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The map label for this gene is insB [H]

Identifier: 82546288

GI number: 82546288

Start: 3985181

End: 3985495

Strand: Reverse

Name: insB [H]

Synonym: SBO_3956

Alternate gene names: 82546288

Gene position: 3985495-3985181 (Counterclockwise)

Preceding gene: 82546289

Following gene: 82546284

Centisome position: 88.18

GC content: 42.54

Gene sequence:

>315_bases
ATGTTGACCACTGACGAATGGGGTAGTTACACCAGAGAGTTACCGAAGGAAAAGCATCTGACTGGTAAGATTTTTACTCA
GCGCATTGAGCGCAACAATCTGACCCTGAGAACCCGAATCAAACGTCTGGCCCGCAGAACCATCTGTTTCTCGCGCTCTG
TAGAACTTCATGAAAAAGTCATCGGTGCTTTTATCGAAAATATATGTTCTACTAATTGGAGTCATCACCCTTCTGATGAG
CTTTCCTTGGATGATTACCAGACTGAATACACAGCCAAAGATTTTTACCTCATTCTGCTCTTTTTTCTCAAATGA

Upstream 100 bases:

>100_bases
ACAACACGAAAACTGGTGGTGTTCTGGCCTACACTTTTGGCCCCCGGACTGACGAAACCTGTCGTGAATTGCTGGCGCTG
CTTACCCTTTTACCATCGGC

Downstream 100 bases:

>100_bases
CATATATTATATTTTACATCATATTCATAGGCATATGACATTCCCCCGCCCCCATTATGTCATTGTGGGTTGATGTTGGG
CTTTCATAAAAAAAACATCT

Product: iso-IS1 ORF2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 104; Mature: 104

Protein sequence:

>104_residues
MLTTDEWGSYTRELPKEKHLTGKIFTQRIERNNLTLRTRIKRLARRTICFSRSVELHEKVIGAFIENICSTNWSHHPSDE
LSLDDYQTEYTAKDFYLILLFFLK

Sequences:

>Translated_104_residues
MLTTDEWGSYTRELPKEKHLTGKIFTQRIERNNLTLRTRIKRLARRTICFSRSVELHEKVIGAFIENICSTNWSHHPSDE
LSLDDYQTEYTAKDFYLILLFFLK
>Mature_104_residues
MLTTDEWGSYTRELPKEKHLTGKIFTQRIERNNLTLRTRIKRLARRTICFSRSVELHEKVIGAFIENICSTNWSHHPSDE
LSLDDYQTEYTAKDFYLILLFFLK

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=62, Percent_Identity=50, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1788203, Length=62, Percent_Identity=50, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1786203, Length=62, Percent_Identity=50, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787223, Length=62, Percent_Identity=50, Blast_Score=66, Evalue=5e-13,
Organism=Escherichia coli, GI1786470, Length=62, Percent_Identity=48.3870967741936, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1786459, Length=62, Percent_Identity=48.3870967741936, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 12440; Mature: 12440

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTDEWGSYTRELPKEKHLTGKIFTQRIERNNLTLRTRIKRLARRTICFSRSVELHEKV
CCCCCCCHHHHHHCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
IGAFIENICSTNWSHHPSDELSLDDYQTEYTAKDFYLILLFFLK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLTTDEWGSYTRELPKEKHLTGKIFTQRIERNNLTLRTRIKRLARRTICFSRSVELHEKV
CCCCCCCHHHHHHCCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
IGAFIENICSTNWSHHPSDELSLDDYQTEYTAKDFYLILLFFLK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]