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The map label for this gene is cca [H]

Identifier: 82545310

GI number: 82545310

Start: 2916537

End: 2917775

Strand: Direct

Name: cca [H]

Synonym: SBO_2912

Alternate gene names: 82545310

Gene position: 2916537-2917775 (Clockwise)

Preceding gene: 82545309

Following gene: 82545313

Centisome position: 64.53

GC content: 56.17

Gene sequence:

>1239_bases
GTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGT
CGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAA
CGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCG
GATGTCACGCTGGAAGATGATCTTAAGCGTCGCGATCTGACCATTAATGCGCTGGCCCAGGACGATAACGGCGAGATTAT
CGACCCGTACAACGGTCTGGGCGATCTGCAAAATCGTCTGTTGCGCCATGTTTCCCCCGCTTTTGGCGAAGATCCGTTAC
GCGTTCTGCGCGTGGCGCGTTTTGCTGCGCGTTATGCCCACCTCGGTTTTCGTATTGCCGATGAAACTCTGACGCTGATG
CGCGAGATGACCCATGCGGGTGAACTGGAACACCTGACGCCTGAACGGGTATGGAAAGAGACGGAAAGCGCCCTCACCAC
CCGCAATCCCCAGGTGTTCTTCCAGGTACTGCGCGATTGCGGTGCACTTCGCGTTTTATTCCCGGAAATTGACGCCCTGT
TTGGCGTTCCGGCCCCTGCCAGGTGGCATCCGGAAATCGACACCGGTATTCATACCTTAATGACGCTCTCAATGGCGGCG
ATGCTGAGTCCGCAGGTTGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGACTGACGCCGCCAGAACTTTG
GCCGCGTCATCATGGGCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAA
TTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTC
AAATTATTTGATTCCATCGATGCCTGGCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTACG
CGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGTTGGTTGCGCGAAGCCTGGGAAGTGGCGCAGTCAGTGC
CGACAAAAGCGGTCGTTGAAGCGGGATTTAAAGGTGTGGAGATTCGCGAGGAGCTGACCCGACGGCGGATTGCGGCGGTA
GCCAGTTGGAAGGAACAACGTTGCCCAAAGCCTGACTGA

Upstream 100 bases:

>100_bases
CCCAACGCGCAAACGCAAAGAGCGCTGGATGAACTAAATCGCCTTCTTCGTTGCACTGTCTTATTATTAGGAATTGTGTA
TTTGACGAAGGGATGATGGC

Downstream 100 bases:

>100_bases
GTATAAAACTAAACGTTAACACGAACCATCCCCTCGCCCCTTCGGGGAGAGGGTGAGGGGAACAGACCAGCACCGGTGCG
AACAATCACCCCCACCCCGG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEDDLKRRDLTINALAQDDNGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIV
KLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREELTRRRIAAV
ASWKEQRCPKPD

Sequences:

>Translated_412_residues
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEDDLKRRDLTINALAQDDNGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIV
KLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREELTRRRIAAV
ASWKEQRCPKPD
>Mature_412_residues
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAP
DVTLEDDLKRRDLTINALAQDDNGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIV
KLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREELTRRRIAAV
ASWKEQRCPKPD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=412, Percent_Identity=99.2718446601942, Blast_Score=841, Evalue=0.0,
Organism=Escherichia coli, GI87081691, Length=323, Percent_Identity=28.1733746130031, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46512; Mature: 46512

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDDNGEIIDPYNGLGDLQNRL
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLMREMTHAGELEHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
TESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
HHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLARL
HHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESADYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RWLREAWEVAQSVPTKAVVEAGFKGVEIREELTRRRIAAVASWKEQRCPKPD
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGRDFPVFLHPQTHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDDNGEIIDPYNGLGDLQNRL
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLMREMTHAGELEHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
TESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPAPARWHPEIDTGIHTLMTLSMAA
HHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPAGVKLVEQLCQRLRVPNEIRDLARL
HHCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQRVEQLALTSEADVRGRTGFESADYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RWLREAWEVAQSVPTKAVVEAGFKGVEIREELTRRRIAAVASWKEQRCPKPD
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA