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The map label for this gene is insF1 [C]

Identifier: 82544538

GI number: 82544538

Start: 2050359

End: 2051210

Strand: Reverse

Name: insF1 [C]

Synonym: SBO_2078

Alternate gene names: 82544538

Gene position: 2051210-2050359 (Counterclockwise)

Preceding gene: 161984917

Following gene: 82544532

Centisome position: 45.38

GC content: 50.47

Gene sequence:

>852_bases
ATGTCTGGAAATATAGGGGCAAATCCATTTGGTGTATCCAGGAGCGGTTATTACAACTGGGTACAGCATGAACCCTCAGA
CAGAAAACAAAGTGATGAGCGGCTAAAACTGGAGATTAAGGTGGCACATATCCGCACTCGCGAAACATATGGAACCCGGC
GGCTCCAGACGGAGCTGGCAGAGAATGGCATCATCGTTGGTCGTGACCGACTGGCACGTCTTCGTAAGGAGCTAAGGCTA
CGCTGTAAGCAGAAACGCAAGTTCAGAGCGACTACGAACCCGAACCACAATCTGCCAGTTGCGCCAAATCTGCTGAACCA
GACGTTCGCTCCTACAGCACCAAATCAGGTCTGGGTGGCGGACCTGACGTATGTTGCCACACAGGAGGGATGGTTGTACC
TCGCTGGCATCAAAGATGTTTATACGTGCGAAATTGTCGGCTACGCCATGGGAGAGCGCATGACAAAAGAGCTGACAGGT
AAAGCCCTGTTTATGGCGCTCAGGAGCCAACGCCCACCTGCCGGGCTAATCCACCACTCTGATCGAGGTTCACAGTACTG
CGCATACGATTACCGGGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTAAAGGTCACTGTTACGACAACG
CTCCGATGGAAAGCTTCTGGGGAACGCTGAAAAATGAGAGCCTGAGCCACTATCGTTTTAATAACCGGGATGAAGCCATC
TCAGTAATACGGGAATACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCTGGGGAATATCTCCCCGGCAGC
CTTCAGGGAAAAATATCATCAGATGACTGCTCAAAAAAAGAACAAATGGTAG

Upstream 100 bases:

>100_bases
TCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGT
AATGGGTTAAGTGATAACAG

Downstream 100 bases:

>100_bases
TGTCCGCTATTGCCAGTACACCTCACACACCCGGCAGAACGGGGAAGTCATATTCCCCGTTCTGTGTGGTGACATGGCTG
AAAACACTTTTCAAAATCCC

Product: IS600 ORF2

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSGNIGANPFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
RCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTG
KALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAI
SVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW

Sequences:

>Translated_283_residues
MSGNIGANPFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
RCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTG
KALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAI
SVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW
>Mature_282_residues
SGNIGANPFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLR
CKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGK
ALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAIS
VIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=216, Percent_Identity=32.8703703703704, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1788405, Length=262, Percent_Identity=40.8396946564886, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787263, Length=262, Percent_Identity=40.8396946564886, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1786753, Length=262, Percent_Identity=40.8396946564886, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1786570, Length=262, Percent_Identity=40.8396946564886, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1786490, Length=262, Percent_Identity=40.8396946564886, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1789981, Length=262, Percent_Identity=32.4427480916031, Blast_Score=130, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32891; Mature: 32760

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNIGANPFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELA
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHH
ENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
HCCEEEEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEE
DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHS
EEEEEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
DRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAI
CCCCCEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHH
SVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGNIGANPFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELA
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHH
ENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
HCCEEEEHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCHHHHCCCCCCCCCEEEEE
DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHS
EEEEEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
DRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTLKNESLSHYRFNNRDEAI
CCCCCEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHH
SVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMTAQKKNKW
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]