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The map label for this gene is yffG [H]

Identifier: 82544916

GI number: 82544916

Start: 2473596

End: 2475575

Strand: Reverse

Name: yffG [H]

Synonym: SBO_2484

Alternate gene names: 82544916

Gene position: 2475575-2473596 (Counterclockwise)

Preceding gene: 161984890

Following gene: 82544915

Centisome position: 54.77

GC content: 55.81

Gene sequence:

>1980_bases
ATGAATCGTTTTATTATGGCCAACAGTCAGCAATGTCTGGGTTGTCATGCTTGTGAAATCGCCTGTGTCATGGCTCACAA
TGATGAGCAACATGTCCTGAGCCAACACCATTTTCATCCCCGAATTACGGTTATCAAACATCAGCAGCAACGTAGTGCAG
TGACCTGTCACCATTGTGAAGATGCGCCCTGCGCCCGTAGCTGCCCTAATGGCGCAATCAGCCACGTTGATGACAGCATT
CAGGTCAATCAGCAAAAGTGTATTGGCTGTAAATCCTGCGTGGTGGCCTGTCCGTTTGGTACGATGCAAATCGTCCTGAC
ACCCGTCGCGGCAGGAAAAGTAAAAGCCACGGCGCATAAATGCGACCTTTGTGCAGGGCGCGAAAACGGTCCTGCCTGTG
TCGAGAATTGCCCGGCGGACGCGCTGCAACTGGTCACTGACGTCGCACTCTCCGGCATGGCGAAATCCCGCCGCTTGCGC
ACCGCACGTCAGGAGCATCAACCGTGGCATGCCAGTACCGCTGCACAAGAAATGCCGGTAATGAGTAAAGTCGAACAAAT
GCAGGCAACGCCCGCGCGTGGCGAGCCGGATAAACTGGCGATTGAAGCGCGCAAAACCGGTTTTGATGAAATTTATCTGC
CATTTCGCGCCGACCAGGCACAACGGGAAGCCTCGCGCTGCCTTAAGTGCGGCGAGCACAGCGTTTGTGAATGGACCTGC
CCGCTGCATAACCATATTCCGCAGTGGATTGAACTGGTGAAAGCCGGAAACATCGACGCCGCCGTCGAGCTTTCTCACCA
GACCAACACCCTGCCGGAAATTACCGGACGCGTTTGTCCGCAAGACCGTTTGTGTGAAGGTGCCTGTACTATTCGCGATG
AGCACGGCGCGGTAACTATCGGCAACATTGAACGCTATATTTCAGATCAGGCGTTGGCGAAAGGTTGGCGTCCTGACTTA
AGTCATGTCACCAAAGTGGACAAGCGGGTGGCGATTATCGGGGCAGGTCCGGCAGGGCTGGCCTGTGCGGATGTTCTGAC
CCGCAATGGCGTGGCGGTGACGGTGTACGATCGCCATCCAGAAATCGGTGGCTTGCTCACTTTCGGCATTCCTTCTTTCA
AACTGGATAAATCCCTGCTGGCACGCCGTCGGGAAATCTTCAGCGCGATGGGGATTCACTTCGAACTCAATTGTGAAGTG
GGTAAAGATGTCTCTTTGGATTCGCTTTTGGAACAATACGACGCGGTCTTCGTTGGCGTAGGCACTTACCGTTCCATGAA
AGCGGGTTTACCCAATGAAGATGCGCCGGGCGTTTATGACGCGCTGCCGTTCCTCATCGCCAACACTAAACAGGTGATGG
GGCTCGAAGAGCTACCGGAAGAGCCGTTTATCAATACCGCCGGACTTAACGTCGTGGTACTGGGCGGCGGCGACACCGCG
ATGGACTGTGTGCGTACTGCGCTGCGCCACGGCGCGAGTAACGTCACCTGCGCTTATCGTCGTGATGAAGCTAACATGCC
AGGCTCGAAGAAAGAGGTGAAGAACGCCCGCGAAGAGGGGGCCAACTTCGAATTTAACGTCCAGCCGGTGGCGCTTGAGC
TGAATGAACAAGGTCACGTTTGCGGGATTCGTTTCCTGCGCACGCGTCTTGGCGAGCCGGATGCCCAGGGGCGTCGGCGT
CCAGTACCGGTGGAAGGCAGTGAATTTGTCATGCCAGCCGACGCGGTGATTATGGCGTTTGGCTTCAATCCGCACGGGAT
GCCGTGGCTGGAGTCGCACGGTGTAACGGTAGACAAATGGGGCCGCATCATCGCGGATGTGGAAAGCCAGTACCGTTACC
AGACCACCAATCCGAAAATCTTCGCCGGTGGCGACGCCGTGCGTGGTGCGGATCTGGTGGTTACCGCAATGGCAGAAGGA
CGTCATGCGGCACAGGGGATTATTGACTGGCTGGGGGTAAAATCAGTCAAATCTCACTGA

Upstream 100 bases:

>100_bases
TTATTCTGGGCACATGAACATTGCCGATTCCCCTGTAAGTCGGGTAATAACAACACTCATATAAAGAATAAGGTTTTTAC
AACCAAAAAAGAAGGTCGTT

Downstream 100 bases:

>100_bases
TAGCCTGCGCAGACAAACCCGACTTCACAGCGTAAGATAATTGTTCATTTCGCGCTGTGGAGTCGGTATGACGCAACAAA
TCACCCTCATTAAAGACAAA

Product: oxidoreductase Fe-S binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITVIKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSI
QVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDL
SHVTKVDKRVAIIGAGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEV
GKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRR
PVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEG
RHAAQGIIDWLGVKSVKSH

Sequences:

>Translated_659_residues
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITVIKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSI
QVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDL
SHVTKVDKRVAIIGAGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEV
GKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRR
PVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEG
RHAAQGIIDWLGVKSVKSH
>Mature_659_residues
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITVIKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSI
QVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDL
SHVTKVDKRVAIIGAGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEV
GKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRR
PVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEG
RHAAQGIIDWLGVKSVKSH

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=493, Percent_Identity=29.2089249492901, Blast_Score=164, Evalue=3e-40,
Organism=Escherichia coli, GI1788811, Length=659, Percent_Identity=99.8482549317147, Blast_Score=1368, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=656, Percent_Identity=54.5731707317073, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI1789606, Length=461, Percent_Identity=66.8112798264642, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=32.0712694877506, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1789067, Length=172, Percent_Identity=48.8372093023256, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=33.4872979214781, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI2367245, Length=147, Percent_Identity=52.3809523809524, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI87082179, Length=166, Percent_Identity=44.578313253012, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1789079, Length=185, Percent_Identity=38.3783783783784, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082114, Length=188, Percent_Identity=37.7659574468085, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787122, Length=158, Percent_Identity=31.6455696202532, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI2367345, Length=161, Percent_Identity=33.5403726708075, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787872, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787749, Length=185, Percent_Identity=25.9459459459459, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI226510944, Length=148, Percent_Identity=33.7837837837838, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=168, Percent_Identity=24.4047619047619, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17570289, Length=480, Percent_Identity=33.5416666666667, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71984108, Length=446, Percent_Identity=32.5112107623318, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=30.4347826086957, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=469, Percent_Identity=33.0490405117271, Blast_Score=223, Evalue=9e-59,
Organism=Drosophila melanogaster, GI28574881, Length=471, Percent_Identity=36.3057324840764, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24665539, Length=471, Percent_Identity=36.3057324840764, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24665547, Length=471, Percent_Identity=36.3057324840764, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24665543, Length=471, Percent_Identity=36.3057324840764, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24640763, Length=440, Percent_Identity=28.8636363636364, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI18858217, Length=440, Percent_Identity=28.8636363636364, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 71859; Mature: 71859

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00028 ZINC_FINGER_C2H2_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITVIKHQQQRSAVTCHHCE
CCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHCCCCCEEEEEEEHHHCCCEEEECCC
DAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHK
CCCCHHCCCCCCHHHCCCCCEECHHHHCCCCHHEEECCCCCEEEEEEECCCCCEEECHHH
CDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLRTARQEHQPWHASTAAQEMPV
HHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHH
MSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTI
CHHHCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCHHHHCCCCCEEEECCCEEEE
GNIERYISDQALAKGWRPDLSHVTKVDKRVAIIGAGPAGLACADVLTRNGVAVTVYDRHP
CCHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCC
EIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEVGKDVSLDSLLEQYDAVFVGV
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCEEEEEC
GTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
CCHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHCCCCCCEECCCCEEEEECCCCHH
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHV
HHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCC
CGIRFLRTRLGEPDAQGRRRPVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKW
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCHHCCCCCHHHH
GRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH
HHHHHHHHHHCEEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITVIKHQQQRSAVTCHHCE
CCCEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHCCCCCEEEEEEEHHHCCCEEEECCC
DAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTMQIVLTPVAAGKVKATAHK
CCCCHHCCCCCCHHHCCCCCEECHHHHCCCCHHEEECCCCCEEEEEEECCCCCEEECHHH
CDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLRTARQEHQPWHASTAAQEMPV
HHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHH
MSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTC
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEC
PLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTI
CHHHCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCHHHHCCCCCEEEECCCEEEE
GNIERYISDQALAKGWRPDLSHVTKVDKRVAIIGAGPAGLACADVLTRNGVAVTVYDRHP
CCHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCC
EIGGLLTFGIPSFKLDKSLLARRREIFSAMGIHFELNCEVGKDVSLDSLLEQYDAVFVGV
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCEEEEEC
GTYRSMKAGLPNEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTA
CCHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHCCCCCCEECCCCEEEEECCCCHH
MDCVRTALRHGASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHV
HHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCC
CGIRFLRTRLGEPDAQGRRRPVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKW
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCHHCCCCCHHHH
GRIIADVESQYRYQTTNPKIFAGGDAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH
HHHHHHHHHHCEEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]