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The map label for this gene is gspE [H]

Identifier: 82545402

GI number: 82545402

Start: 3014040

End: 3015392

Strand: Direct

Name: gspE [H]

Synonym: SBO_3013

Alternate gene names: 82545402

Gene position: 3014040-3015392 (Clockwise)

Preceding gene: 82545401

Following gene: 82545403

Centisome position: 66.68

GC content: 59.2

Gene sequence:

>1353_bases
ATGGTGCCTGTAGCACAGGAAACCACCGCCAAGACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCGGAAGAACTGCCAGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCATCGCTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGTCCCGACTGCCGTCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGCATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTCCGCGGACGGACTGCCATCCATGAGCTGACGTGGTGGATGCCACATTGCGTGATTTGA

Upstream 100 bases:

>100_bases
TAAGCCTGATGCCGCACACCGCACAGCCGGTACTGCCAGCGCAAAACCAGGCCTTACCGCCGGAAGTTCGCGCGTTCCTC
AATGCCGGGAGAACGCGTTA

Downstream 100 bases:

>100_bases
TCCACCGTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAG
AAAGTGCTCGCCGGAGAAAC

Product: type II secretion protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MVPVAQETTAKTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELTWWMPHCVI

Sequences:

>Translated_450_residues
MVPVAQETTAKTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELTWWMPHCVI
>Mature_450_residues
MVPVAQETTAKTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELTWWMPHCVI

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=421, Percent_Identity=59.8574821852732, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1786296, Length=329, Percent_Identity=47.4164133738602, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI87082188, Length=253, Percent_Identity=39.9209486166008, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 50135; Mature: 50135

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVAQETTAKTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLR
CCCCCCCCCCCEEECCCHHHCHHHEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEEEH
QIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKL
HCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
INAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLRPGRKLASLLVSRIKVMARLD
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHH
IAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
HHHHCCCCCCCEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCH
VNTRVGMTFARGLRAILRQDPDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGA
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEHHCCHHHHH
ITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPATDEEKRLLGITDARTVTLYH
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCCEEEEEEC
PQGCPACNHKGFRGRTAIHELTWWMPHCVI
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVPVAQETTAKTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLR
CCCCCCCCCCCEEECCCHHHCHHHEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEEEH
QIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKL
HCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
INAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLRPGRKLASLLVSRIKVMARLD
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHH
IAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
HHHHCCCCCCCEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCH
VNTRVGMTFARGLRAILRQDPDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGA
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEEHHCCHHHHH
ITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPATDEEKRLLGITDARTVTLYH
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCCEEEEEEC
PQGCPACNHKGFRGRTAIHELTWWMPHCVI
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]