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The map label for this gene is hydG [H]

Identifier: 82546346

GI number: 82546346

Start: 4061723

End: 4062949

Strand: Direct

Name: hydG [H]

Synonym: SBO_4025

Alternate gene names: 82546346

Gene position: 4061723-4062949 (Clockwise)

Preceding gene: 82546345

Following gene: 82546350

Centisome position: 89.86

GC content: 56.64

Gene sequence:

>1227_bases
ATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGG
CTGGGGCTATAACGTCGCGCTGGCGAACAGCGGGCGACAGGCGCTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGC
TTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTG
CTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCTGGATTATCTCATCAAGCC
GCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAGGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGG
TGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA
TCGGAAGCCACAGTGCTGATCCACGGCGATTCCGGCACCGGTAAAGAGCTGGTCGCCAGGGCGATTCACGCCAGTAGCGC
ACGTAGCGAAAAACCGCTGGTAACGCTCAACTGTGCGGCACTCAACGAATCCTTGCTGGAATCTGAATTGTTCGGTCACG
AAAAAGGGGCGTTTACTGGAGCCGATAAACGCCGGGAAGGGCGCTTTGTTGAGGCGGACGGCGGCACGCTGTTTCTCGAT
GAAATTGGCGATATCTCGCCGATGATGCAGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAG
CAACCAGACTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTT
TTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT
CTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGA
TCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGCCGG
GGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGGCAAAGTCAGGATATTCAGC
CGCTGGTGGAAGTGGAAAAAGAGGTGA

Upstream 100 bases:

>100_bases
AACAACACGGTGGTACAATTCAGGTCGCAAGCCAGGAGGGAAAAGGCTCAACGTTCACCCTCTGGCTTCCGGTCAATATT
ACGCGTAAGGACCCACAAGG

Downstream 100 bases:

>100_bases
TTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACTGAAGCCGCCCGTCAGTTAGGGATCACGCGTAAAACGTTATTG
GCAAAACTGTCGCGTTAGTT

Product: response regulator of hydrogenase 3 activity

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLPGNIFPNASCRWRLPVRRSRWGKVRIFS
RWWKWKKR

Sequences:

>Translated_408_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLPGNIFPNASCRWRLPVRRSRWGKVRIFS
RWWKWKKR
>Mature_407_residues
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVL
IMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPS
EATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDE
IGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPL
LAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLPGNIFPNASCRWRLPVRRSRWGKVRIFSR
WWKWKKR

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=374, Percent_Identity=99.4652406417112, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=376, Percent_Identity=51.5957446808511, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788905, Length=381, Percent_Identity=46.7191601049869, Blast_Score=344, Evalue=5e-96,
Organism=Escherichia coli, GI1790299, Length=383, Percent_Identity=45.1697127937337, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI87082117, Length=252, Percent_Identity=55.5555555555556, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1789087, Length=242, Percent_Identity=52.8925619834711, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI87082152, Length=241, Percent_Identity=54.7717842323651, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1789233, Length=234, Percent_Identity=48.7179487179487, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=50.4347826086956, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=261, Percent_Identity=45.2107279693487, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1787583, Length=234, Percent_Identity=42.7350427350427, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789828, Length=242, Percent_Identity=38.8429752066116, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081858, Length=208, Percent_Identity=35.0961538461538, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789149, Length=112, Percent_Identity=33.9285714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789402, Length=119, Percent_Identity=32.7731092436975, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=142, Percent_Identity=27.4647887323944, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693157, Length=115, Percent_Identity=34.7826086956522, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=34.4262295081967, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1786784, Length=124, Percent_Identity=27.4193548387097, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1786911, Length=131, Percent_Identity=30.5343511450382, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 45540; Mature: 45409

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMA
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
LAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLPGNIFPNASCRWRLPVRRSRWGKVRIFSRWWKWKKR
HHHHHHHEEEEECCCCCCCCCEEEECCCCCCCCCEEEEEHHHHHCCCC
>Mature Secondary Structure 
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
LAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLPGNIFPNASCRWRLPVRRSRWGKVRIFSRWWKWKKR
HHHHHHHEEEEECCCCCCCCCEEEECCCCCCCCCEEEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]