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The map label for this gene is rmf

Identifier: 82544723

GI number: 82544723

Start: 2264439

End: 2264606

Strand: Reverse

Name: rmf

Synonym: SBO_2278

Alternate gene names: 82544723

Gene position: 2264606-2264439 (Counterclockwise)

Preceding gene: 82544724

Following gene: 82544720

Centisome position: 50.1

GC content: 54.17

Gene sequence:

>168_bases
ATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA
AATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAA
TGGCCTGA

Upstream 100 bases:

>100_bases
TTGACGGCAGTTATGATTCGCGGTATTGCTTAACTGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAAC
CAGTATGAGGGAAACGAGGC

Downstream 100 bases:

>100_bases
TTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATGATAATCAGAAGGCAG
ACGTATCCTGGAACAGACCG

Product: ribosome modulation factor

Products: NA

Alternate protein names: Protein E

Number of amino acids: Translated: 55; Mature: 55

Protein sequence:

>55_residues
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGWREAMADRVVMA

Sequences:

>Translated_55_residues
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGWREAMADRVVMA
>Mature_55_residues
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGWREAMADRVVMA

Specific function: Associates with 70S ribosomes and converts them to a dimeric form (100S ribosomes) which appears during the transition from the exponential growth phase to the stationary phase of E.coli cells

COG id: COG3130

COG function: function code J; Ribosome modulation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787186, Length=55, Percent_Identity=100, Blast_Score=114, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMF_ECO57 (P0AFW3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85622
- PIR:   E90758
- RefSeq:   NP_286828.1
- RefSeq:   NP_309064.1
- ProteinModelPortal:   P0AFW3
- SMR:   P0AFW3
- EnsemblBacteria:   EBESCT00000027154
- EnsemblBacteria:   EBESCT00000055156
- GeneID:   913590
- GeneID:   958936
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1303
- KEGG:   ecs:ECs1037
- GeneTree:   EBGT00050000011120
- HOGENOM:   HBG336266
- OMA:   DARSYWL
- ProtClustDB:   PRK14563
- BioCyc:   ECOL83334:ECS1037-MONOMER
- InterPro:   IPR007040
- InterPro:   IPR023200
- ProDom:   PD051650

Pfam domain/function: PF04957 RMF

EC number: NA

Molecular weight: Translated: 6508; Mature: 6508

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
7.3 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
7.3 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGWREAMADRVVMA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGWREAMADRVVMA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796