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The map label for this gene is kefB

Identifier: 82545699

GI number: 82545699

Start: 3314752

End: 3316563

Strand: Reverse

Name: kefB

Synonym: SBO_3330

Alternate gene names: 82545699

Gene position: 3316563-3314752 (Counterclockwise)

Preceding gene: 82545700

Following gene: 82545698

Centisome position: 73.38

GC content: 54.8

Gene sequence:

>1812_bases
ATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGTTGGCATCGCGGCT
GGGGATTGGCGCTGTGTTGGGATATTTGCTGGCAGGGATTGCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCG
ATGAGATCCTCCACTTTTCGGAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTT
TGGCAACTGCGGCGTTCGATTTTTGGCGTAGGCGCGGCACAGGTGCTGTTAAGCGCGGCGTTGCTGGCGGGATTATTGAT
GCTGACGGATTTCGCCTGGCAGGCGGCGGTGGTCGGTGGCATTGGCCTTGCGATGTCTTCAACTGCAATGGCGTTGCAAT
TGATGCGTGAGAAAGGGATGAATCGCAGCGAATCCGGCCAGCTCGGGTTTTCGGTTCTGCTGTTTCAGGATCTGGCAGTG
ATCCCAGCACTGGCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGGATGAAGATCGGCATGAAGGT
GCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTACTGCGTCCGGTATTCCGCTTTATTGCAGCTTCTGGCGTGC
GGGAAGTGTTCACCGCCGCGACGCTGCTGCTGGTGTTGGGTTCCGCATTGTTTATGGATGCGCTGGGGCTGTCGATGGCG
CTCGGTACATTTATTGCGGGCGTGCTGCTGGCGGAAAGTGAATATCGCCATGAACTGGAAACGGCTATCGATCCCTTCAA
AGGCTTGCTGCTCGGTTTGTTCTTTATCTCTGTCGGCATGTCGCTCAACCTCGGGGTGCTTTATACCCATCTGTTGTGGG
TAGTGATAAGCGTGGTTGTGCTGGTGGCGGTGAAAATTCTCGTGCTGTATCTGCTGGCGCGATTGTATGGCGTGCGTAGC
TCAGAGCGGATGCAGTTTGCTGGCGTGTTGAGTCAGGGCGGTGAGTTTGCCTTTGTCCTCTTTTCTACCGCTTCTTCACA
ACGTTTATTCCAGGGCGACCAGATGGCGCTGTTGCTGGTGACGGTGACGGTGACGCTTTCCATGATGACCACGCCGTTGC
TGATGAAGCTGGTGGATAAATGGTTATCCCGCCAGTTTAACGGACCGGAAGAAGAAGACGAAAAACCGTGGGTCAACGAT
GATAAACCCCAGGTCATTGTCGTGGGCTTCGGGCGTTTTGGTCAGGTGATTGGTCGTTTGCTGATGGCAAATAAAATGCG
CATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATATGGCTACAAAGTTTATTACGGCGACGCCA
CGCAGGTCGATCTATTACGTTCTGCGGGTGCAGAGGCCGCTGAGTCTATCGTCATTACCTGTAACGAGCCGGAAGACACC
ATGAAGCTGGTGGAAATATGCCAGCAGCACTTTCCGCATTTGCATATTCTTGCGCGAGCGCGCGGACGTGTGGAAGCGCA
TGAGTTATTACAGGCAGGGGTGACGCAGTTTTCCCGTGAAACATTCTCCAGTGCGTTAGAGCTGGGGCGCAAGACTTTGG
TCACGCTTGGGATGCATCCGCATCAGGCGCAGCGGGCGCAACTGCATTTTCGCCGCCTGGATATGCGGATGCTGCGAGAG
CTCATCCCAATGCATGCCGATACTGTACAAATTTCTCGCGCCAGGGAAGCCCGACGCGAACTGGAAGAGATTTTCCAGCG
TGAAATGCAACAAGAACGACGCCAGCTGGACGGCTGGGATGAATTTGAGTAG

Upstream 100 bases:

>100_bases
TTATTTACTGGGCGAGACGGCAAAGCGCAAAGGAGCTGGCGAACCACGCCAGAGCCTACGGTGACTGGCTGGCAAATCCG
CTGTCTCCAGGAGGCCGCTG

Downstream 100 bases:

>100_bases
AGGGTAAAGATGGCAATCCGAAAACGTTTTATTGCGGGCGCAAAATGCCCGGCCTGTCAGGCACAGGATTCAATGGCGAT
GTGGCGCGAAAATAATATTG

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKL
WQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRS
SERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVND
DKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDT
MKLVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRE
LIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE

Sequences:

>Translated_603_residues
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKL
WQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRS
SERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVND
DKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDT
MKLVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRE
LIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE
>Mature_603_residues
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKL
WQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRS
SERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVND
DKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDT
MKLVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRE
LIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain

Homologues:

Organism=Homo sapiens, GI157388921, Length=358, Percent_Identity=26.8156424581006, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI1789749, Length=603, Percent_Identity=99.5024875621891, Blast_Score=1130, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=609, Percent_Identity=43.8423645320197, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1786685, Length=529, Percent_Identity=30.2457466918715, Blast_Score=192, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KEFB_SHIBS (Q31VU0)

Other databases:

- EMBL:   CP000036
- RefSeq:   YP_409646.1
- ProteinModelPortal:   Q31VU0
- SMR:   Q31VU0
- EnsemblBacteria:   EBESCT00000075945
- GeneID:   3782313
- GenomeReviews:   CP000036_GR
- KEGG:   sbo:SBO_3330
- GeneTree:   EBGT00050000011199
- HOGENOM:   HBG428809
- OMA:   HEIIMEN
- PhylomeDB:   Q31VU0
- ProtClustDB:   PRK03659
- BioCyc:   SBOY300268:SBO_3330-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01412
- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00932

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N

EC number: NA

Molecular weight: Translated: 66627; Mature: 66627

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51201 RCK_N

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c3643b8)-; HASH(0x1c57b710)-; HASH(0x1bde8d20)-; HASH(0x1bfd3c14)-; HASH(0x1c4c18d8)-; HASH(0x1c537534)-; HASH(0x1c15d254)-; HASH(0x1c369148)-; HASH(0x1c5e7f30)-; HASH(0x1b3f0624)-; HASH(0x1c5f1198)-; HASH(0x1c4fed0c)-; HASH(0x1c4c1a64)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCH
>Mature Secondary Structure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CCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA