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The map label for this gene is insG [H]

Identifier: 82545792

GI number: 82545792

Start: 3412358

End: 3413188

Strand: Reverse

Name: insG [H]

Synonym: SBO_3424

Alternate gene names: 82545792

Gene position: 3413188-3412358 (Counterclockwise)

Preceding gene: 82545793

Following gene: 82545791

Centisome position: 75.52

GC content: 53.67

Gene sequence:

>831_bases
GTGTTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCATCCGCACTGGTGCGGCCTGACCCTGCTGGCCATCGA
TGGTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAGCCTTCCCCCGCCAGACACATGCCGGGAACCCGGCGC
TCTACCCGCAGGTCAAAATGGTCTGCCAGATGGAACTGACCAGCCATCTGCTGACGGCTGCAGCCTTCGGCACGATGAAG
AACAGCGAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCGGCGATAACACTCTGACGTTAATGGATAAAGGTTATTA
CTCACTGGGACTGTTAAATGCCCGGAGCCTGGCGGGAGAACACCGCCACTGGATGATACCTCTCAGAAAGGGAGCGCAAT
ATGAAGAGCTCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAGCTGAAAACCAGCCCGCAGGCACGAAAAAAGTGGCCG
GGACTGGGAAATGAAGTGACAGCCCGCCTGCTGACCGTGACGCGCAAAGGAAAAGTCTGCCATCTGCTGACGTCGATGAC
GGACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACAGTCATCGCTGGGAAATCGAACTGGGATACAGGGAGA
TAAAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAAAAGCCGGAGCTTGTGGAGCAAGAGCTGTGGGGTGTC
TTACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGCGGAACATCTGAAAGGTTACTGGCCGAATCAACTGAG
TTTCTCAGAATCATGCGGAATGGTGATGTGA

Upstream 100 bases:

>100_bases
AATCGCCTGGACATCATGCTGCCGGGCAATCGCCCCCTTCGTTGCCCCCAGTGCCGTTATTCAGGCCCGCCAGCGCCTGG
GAAGTGAGGCTGTCCGCCGC

Downstream 100 bases:

>100_bases
ACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGA
AGTCCGTCAGAGGGCGATTC

Product: IS4 orf

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMK
NSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWP
GLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGV
LLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM

Sequences:

>Translated_276_residues
MFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMK
NSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWP
GLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGV
LLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM
>Mature_276_residues
MFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMK
NSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWP
GLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGV
LLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=276, Percent_Identity=98.1884057971015, Blast_Score=568, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 31511; Mature: 31511

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKM
CCCCHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCHHHHHCCC
HRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVC
CCEEEEECCCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCHH
HLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGV
HHHHHHHHHHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKM
CCCCHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNARSLAGE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCHHHHHCCC
HRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVC
CCEEEEECCCCCCHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHHCCCCHH
HLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGV
HHHHHHHHHHHCCCCCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]