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The map label for this gene is gspK [H]

Identifier: 82545408

GI number: 82545408

Start: 3018827

End: 3019738

Strand: Direct

Name: gspK [H]

Synonym: SBO_3019

Alternate gene names: 82545408

Gene position: 3018827-3019738 (Clockwise)

Preceding gene: 82545407

Following gene: 82545409

Centisome position: 66.79

GC content: 56.03

Gene sequence:

>912_bases
ATGCTGGTAACCATCACGCTTTCCGGGCGAATGCAGCAACAACTTGGGCGAACGCGCAGCCAGCAGGAGTACCAGCAGGC
GCTGTGGTACAGCGCCAGTGCGGAAAGCCTGGCGCTGAGCGCGCTCAGTCTGAGCCTGAAAAATGAAAAGCGCGTGCATC
TGGCACAGCCGTGGGCATCCGGCCCTCGTTTCTTCCCTCTGCCGCAGGGGCAAATTGCGGTCACCCTGCGTGACGCACAG
GCTTGCTTTAACCTGAATGCCCTTGCTCAGCCGACAACGGCGTCGCGTCCGCTTGCGGTACAACAGTTGATTGCCCTGAT
CTCGCGCCTGGATGTGCCTGCTTATCGGGCCGAACTGATAGCCGAAAGCCTGTGGGAGTTTATTGACGAGGACCGCAGCG
TGCAGACACGTCTGGGCCGTGAAGACAGCGAGTATCTCGCCCGTTCGGTGCCGTTCTACGCCGCCAATCAACCGCTGGCT
GATATCAGTGAGATGCGCGTGGTACAGGGAATGGACGCCGGGCTTTATCAAAAACTGAAACCGCTGGTCTGTGCGCTGCC
GATGATCCGCCAGCAAATCAACATCAATACTTTAGACGTCACGCAAAGTGTGATTCTTGAGGCGCTGTTTGACCCGTGGT
TAAGCCCCGTTCAGGCGCGGGCGTTATTACAACAACGTCCGGCGAAGGGCTGGGAAGATGTCGATCAGTTTCTTGCACAG
CCGCTACTTGCTGACGTCGATGAGCGTACTAAAAAACAGCTAAAAACCGTCCTGAGCGTGGACAGCAATTACTTCTGGCT
GCGTTCAGATATCACCGTGAATGAGATTGAACTGACGATGAACTCGTTAATTGTCCGCATGGGCCCACAACACTTTTCGG
TTCTCTGGCATCAGACAGGAGAAAGTGAGTGA

Upstream 100 bases:

>100_bases
AACGCATCTGGTTGTTACGCGGGCCGCAATAATCATGATCACCTCACCACCAAAACGCGGAATGGCACTGGTCGTGGTGC
TGGTATTGCTGGCGGTTATG

Downstream 100 bases:

>100_bases
GTTCCATCCTTGAGATTTTTTTCCCGCTTTGCGCCGCTGATCCCATCCATTGGCAGCGCCGTACACCCGATGTGGAGCAC
GGTATCTGGTCTGACGTTGC

Product: type II secretion protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQPLA
DISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININTLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQ
PLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE

Sequences:

>Translated_303_residues
MLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQPLA
DISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININTLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQ
PLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE
>Mature_303_residues
MLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQPLA
DISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININTLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQ
PLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=285, Percent_Identity=34.7368421052632, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 34220; Mature: 34220

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWAS
CEEEEEECCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
GPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELI
CCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
AESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLK
HHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
PLVCALPMIRQQININTLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
PLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHEECCC
ESE
CCC
>Mature Secondary Structure
MLVTITLSGRMQQQLGRTRSQQEYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWAS
CEEEEEECCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCC
GPRFFPLPQGQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELI
CCEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
AESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLK
HHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
PLVCALPMIRQQININTLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
PLLADVDERTKKQLKTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTG
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHEECCC
ESE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]