Click here to switch to the map view.

The map label for this gene is melA [H]

Identifier: 82546454

GI number: 82546454

Start: 4191400

End: 4192755

Strand: Direct

Name: melA [H]

Synonym: SBO_4146

Alternate gene names: 82546454

Gene position: 4191400-4192755 (Clockwise)

Preceding gene: 82546446

Following gene: 82546456

Centisome position: 92.73

GC content: 52.21

Gene sequence:

>1356_bases
ATGATGACTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTCGTTAAAAATATTCTTGGTGATGTGTTCCA
TCGAGAGGCGCTGAAAACGGCGCATATTGCCCTGATGGACATTGATCCCACCCGCCTGGAAGAGTCGCATATTGTGGTGC
GTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTACAGGATGCCGAT
TTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCT
GGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTT
GCGAGGACATGACGGAAGTCTGCCCCGATGCCACATTGCTCAACTACGTTAACCCAATGGCGATGAATACCTGGGCGATG
TATGCCCGCTATCCGCATATCAAACAGGTAGGGCTGTGCCATTCGGTGCAGGGAACGGCGGAAGAGCTGGCGCGTGACCT
CAATATCGATCCGTCCACGCTGCGTTACCGTTGCGCTGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGTAAAA
CCGCCGACGGCAGTTACGTGAATCTCTACCCGGAACTGCTGGCGGCTTATGAAGCAGGGCAGGCACCGAAGCCACATATT
CATGGTAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTACTTCGTCACGGAATCGTCAGA
ACATTTTGCCGAATATACGCCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATG
AGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAACGCCTCCCGGATTGAT
ATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGTCGAGTGTGATTTACGGCAACGT
CCGTAACGATGGTTTGATTGATAACCTGCCACAAGGATGTTGCGTGGAAGTAGCCTGTCTGGTTGATGCTAATGGCATTC
AGCCGACCAAAGTCGGTACGCTACCTTCGCATCTGGCCGCCCTGATGCAAACCAACATCAACGTACAGACGCTGCTGACC
GAAGCTATTCTTACGGAAAATCGCGACCGTGTTTACCACGCCGCGATGATGGACCCGCATACTGCCGCCGTGCTGGGCAT
TGACGAAATATATGCTCTTGTTGACGACCTGATTGCCGCCCACGGCGACTGGCTGCCAGGCTGGTTGCACCGTTAA

Upstream 100 bases:

>100_bases
GCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTC
AAGCAAGCCAGGAGATCTGC

Downstream 100 bases:

>100_bases
AACGCGACTAAACGCTACTGCGCCGGGGGATTTATTCCGGCGCACACCTCTGACGATACCAATAACAGAAGGCGGGCGTT
GGTAACAGCGACCCGATACC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPHI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR

Sequences:

>Translated_451_residues
MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPHI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
>Mature_451_residues
MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSAGASGKITCHTQQKEALQDAD
FVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPHI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRID
IKPSREYASTIMNAIWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLT
EAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLHR

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=451, Percent_Identity=98.4478935698448, Blast_Score=936, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=463, Percent_Identity=25.7019438444924, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50668; Mature: 50668

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPHI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGESSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHC
GASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
CCCCEEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCCHHH
MRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHCCHHCCCCHH
EELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPHI
HHHHHHCCCCHHHHEEEECCCCEEEEEEEEECCCCCCCEEEHHHHHHHHHHCCCCCCCCC
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
CCCHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHHHHHHCCCHHHHHHHHH
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGESSVIYGNVRNDGLIDNLPQGC
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCH
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
HEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]