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The map label for this gene is insG [H]

Identifier: 82546093

GI number: 82546093

Start: 3757254

End: 3758600

Strand: Direct

Name: insG [H]

Synonym: SBO_3740

Alternate gene names: 82546093

Gene position: 3757254-3758600 (Clockwise)

Preceding gene: 82546088

Following gene: 228861769

Centisome position: 83.13

GC content: 53.82

Gene sequence:

>1347_bases
TTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGAC
TTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCC
GTCTTCCCCTCGAAATGATGGTCTGGTGTATTGTTGGCATGGCGCTTGAGCGTAAAGAACCTCTTCACCAGATTATGAAT
CGCCTGGACATCATGCTGCCGGGCAATCGCCCCTTCGTTGCCCCCAGTGCCGTTATTCAGGCCCGCCAGCGCCTGGGAAG
TGAGGCTGTCCGCCGCGTGTTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCATCCGCACTGGTGCGGCCTGA
CCCTGCTGGCCATCGATGGTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAGCCTTCCCCCGCCAGACACAT
GCCGGGAACCCGGCGCTCTACCCGCAGGTCAAAATGGTCTGCCAGATGGAACTGACCAGCCATCTGCTGACGGCTGCAGC
CTTCGGCACGATGAAGAACAGCGAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCGGCGATAACACTCTGACGTTAA
TGGATAAAGGTTATTACTCACTGGGACTGTTAAATGCCCGGAGCCTGGCGGGAGAACACCGCCACTGGATGATACCTCTC
AGAAAGGGAGCGCAATATGAAGAGCTCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAGCTGAAAACCAGCCCGCAGGC
ACGAAAAAAGTGGCCGGGACTGGGAAATGAAGTGACAGCCCGCCTGCTGACCGTGACGCGCAAAGGAAAAGTCTGCCATC
TGCTGACGTCGATGACGGACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACAGTCATCGCTGGGAAATTGAA
CTGGGATACAGGGAGATAAAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAAAAGCCGGAGCTTGTGGAGCA
AGAGCTGTGGGGTGTCTTACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGCGGAACATCTGAAAGGTTACT
GGCCGAATCAACTGAGTTTCTCAGAATCATGCGGAATGGTGATGAGAATGCTGATGACATTGCAGGGCGCTTCACCGGGA
CGTATACCGGAGCTGATGCGCGATCTTGCAAGTATGGGACAACTTGTGAAATTACCGACGAGAAGGGAAAGGGCCTTCCC
GAGAGTGGTAAAGGAGAGGCCCTGGAAATACCCCACAGCCCCGAAAAAGAGCCAGTCAGTTGCTTAA

Upstream 100 bases:

>100_bases
GCCCATAGGCATTCAAAAACGCCTCCGTCTGCTCTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGT
AAGAATCCGGAAACGCTCAC

Downstream 100 bases:

>100_bases
CTGACTGGCATTACCCCCCTTTGAGACTATTTTAGCCACCAGCAAGAAACGTCTATACTCGCAATTTACGCAGAACTTTT
GACGAAAGGACGCCACTTCA

Product: IS4 orf

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIMN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPL
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPG
RIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Sequences:

>Translated_448_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIMN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPL
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPG
RIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA
>Mature_448_residues
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIMN
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPL
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPG
RIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=442, Percent_Identity=98.868778280543, Blast_Score=903, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 51145; Mature: 51145

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMM
CCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHH
VWCIVGMALERKEPLHQIMNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQL
HHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTS
HHCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPL
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHCCCCCEEEEEC
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTD
CCCCCHHHHHHCCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVR
HHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
RRERAFPRVVKERPWKYPTAPKKSQSVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMM
CCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHH
VWCIVGMALERKEPLHQIMNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQL
HHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTS
HHCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPL
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHCCCCCEEEEEC
RKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTD
CCCCCHHHHHHCCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVR
HHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
RRERAFPRVVKERPWKYPTAPKKSQSVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]