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The map label for this gene is ydaO [H]

Identifier: 82544200

GI number: 82544200

Start: 1697677

End: 1698612

Strand: Direct

Name: ydaO [H]

Synonym: SBO_1717

Alternate gene names: 82544200

Gene position: 1697677-1698612 (Clockwise)

Preceding gene: 82544199

Following gene: 82544203

Centisome position: 37.56

GC content: 48.82

Gene sequence:

>936_bases
ATGCAAGAAAATCAACAAATTACAAAGAAAGAACAATACAGCCTGAACAAATTACAAAAACGTCTACGTCGTAACGTGGG
CGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCAGGGGGTAAAGACAGCTATA
CCATGCTGGAGATTCTGCGCAATTTGCAGCAAAGCGCGCCAATCAATTTTTCGCTGGTGGCTGTTAACCTCGATCAAAAG
CAACCGGGCTTCCCGGAACACGTTCTGCCCGAGTATCTTGAAAAGCTGGGCGTTGAGTACAAGATTGTTGAAGAGAACAC
TTACGGTATCGTGAAAGAGAAGATTCCAGAGGGCAAAACCACTTGCTCACTGTGTTCTCGGCTACGTCGCGGTATCCTTT
ATCGCACCGCAACGGAACTGGGGACGACGAAGATCGCGTTGGGTCACCATCGTGACGATATCCTGCAAACGTTGTTCTTA
AATATGTTCTACGGCGGCAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTTATTCGTCC
GCTGGCCTACTGCCGCGAGAAAGATATTCAGCGATTTGCCGATGCAAAAGCGTTCCCGATTATTCCGTGCAACCTGTGCG
GTTCACAGCCTAACCTGCAACGTCAGGTGATTGCTGACATGTTGCGTGACTGGGATAAACGTTATCCAGGGCGTATCGAG
ACGATGTTCAGCGCGATGCAGAATGTGGTGCCGTCGCATCTGTGCGATACCAACCTGTTCGATTTCAAAGGCATCACCCA
CGGTTCTGAAGTGGTTAACGGGGGTGATCTGGCGTTTGATCGCGAAGAGATCCCACTACAACCGGCGGGCTGGCAGCCAG
AAGAAGATGAAAATCAGTTGGATGAGTTACGGCTGAATGTGGTTGAAGTGAAATAA

Upstream 100 bases:

>100_bases
TGACATGAACGACGAACAGATAGCCATGTTGAATGCGCGGTTATCGTAGTTGCAAAGTTTGCCCCCAATTTGCCCCATTT
AGTACCAGAGAACTGAAATA

Downstream 100 bases:

>100_bases
CCAGGATAGCGCCCGATGCGCAAGCGTATCGGGCTACTCTTATGGAGGCCGGATAAGACGCGGCCAACGTCGCATCCGGC
AATGCCGAAGAAGATGTTTA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK

Sequences:

>Translated_311_residues
MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK
>Mature_311_residues
MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=311, Percent_Identity=99.0353697749196, Blast_Score=641, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193211090, Length=232, Percent_Identity=31.8965517241379, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17540090, Length=235, Percent_Identity=26.8085106382979, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35518; Mature: 35518

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQL
HHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHH
DELRLNVVEVK
HHHHHEEEECC
>Mature Secondary Structure
MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPAGWQPEEDENQL
HHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCHHHHH
DELRLNVVEVK
HHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA