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The map label for this gene is frwB [H]

Identifier: 82546301

GI number: 82546301

Start: 4002456

End: 4002776

Strand: Direct

Name: frwB [H]

Synonym: SBO_3969

Alternate gene names: 82546301

Gene position: 4002456-4002776 (Clockwise)

Preceding gene: 82546300

Following gene: 82546302

Centisome position: 88.55

GC content: 49.53

Gene sequence:

>321_bases
ATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCGGGAAAGTGC
CGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATG
TGGCGAGCGCTGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTG
CGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTA
A

Upstream 100 bases:

>100_bases
ACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATT
AATTAATTGAGGAACGAGCC

Downstream 100 bases:

>100_bases
GTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAGTTCCTCATGACGAATCGTATCTCTCGCCTCAAAACT
GCACTGTTTGCCAATACCCG

Product: PTS system fructose-like transporter subunit EIIB

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MTKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIV
RVNISDAVKRADAIMSKIEAHLAQTA

Sequences:

>Translated_106_residues
MTKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIV
RVNISDAVKRADAIMSKIEAHLAQTA
>Mature_105_residues
TKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIVR
VNISDAVKRADAIMSKIEAHLAQTA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=106, Percent_Identity=99.0566037735849, Blast_Score=209, Evalue=4e-56,
Organism=Escherichia coli, GI1788492, Length=88, Percent_Identity=53.4090909090909, Blast_Score=98, Evalue=9e-23,
Organism=Escherichia coli, GI87082348, Length=98, Percent_Identity=48.9795918367347, Blast_Score=97, Evalue=2e-22,
Organism=Escherichia coli, GI1788730, Length=101, Percent_Identity=44.5544554455446, Blast_Score=95, Evalue=1e-21,
Organism=Escherichia coli, GI1790390, Length=98, Percent_Identity=42.8571428571429, Blast_Score=83, Evalue=3e-18,
Organism=Escherichia coli, GI1786951, Length=94, Percent_Identity=42.5531914893617, Blast_Score=80, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 11291; Mature: 11160

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIGLENELTAEDVASADMV
CCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHCCCCEE
ILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHLAQTA
EEEECCCCEEHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIGLENELTAEDVASADMV
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHCCCCEE
ILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHLAQTA
EEEECCCCEEHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]