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The map label for this gene is yjiO [H]

Identifier: 82546684

GI number: 82546684

Start: 4441228

End: 4442460

Strand: Reverse

Name: yjiO [H]

Synonym: SBO_4389

Alternate gene names: 82546684

Gene position: 4442460-4441228 (Counterclockwise)

Preceding gene: 82546689

Following gene: 82546683

Centisome position: 98.29

GC content: 54.5

Gene sequence:

>1233_bases
ATGCCACGTTTTTTTGCCCGCCATGCCGCCACGCTGTTTTTCCCGATGGCGTTGATTTTGTATGACTTTGCCGCGTACCT
GTCGACGGATCTGATCCAGCCGGGGATCATTAATGTGGTCCGTGATTTTAATGCCGATGTCAGTCTGGCCCCGGCAGCCG
TCAGTCTCTATCTCGCGGGCGGTATGGCGTTACAGTGGCTGCTGGGGCCGCTTTCCGACAGGATTGGCCGCAGGCCGGTG
CTGATTACCGGGGCGCTAATATTTACCCTTGCCTGCGCTGCGACAATGTTCACAACGTCTATGACACAGTTTCTTATCGC
GCGTGCAATTCAGGGCACCAGTATCTGTTTTATTGCCACCGTTGGTTATGTCACGGTGCAGGAGGCGTTCGGCCAGACAA
AAGGGATCAAGTTGATGGCGATTATCACCTCCATCGTACTGATTGCGCCGATTATTGGCCCGCTTTCCGGCGCAGCTCTG
ATGCACTTTGTGCACTGGAAAGTCCTTTTTGCCATCATTGCAGTTATGGGTTTTATCTCATTTGTTGGCTTACTGCTGGC
GATGCCAGAGACGGTGAAGCGCGGCGCGGTTCCATTTAGCGCCAAAAGCGTCTTTCGCGATTTTCGTAATGTCTTTTGCA
ATCGGCTGTTCCTCTTTGGCGCAGCAACCATCTCTTTAAGCTATATCCCAATGATGAGTTGGGTGGCTGTCTCGCCGGTG
ATCCTGATCGACGCGGGCAGCTTAACAACTTCGCAGTTCGCCTGGACACAGGTTCCGGTGTTCGGCGCGGTGATTGTCGC
GAATGCCATCGTGGCGCGTTTCGTTAAAGATCCGACCGAACCGCGCTTTATCTGGCGTGCTGTACCCATTCAACTGGTCG
GCCTCGCGCTGTTGATTATCGGCAATCTGCTGTCACCGCACGTCTGGCTGTGGTCGGTGCTGGGCACCAGTTTGTATGCT
TTCGGGATTGGTTTGATCTTCCCGACCTTATTCCGCTTTACGCTTTTTTCCAATAACTTACCGAAAGGGACCGTCTCCGC
ATCGCTGAATATGGTGATCCTGATGGTGATGTCTGTCTCGGTCGAAATCGGCCGCTGGCTGTGGTTTAACGGCGGTCGCT
TGCCGTTTCATCTGTTAGCCGTCGTGGCGGGCGTTATCGTCGTTTTCACCCTGGCAGGATTGCTTAATCGCGTGCGCCAA
CATCAGGCTGCCGAGCTGGCGGAGGAGCGGTGA

Upstream 100 bases:

>100_bases
TTTGCAGTCTCGTTGCCCTTCTCGCCTGCCTTTCGTCATTACCGACGGTATTGAATTTCGTTTTCCCCGTTGGGGTTCTC
CGAACAAGGAGTTGTTTGTT

Downstream 100 bases:

>100_bases
TTTTTGCGCGATCCTGCCGTCAGGCTCTATTCTTAACGTTATGAATAAACTCATTGAACTCAGACGCGCCAAAATGTTGG
CGCTCTCTTTACTGCTTATC

Product: transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPV
LITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPV
ILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYA
FGIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQ
HQAAELAEER

Sequences:

>Translated_410_residues
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPV
LITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPV
ILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYA
FGIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQ
HQAAELAEER
>Mature_409_residues
PRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVL
ITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALM
HFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPVI
LIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAF
GIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQH
QAAELAEER

Specific function: Confers resistance to acriflavine, chloramphenicol, norfloxacin, ethidium bromide and TPP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790794, Length=410, Percent_Identity=98.0487804878049, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI1787065, Length=376, Percent_Identity=41.4893617021277, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1790146, Length=225, Percent_Identity=28.4444444444444, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994889, Length=223, Percent_Identity=27.3542600896861, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788509, Length=284, Percent_Identity=20.7746478873239, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082312, Length=192, Percent_Identity=26.5625, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44644; Mature: 44513

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHHHH
GMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIAT
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHH
VGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALMHFVHWKVLFAIIAVMGFIS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPV
HHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
ILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLALLII
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
GNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFTLFSNNLPKGTVSASLNMVILMVMSVS
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLAGLLNRVRQHQAAELAEER
HHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHHHH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HHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]