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The map label for this gene is hpaH [H]

Identifier: 82546702

GI number: 82546702

Start: 4462765

End: 4463415

Strand: Reverse

Name: hpaH [H]

Synonym: SBO_4408

Alternate gene names: 82546702

Gene position: 4463415-4462765 (Counterclockwise)

Preceding gene: 82546703

Following gene: 82546701

Centisome position: 98.75

GC content: 58.22

Gene sequence:

>651_bases
ATGTTCGATAAACACACCCACACCCTGATCGCCCAGCGTCTGGATCAGGCAGAAAAACAGCGCGAACAGATCCGCGCGAT
CTCGCTGGATTACCCAGAGATCACCATCGAAGACGCTTACGCGGTGCAGCGCGAATGGGTTCGCCTGAAAATCGCCGAAG
GCCGTACGCTGAAAGGCCACAAAATCGGTCTGACCTCAAAAGCAATGCAGGCCAGCTCGCAGATCAGCGAACCAGATTAC
GGTGCCCTGCTGGATGACATGTTCTTCCACGATGGCAGCGATATCCCGACCGATCGCTTTATCGTGCCGCGCATTGAAGT
GGAGCTGGCTTTCGTGCTGGCAAAACCGCTGCGTGGGCCAAACTGCACGCTGTTCGACGTCTACAACGCCACGGACTACG
TGATCCCGGCGCTGGAGCTGATCGACGCTCGCTGCCACAACATCGATCCGGAAACCCAGCGCCCGCGCAAAGTGTTCGAC
ACCATTTCTGATAACGCCGCCAACGCCGGGGTGATCCTCGGTGGTCGTCCGATCAAACCAGACGAACTGGATCTGCGTTG
GATCTCCGCCCTGATGTATCGCAATGGCGTGATTGAAGAAACCGGCGTCGCCGCTGGCGTGCTGAATCATCCGGCAAACG
GCGTGGCCTAG

Upstream 100 bases:

>100_bases
GCCCGGTGGCGCTGCGCTTACCGGGCCTACAAAACCCCAAACCGTACACCGTAGGCCGGATAAGGCGCAGCCGCATCCGG
CAATGCCACAGGATATCGCT

Downstream 100 bases:

>100_bases
CTGGCGAACAAACTCGCACCGTATGACGTACAACTGGAAGCCGGGCAAATCATTCTCGGTGGTTCGTTCACCCGCCCGGT
TCCGGCGCGTAAGGGCGACA

Product: 2-oxo-hept-3-ene-1,7-dioate hydratase

Products: NA

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDY
GALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVA

Sequences:

>Translated_216_residues
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDY
GALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVA
>Mature_216_residues
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDY
GALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVA

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=210, Percent_Identity=34.2857142857143, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.-

Molecular weight: Translated: 24136; Mature: 24136

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGH
CCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHEEEEEECCCCEECCC
KIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGP
EECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHEEEEEECCCCCCC
NCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFDTISDNAANAGVILGGRPIKP
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCC
DELDLRWISALMYRNGVIEETGVAAGVLNHPANGVA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGH
CCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHEEEEEECCCCEECCC
KIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGP
EECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHEEEEEECCCCCCC
NCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFDTISDNAANAGVILGGRPIKP
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCC
DELDLRWISALMYRNGVIEETGVAAGVLNHPANGVA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]