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The map label for this gene is insB [H]

Identifier: 82544097

GI number: 82544097

Start: 1596453

End: 1596905

Strand: Direct

Name: insB [H]

Synonym: SBO_1606

Alternate gene names: 82544097

Gene position: 1596453-1596905 (Clockwise)

Preceding gene: 161984933

Following gene: 82544098

Centisome position: 35.32

GC content: 52.1

Gene sequence:

>453_bases
ATGTCTGGAAATATAGGGGCAAATCCAGTAACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGA
CGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTG
TGGCGCACGTCTTCGGTGAACGCACTCTGACCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTA
TGGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCG
CATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGG
AGCTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
TCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGT
AATGGGTTAAGTGATAACAG

Downstream 100 bases:

>100_bases
GTTGGCAGCATTACCCAAAGCCGAAGCTGTCAGAACGGTTCTTGAAAATCAACTTTCGATCAGTGAAGGCGCTTCCCGAT
TATCCCTTCCTGAAGGCACT

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MSGNIGANPVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLERLLSLLSAFEVVV
WMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_150_residues
MSGNIGANPVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLERLLSLLSAFEVVV
WMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_149_residues
SGNIGANPVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLERLLSLLSAFEVVVW
MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=141, Percent_Identity=92.9078014184397, Blast_Score=276, Evalue=5e-76,
Organism=Escherichia coli, GI1788203, Length=141, Percent_Identity=92.9078014184397, Blast_Score=276, Evalue=5e-76,
Organism=Escherichia coli, GI1786203, Length=141, Percent_Identity=92.9078014184397, Blast_Score=276, Evalue=5e-76,
Organism=Escherichia coli, GI1787223, Length=141, Percent_Identity=92.9078014184397, Blast_Score=276, Evalue=5e-76,
Organism=Escherichia coli, GI1786470, Length=141, Percent_Identity=92.9078014184397, Blast_Score=275, Evalue=7e-76,
Organism=Escherichia coli, GI1786459, Length=141, Percent_Identity=92.9078014184397, Blast_Score=275, Evalue=7e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 17496; Mature: 17365

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNIGANPVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGE
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RTLTTLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLA
HHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHH
RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGNIGANPVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGE
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RTLTTLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLA
HHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHH
RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]