCTATTCTCCGG
TGCGATCGAGGGTATCGGACCTCGTTATTGTCCCTCTATCGAGGACAAGGTCGTGCGCTTTTCCGCCAAGGAATCGCATC
AGATTTTTCTCGAACCGGAGGGTCTCACGACCCACGAGGTGTATCCCAACGGAATCTCCACGAGTCTCCCCTTCGATATG
CAGGTGAAACTGGTGCGCTCAATCAAAGGATTGGAGAACGCCCATATCACCCGACCCGGTTATGCGATCGAATATGATTA
TTTTGATCCGCGTGCCCTGAAAAGCTCGCTTGAAACCAGGATGATCGAAGGCCTGTTTTTCGCGGGACAGATCAATGGCA
CCACGGGTTATGAAGAGGCCGCGGCGCAGGGGTTGCTTGCGGGAATCAACGCTGCGCTAAAAACGCAGGACAGGGAACCG
TGGTGTCCGCGCCGGGATGAAGCCTATTTGGGCGTCCTGGTGGACGATCTCATTACCCGAGGTGTTACTGAACCCTATCG
CATGTTCACCAGCCGTGCGGAATACCGGTTGCAATTGCGGGAGGATAACGCCGATCTCCGCCTCACCGAAGTCGGACGCA
GGCTAGGGGTGGTGAATGACGCGCGCTGGGTGGCGTTCAATATCAAACTAGAGGCTATCGCTCAAGAGCAAAACCGGCTG
AAGACCACGTGGCTCAGCCCGAAGAGTCTTTCCGAAACCGATGCATTACGTGTTATCGGGAAAAACATCGAGCATGAGTG
TTCTCTGCATGACTTGTTGCGCCGGCCCAATGTGTCGTACGCCGAACTCATGACGCTGCCCGGTGCAGGCGAACCCGTTG
TCGATCTTGCGGCGGCGGAGCAGGTCGAAATCCAGGCGAAGTATCAGGGATACATCGAGCGCCAGAAAGATGAAGTGGCG
CGAAATGCTTATTATGAAGACATTCGCCTGCCGCAGGACCTGGATTACAAAACCGTGCGCGGTCTTTCGAATGAAGTTCA
GCAGAAACTTAATCAGTTCAAGCCTGAAACGGCAGGACAGGCATCCCGTATCTCCGGAATCACGCCCGCCGCAATTTCGC
TATTGCTGGTCCATGTCAAGCGCGGTTTTTCCAGCGCGAATACGAAGAAAAGCGCATGA

Upstream 100 bases:

>100_bases
TTCTTCACCCAAGACCTCTTTATGATTCTTACATGGCTCCACGTTTTGCCGTAAAATGGGCACCACTCTCAATCGAATTC
ATTTTTGCCCGATTGCTTCC

Downstream 100 bases:

>100_bases
ATCCGGCTACACAACTGGCTGACGGAATCGCAACACTGGGACTATCTATTACCGAATCAAATCAGGCACGCCTCCTGCAA
TATCTGGCGCTCATCCAGAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MTVPEIFDVIVIGGGHAGTEAALAAARIGQKTLLLSHNIETLGQMSCNPSIGGIGKGHLVKEIDALGGVMAEAADEAGIQ
FRILNSRKGPAVRATRAQADRVLYRQAIRQRLESQPNLWLFQQGVDDLVLEGERVAGVVTQLGIRFNARAVILTAGTFLA
GLAHVGSANFQAGRAGDLPSNSLAGRLRELKLPVGRLKTGTPPRIDGRTIDYSAVAAQPGDAPVPVFSFMGNAASHPKQV
SCWITHTNSQTHDIIRSGLDRSPLFSGAIEGIGPRYCPSIEDKVVRFSAKESHQIFLEPEGLTTHEVYPNGISTSLPFDM
QVKLVRSIKGLENAHITRPGYAIEYDYFDPRALKSSLETRMIEGLFFAGQINGTTGYEEAAAQGLLAGINAALKTQDREP
WCPRRDEAYLGVLVDDLITRGVTEPYRMFTSRAEYRLQLREDNADLRLTEVGRRLGVVNDARWVAFNIKLEAIAQEQNRL
KTTWLSPKSLSETDALRVIGKNIEHECSLHDLLRRPNVSYAELMTLPGAGEPVVDLAAAEQVEIQAKYQGYIERQKDEVA
RNAYYEDIRLPQDLDYKTVRGLSNEVQQKLNQFKPETAGQASRISGITPAAISLLLVHVKRGFSSANTKKSA

Sequences:

>Translated_632_residues
MTVPEIFDVIVIGGGHAGTEAALAAARIGQKTLLLSHNIETLGQMSCNPSIGGIGKGHLVKEIDALGGVMAEAADEAGIQ
FRILNSRKGPAVRATRAQADRVLYRQAIRQRLESQPNLWLFQQGVDDLVLEGERVAGVVTQLGIRFNARAVILTAGTFLA
GLAHVGSANFQAGRAGDLPSNSLAGRLRELKLPVGRLKTGTPPRIDGRTIDYSAVAAQPGDAPVPVFSFMGNAASHPKQV
SCWITHTNSQTHDIIRSGLDRSPLFSGAIEGIGPRYCPSIEDKVVRFSAKESHQIFLEPEGLTTHEVYPNGISTSLPFDM
QVKLVRSIKGLENAHITRPGYAIEYDYFDPRALKSSLETRMIEGLFFAGQINGTTGYEEAAAQGLLAGINAALKTQDREP
WCPRRDEAYLGVLVDDLITRGVTEPYRMFTSRAEYRLQLREDNADLRLTEVGRRLGVVNDARWVAFNIKLEAIAQEQNRL
KTTWLSPKSLSETDALRVIGKNIEHECSLHDLLRRPNVSYAELMTLPGAGEPVVDLAAAEQVEIQAKYQGYIERQKDEVA
RNAYYEDIRLPQDLDYKTVRGLSNEVQQKLNQFKPETAGQASRISGITPAAISLLLVHVKRGFSSANTKKSA
>Mature_631_residues
TVPEIFDVIVIGGGHAGTEAALAAARIGQKTLLLSHNIETLGQMSCNPSIGGIGKGHLVKEIDALGGVMAEAADEAGIQF
RILNSRKGPAVRATRAQADRVLYRQAIRQRLESQPNLWLFQQGVDDLVLEGERVAGVVTQLGIRFNARAVILTAGTFLAG
LAHVGSANFQAGRAGDLPSNSLAGRLRELKLPVGRLKTGTPPRIDGRTIDYSAVAAQPGDAPVPVFSFMGNAASHPKQVS
CWITHTNSQTHDIIRSGLDRSPLFSGAIEGIGPRYCPSIEDKVVRFSAKESHQIFLEPEGLTTHEVYPNGISTSLPFDMQ
VKLVRSIKGLENAHITRPGYAIEYDYFDPRALKSSLETRMIEGLFFAGQINGTTGYEEAAAQGLLAGINAALKTQDREPW
CPRRDEAYLGVLVDDLITRGVTEPYRMFTSRAEYRLQLREDNADLRLTEVGRRLGVVNDARWVAFNIKLEAIAQEQNRLK
TTWLSPKSLSETDALRVIGKNIEHECSLHDLLRRPNVSYAELMTLPGAGEPVVDLAAAEQVEIQAKYQGYIERQKDEVAR
NAYYEDIRLPQDLDYKTVRGLSNEVQQKLNQFKPETAGQASRISGITPAAISLLLVHVKRGFSSANTKKSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=642, Percent_Identity=46.2616822429907, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI19882217, Length=667, Percent_Identity=44.527736131934, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI183227703, Length=682, Percent_Identity=43.5483870967742, Blast_Score=477, Evalue=1e-134,
Organism=Escherichia coli, GI2367273, Length=621, Percent_Identity=67.3107890499195, Blast_Score=874, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=625, Percent_Identity=40.8, Blast_Score=460, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6321202, Length=623, Percent_Identity=45.5858747993579, Blast_Score=510, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24658174, Length=644, Percent_Identity=45.8074534161491, Blast_Score=503, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_NITMU (Q2Y5B4)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413449.2
- ProteinModelPortal:   Q2Y5B4
- SMR:   Q2Y5B4
- STRING:   Q2Y5B4
- GeneID:   3785365
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2770
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- PhylomeDB:   Q2Y5B4
- ProtClustDB:   PRK05192
- BioCyc:   NMUL323848:NMUL_A2770-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69164; Mature: 69032

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 125-125 BINDING 180-180 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPEIFDVIVIGGGHAGTEAALAAARIGQKTLLLSHNIETLGQMSCNPSIGGIGKGHLV
CCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCCCCCCCCHHH
KEIDALGGVMAEAADEAGIQFRILNSRKGPAVRATRAQADRVLYRQAIRQRLESQPNLWL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
FQQGVDDLVLEGERVAGVVTQLGIRFNARAVILTAGTFLAGLAHVGSANFQAGRAGDLPS
ECCCCHHHHHCCHHHHHHHHHHCCEECCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC
NSLAGRLRELKLPVGRLKTGTPPRIDGRTIDYSAVAAQPGDAPVPVFSFMGNAASHPKQV
HHHHHHHHHHCCCHHHCCCCCCCCCCCCEECCCEEECCCCCCCCHHHHHCCCCCCCCCEE
SCWITHTNSQTHDIIRSGLDRSPLFSGAIEGIGPRYCPSIEDKVVRFSAKESHQIFLEPE
EEEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHEEEECCCCCEEEECCC
GLTTHEVYPNGISTSLPFDMQVKLVRSIKGLENAHITRPGYAIEYDYFDPRALKSSLETR
CCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
MIEGLFFAGQINGTTGYEEAAAQGLLAGINAALKTQDREPWCPRRDEAYLGVLVDDLITR
HHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
GVTEPYRMFTSRAEYRLQLREDNADLRLTEVGRRLGVVNDARWVAFNIKLEAIAQEQNRL
CCCHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHH
KTTWLSPKSLSETDALRVIGKNIEHECSLHDLLRRPNVSYAELMTLPGAGEPVVDLAAAE
EEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHEECCCCCCCHHHHHCCC
QVEIQAKYQGYIERQKDEVARNAYYEDIRLPQDLDYKTVRGLSNEVQQKLNQFKPETAGQ
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
ASRISGITPAAISLLLVHVKRGFSSANTKKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TVPEIFDVIVIGGGHAGTEAALAAARIGQKTLLLSHNIETLGQMSCNPSIGGIGKGHLV
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCCCCCCCCHHH
KEIDALGGVMAEAADEAGIQFRILNSRKGPAVRATRAQADRVLYRQAIRQRLESQPNLWL
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
FQQGVDDLVLEGERVAGVVTQLGIRFNARAVILTAGTFLAGLAHVGSANFQAGRAGDLPS
ECCCCHHHHHCCHHHHHHHHHHCCEECCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC
NSLAGRLRELKLPVGRLKTGTPPRIDGRTIDYSAVAAQPGDAPVPVFSFMGNAASHPKQV
HHHHHHHHHHCCCHHHCCCCCCCCCCCCEECCCEEECCCCCCCCHHHHHCCCCCCCCCEE
SCWITHTNSQTHDIIRSGLDRSPLFSGAIEGIGPRYCPSIEDKVVRFSAKESHQIFLEPE
EEEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHEEEECCCCCEEEECCC
GLTTHEVYPNGISTSLPFDMQVKLVRSIKGLENAHITRPGYAIEYDYFDPRALKSSLETR
CCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
MIEGLFFAGQINGTTGYEEAAAQGLLAGINAALKTQDREPWCPRRDEAYLGVLVDDLITR
HHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
GVTEPYRMFTSRAEYRLQLREDNADLRLTEVGRRLGVVNDARWVAFNIKLEAIAQEQNRL
CCCHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHH
KTTWLSPKSLSETDALRVIGKNIEHECSLHDLLRRPNVSYAELMTLPGAGEPVVDLAAAE
EEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHEECCCCCCCHHHHHCCC
QVEIQAKYQGYIERQKDEVARNAYYEDIRLPQDLDYKTVRGLSNEVQQKLNQFKPETAGQ
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
ASRISGITPAAISLLLVHVKRGFSSANTKKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA