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The map label for this gene is puuA [H]

Identifier: 82544246

GI number: 82544246

Start: 1741336

End: 1742832

Strand: Direct

Name: puuA [H]

Synonym: SBO_1765

Alternate gene names: 82544246

Gene position: 1741336-1742832 (Clockwise)

Preceding gene: 161984928

Following gene: 82544247

Centisome position: 38.53

GC content: 52.44

Gene sequence:

>1497_bases
TTGCATACAGATTCGAATGGTGGTCATTATATTTTACGCTTTGATAACGAGCGGAAAACAAACCAAAGGCGAAGAATCAT
GGAAACCAATATCGTTGAAGTAGAGAACTTTGTTCAGCAGCCAGAAGAGAGGCGGGGTAGCGCCTTTACGCAGGAAGTGA
AACGCTACCTGGAGCGCTACCCGAATACGCAATATGTTGATGTTTTACTTACTGATTTGAATGGTTGCTTCCGTGGAAAA
CGTATCCCTGTTTCAAGCCTGAAGAAGTTGGAGAAAGGGTGTTATTTCCCGGCCTCGGTGTTTGCAATGGATATTCTGGG
CAACGTAGTAGAAGAAGCGGGTCTGGGTCAGGAAATGGGCGAGCCGGATCGTACTTGTGTTCCTGTTCTCGGTTCCTTAA
CCCCTTCTGCCGCCGATCCAGAGTTTATCGGTCAGATGCTCCTGACCATGGTCGATGAAGATGGCGCTCCCTTTGACGTT
GAGCCGCGGAACGTTCTTAACCGCCTGTGGCAGCAGCTACGCCAGCGCGGATTGTTCCCGGTTGTAGCGGTAGAGCTGGA
GTTCTATTTACTGGATCGTCAGCGCGACGCTGAAGGGTATCTGCAACCGCCCTGCGCGCCCGGCACCGATAACCGCAATA
CACAAAGCCAGGTTTACTCCGTTGATAACCTCAACCACTTCGCCGACGTGCTCAATGATATTGATGAACTGGCGCAGTTA
CAGTTAATTCCCGCCGACGGTGCGGTCGCCGAAGCCTCGCCGGGTCAGTTTGAAATCAACCTTTACCATACCGATAACGT
GCTGGAAGCCTGCGATGATGCGCTGGCGCTAAAACTCCTGGTGCGTCTGATGGCAGAAAAGCATAAGATGCACTCCACTT
TTATGGCGAAGCCGTATGAAGAGCACGCGGGCAGCGGGATGCATATCCATATCAGTATGCAAAATAATCGTGGCGAGAAT
GTGCTTTCTGACACAGAAGGCGAAGATTCGCCGCTGCTGAAAAAGATGCTCGCCGGGATGATTGACCTGATGCCGTCGTC
GATGGCGTTGCTGGCACCAAACGTGAACTCGTATCGCCGCTTCCAGCCGGGAATGTATGTGCCGACGCAGGCGTCGTGGG
GCCATAACAACCGCACCGTCGCCCTGCGTATTCCGTGCGGCGACCGTCATAATCACCGCGTGGAATATCGCGTGGCGGGT
GCCGATGCCAACCCATATCTGGTGATGGCAGCGATTTTTGCCGGTATTTTGCATGGCCTTGATAACGAGCTGCCGTTGCA
GGAAGAAGTCGAAGGCAACGGGCTGGAACAGGAAGGCTTACCCTTCCCGATTCGCCAGAGCGATGCCCTGGGTGAGTTTA
TCGAGAATGATCACCTGCGCCGCTATTTAGGCGAACGGTTTTGCCATGTGTATCACGCCTGTAAAAATGATGAGCTGTTG
CAGTTTGAGCGCCTCATCACAGAAACAGAAATTGAGTGGATGTTGAAAAACGCATAG

Upstream 100 bases:

>100_bases
ATTACTGCGCTTTAAATGACCTTTATGTTCAATATTTTTTCAATCTAGCAGTGGGTTTTCATTTTTGCAAACTAAATTTA
ACATTTGGCAAACATTTAGT

Downstream 100 bases:

>100_bases
TACCCCTTGCGGGGTTGTTTCATCGACCTGAGCGGAGCCAGGCCTGACATTTGCTGGTAATCCAGACATTTCCCGGATGT
CGCGTTATGGAGCGCAGGCG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MHTDSNGGHYILRFDNERKTNQRRRIMETNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGK
RIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDV
EPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQL
QLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEHAGSGMHIHISMQNNRGEN
VLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAG
ADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELL
QFERLITETEIEWMLKNA

Sequences:

>Translated_498_residues
MHTDSNGGHYILRFDNERKTNQRRRIMETNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGK
RIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDV
EPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQL
QLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEHAGSGMHIHISMQNNRGEN
VLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAG
ADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELL
QFERLITETEIEWMLKNA
>Mature_498_residues
MHTDSNGGHYILRFDNERKTNQRRRIMETNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGK
RIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDV
EPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQL
QLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEHAGSGMHIHISMQNNRGEN
VLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAG
ADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELL
QFERLITETEIEWMLKNA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=440, Percent_Identity=25.4545454545455, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI87081870, Length=472, Percent_Identity=98.9406779661017, Blast_Score=970, Evalue=0.0,
Organism=Escherichia coli, GI1790301, Length=398, Percent_Identity=25.1256281407035, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 56388; Mature: 56388

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTDSNGGHYILRFDNERKTNQRRRIMETNIVEVENFVQQPEERRGSAFTQEVKRYLERY
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHC
PNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMG
CCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
EPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFP
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VVAVELEFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQL
EEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHE
QLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYE
EEECCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EHAGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHH
FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGL
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCC
DNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELL
CCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QFERLITETEIEWMLKNA
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHTDSNGGHYILRFDNERKTNQRRRIMETNIVEVENFVQQPEERRGSAFTQEVKRYLERY
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHC
PNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMG
CCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
EPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFP
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VVAVELEFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQL
EEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHE
QLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYE
EEECCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EHAGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHH
FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGL
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCC
DNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELL
CCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QFERLITETEIEWMLKNA
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]