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The map label for this gene is insG [H]

Identifier: 82545687

GI number: 82545687

Start: 3303801

End: 3305087

Strand: Direct

Name: insG [H]

Synonym: SBO_3318

Alternate gene names: 82545687

Gene position: 3303801-3305087 (Clockwise)

Preceding gene: 82545653

Following gene: 82545697

Centisome position: 73.1

GC content: 54.55

Gene sequence:

>1287_bases
TTGTCTGGAGATTCAGGGGGCCAGTCTAGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTAC
TGTAACGCTACGCAAGCGCCGTCTTCCCCTCGAAATGATGGTCTGGTGTATTGTTGGCATGGCGCTTGAGCGTAAAGAAC
CTCTTCACCAGATTGTGAATCGCCTGGACATCATGCTGCCGGGCAATCGCCCCTTCGTTGCCCCCAGTGCCGTTATTCAG
GCCCGCCAGCGCCTGGGAAGTGAGGCTGTCCGCCGCGTGTTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCA
TCCGCACTGGTGCGGCCTGACCCTGCTGGCCATCGATGGTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAG
CCTTCCCCCGCCAGACACATGCCGGGAACCCGGCGCTCCACCCGCAGGTCAAAATGGTCTGCCAGATGGAACTGACCAGC
CATCTGCTGACGGCTGCAGCCTTCGGCACGATGAAGAACAGCGAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCGG
CGATAACACTCTGACGTTAATGGATAAAGGTTATTACTCACTGGGACTGTTAAATGCCTGGAGCCTGGCGGGAGAACACC
GCCACTGGATGATACCTCTCAGAAAGGGAGCGCAATATGAAGAGCTCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAG
CTGAAAACCAGCCCGCAGGCACGAAAAAAGTGGCCGGGACTGGGAAATGAAGTGACAGCCCGCCTGCTGACCGTGACGCG
CAAAGGAAAAGTCTGCCATCTGCTGACGTCGATGACGGACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACA
GTCATCGCTGGGAAATTGAACTGGGATACAGGGAGATAAAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAA
AAGCCGGAGCTTGTGGAGCAAGAGCTGTGGGGTGTCTTACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGC
GGAACATCTGAAAGGTTACTGGCCGAATCAACTGAGTTTCTCAGAATCATGCGGAATGGTGATGAGAATGCTGATGACAT
TGCAGGGCGCTTCACCGGGACGTATACCGGAGCTGATGCGCGATCTTGCAAGTATGGGACAACTTGTGAAATTACCGACG
AGAAGGGGAAGGGCCTTCCCGAGAGTGGTAAAGGAGAGGCCCTGGAAATACCCCACAGCCCCGAAAAAGAGCCAGTCAGT
TGCTTAA

Upstream 100 bases:

>100_bases
GCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTACTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATC
ACTTATTTAAGTGATACTGG

Downstream 100 bases:

>100_bases
CTGACTGGCATTACTGCCTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCC
GTTAATCTTTAACTTTAATT

Product: IS4 orf

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSGDSGGQSRDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQ
ARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALHPQVKMVCQMELTS
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVK
LKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
RRGRAFPRVVKERPWKYPTAPKKSQSVA

Sequences:

>Translated_428_residues
MSGDSGGQSRDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQ
ARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALHPQVKMVCQMELTS
HLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVK
LKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
RRGRAFPRVVKERPWKYPTAPKKSQSVA
>Mature_427_residues
SGDSGGQSRDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQA
RQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALHPQVKMVCQMELTSH
LLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKL
KTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKK
PELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTR
RGRAFPRVVKERPWKYPTAPKKSQSVA

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=418, Percent_Identity=98.8038277511962, Blast_Score=855, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 48583; Mature: 48452

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDSGGQSRDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHHH
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDG
HHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
VFWRTPDTPENDAAFPRQTHAGNPALHPQVKMVCQMELTSHLLTAAAFGTMKNSENELAE
EEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVK
HHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCEEE
LKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
ECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCEEEE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSF
ECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
PKKSQSVA
CCCCCCCC
>Mature Secondary Structure 
SGDSGGQSRDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVN
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHHH
RLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDG
HHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
VFWRTPDTPENDAAFPRQTHAGNPALHPQVKMVCQMELTSHLLTAAAFGTMKNSENELAE
EEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVK
HHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCEEE
LKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIE
ECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCEEEE
LGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSF
ECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCC
PKKSQSVA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]