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The map label for this gene is insB [H]

Identifier: 82545854

GI number: 82545854

Start: 3479251

End: 3479763

Strand: Direct

Name: insB [H]

Synonym: SBO_3488

Alternate gene names: 82545854

Gene position: 3479251-3479763 (Clockwise)

Preceding gene: 82545850

Following gene: 82545855

Centisome position: 76.98

GC content: 53.41

Gene sequence:

>513_bases
ATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCATTAAAAAACTCAGGCC
GCAGTCGGTAACCTCGCGCATGCAAGCTGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCG
GTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAA
CGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCC
GCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGA
ATCTGAGACAACATCTGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATC
GGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
CGCTATCTCTGCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAA
AATCATTGATATGGCCATGA

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGAT
TATGGGCTTAATTTGTATCG

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MASDVAPVHALWALASTRFYVIKKLRPQSVTSRMQAGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGE
RTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
GHYLNIKHYQ

Sequences:

>Translated_170_residues
MASDVAPVHALWALASTRFYVIKKLRPQSVTSRMQAGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGE
RTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
GHYLNIKHYQ
>Mature_169_residues
ASDVAPVHALWALASTRFYVIKKLRPQSVTSRMQAGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGER
TLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIG
HYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=152, Percent_Identity=90.7894736842105, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1788203, Length=152, Percent_Identity=90.7894736842105, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1786203, Length=152, Percent_Identity=90.7894736842105, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1787223, Length=152, Percent_Identity=90.7894736842105, Blast_Score=290, Evalue=3e-80,
Organism=Escherichia coli, GI1786470, Length=152, Percent_Identity=90.7894736842105, Blast_Score=290, Evalue=5e-80,
Organism=Escherichia coli, GI1786459, Length=152, Percent_Identity=90.7894736842105, Blast_Score=290, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 19855; Mature: 19724

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDVAPVHALWALASTRFYVIKKLRPQSVTSRMQAGSDVIVCAEMDEQWGYVGAKSRQR
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCH
WLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCHHHHH
SKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ASDVAPVHALWALASTRFYVIKKLRPQSVTSRMQAGSDVIVCAEMDEQWGYVGAKSRQR
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCH
WLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCHHHHH
SKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]