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The map label for this gene is nrfE [H]

Identifier: 82546418

GI number: 82546418

Start: 4147780

End: 4149438

Strand: Direct

Name: nrfE [H]

Synonym: SBO_4105

Alternate gene names: 82546418

Gene position: 4147780-4149438 (Clockwise)

Preceding gene: 82546417

Following gene: 82546419

Centisome position: 91.77

GC content: 57.26

Gene sequence:

>1659_bases
GTGTTGACCCCGTTGACGGCCTTTGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCT
GGCGCAGTGCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTTCTCGGTCATTTATG
TCGCCCAACATAGCTACAGCCTGCTGTCGTGGGAACTCAAACTGGCGGCGGTGTGGGGCGGTCATGAAGGTTCGCTGCTG
CTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGCGCTGTTTGCCTGGCATTATCGGCAGCAAACCGATCCGCTATTTCCGCT
GACGCTAGCCGTTTTATCTCTCATGCTCGCCGCACTGCTACTGTTTGTGGTGCTGTGGTCCGATCCCTTCGTGCGGATAT
TTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATCTTTCATCCACCGCTGCTTTAC
CTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAGTCTACTGCGCGGCGAGTTTGATGCGGCCAGCGC
CCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTACT
GCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAATGCCTCTTTATTACCCTGGCTTTCTGCCACTGCG
CTGCTGCACAGTTTATCCCTGACACGCCAGCGGGGGATTTTTCGCCACTGGTCGCTGTTACTGGCGATAGTTACTCTGAT
GCTGTCGCTGCTGGGCACCTTAATTGTCCGTTCTGGCATTCTGGTTTCGGTTCATGCGTTCGCGCTGGATAACGTCCGCG
CCGTGCCGTTGTTCAGCCTGTTTGCACTGATTAGCCTTGCGTCTCTGGCTCTGTATGGCTGGCGAGCGCGGGACGGTGGC
CCGGCGGTGCGTTTTTCGGGGTTATCGCGGGAAATGTTAATCCTCGCTACGCTGTTGCTGTTTTGCGCAGTGCTACTGAT
CGTGCTGGTGGGAACGCTTTATCCGATGATTTACGGTCTGCTGGGCTGGGGACGCCTCTCCGTTGGCGCGCCGTATTTTA
ACCGCGCGACGTTACCGTTTGGTCTGTTGATGCTGGTGGTGATTGTGCTGGCGACGTTTGTCTCTGGCAAACGCGTGCAG
CTTCCGGCGCTGGTAGCTCATGCGGGCGTGCTGTTATTTGCCGCTGGGATCGTGGTTTCCAGCGTCAGCCGTCAGGAGAT
CAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGCGCCTCGATCTGCAAGCCA
AAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGATGCCGGAGCGG
CGTTTTTACGAAGCACGTCGTCAGCAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGTGGTCAT
GGGTGAAAAAACCGGAGCGGATCGTTACGCTTTTCGCTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGAT
TGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAGGAAGCGCGATGAATAA

Upstream 100 bases:

>100_bases
GGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGT
TGTTAAGTCTCGGGGTCAAC

Downstream 100 bases:

>100_bases
AGGACTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGCGCTCAGGTCGTAGACACCTGGCAATTCGCCAATCCGC
AACAACAGCAACAGGCGTTA

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDAASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
PAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALVAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
RFYEARRQQMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Sequences:

>Translated_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDAASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
PAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALVAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
RFYEARRQQMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE
>Mature_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDAASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
PAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQ
LPALVAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
RFYEARRQQMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGRKRDE

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=552, Percent_Identity=98.0072463768116, Blast_Score=1061, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=584, Percent_Identity=38.527397260274, Blast_Score=337, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 61449; Mature: 61449

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRG
HHHHHHCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHC
EFDAASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
CCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCHHHHHHH
FALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHHHCCHH
RFYEARRQQMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGA
HHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHEEEEHHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC
>Mature Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRG
HHHHHHCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHC
EFDAASARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATA
CCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCHHHHHHH
FALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHHHCCHH
RFYEARRQQMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGA
HHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHEEEEHHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]