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The map label for this gene is ynjE [H]

Identifier: 82543840

GI number: 82543840

Start: 1315264

End: 1316586

Strand: Reverse

Name: ynjE [H]

Synonym: SBO_1332

Alternate gene names: 82543840

Gene position: 1316586-1315264 (Counterclockwise)

Preceding gene: 82543841

Following gene: 82543838

Centisome position: 29.13

GC content: 53.59

Gene sequence:

>1323_bases
ATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTG
GGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCG
CGTTTTATAACGGCTGGCCACAAACCTTAAATGGCCCTTCTGGTCATGAACCTGCCGCCTTAAACCTCTCTGCCAGCTGG
CTCGACAAAATGAGCACCGAACAGCTCAACGCGTGGATCAAGCAACATAACCTGAAAGCCGATGCCCCGGTGGCGCTGTA
CGGTAATGACAAAGATGTCGACGCCGTCAAAACGCGACTGCAAAAAGCAGGTTTTACGCATATCTCCATTCTGAGTGACG
CGCTAAGCGAACCTTCCCGTCTACAAAAACTGCCGCACTTTGAGCAGCTGGTTTATCCGCAATGGCTGCACGACCTGCAA
CAAGGTAAAGAGGTTACGGCGAAACCTGCCGGTGACTGGAAAGTCATTGAAGCGGCCTGGGGCGCTCCTAAGCTTTACCT
TATCAGCCATATTCCCGGTGCTGACTACATCGATACCAACGAAGTGGAAAGCGAACCGCTGTGGAACAAAGTTTCTGATG
AACAACTAAAAGCGATGCTGGCAAAACACGGCATTCGCCATGACACCACGGTCATTCTGTACGGGCGTGACGTATACGCT
GGAGCGCGTGTGGCGCAAATTATGCTCTACGCAGGCGTGAAAGATGTGCGTCTGCTGGATGGCGGCTGGCAAACCTGGTC
CGACGCGGGACTGCCTGTTGAGCGAGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCAC
AACCGCAGTTGATGCTCGATATGGAACAGGCCCGTGGACTGCTGCATCGCCAGGATGCATCGCTGGTGAGCATTCGTTCG
TGGCCAGAATTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGAGCACGTTGGGGACA
CGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTA
TGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTT
ATGTACGCACGCGCCATGGGTTGGAAGAATGTTTCCGTGTATGACGGCGGCTGGTACGAATGGAGCAGCGATCCAAAAAA
TCCGGTAGCAACCGGTGAACGCGGCCCGGACAGTAGCAAATAA

Upstream 100 bases:

>100_bases
TGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGA
ATGGCGCACTCAAAACTACA

Downstream 100 bases:

>100_bases
CATTGCAATTACTGACGCTGGAGCGACTTCAGCGTCAGATACCCACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAA
CGCACCGAAGATCCACGCAA

Product: thiosulfate sulfur transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSGFSMKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASW
LDKMSTEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQ
QGKEVTAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYA
GARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRS
WPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETF
MYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK

Sequences:

>Translated_440_residues
MSGFSMKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASW
LDKMSTEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQ
QGKEVTAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYA
GARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRS
WPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETF
MYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK
>Mature_439_residues
SGFSMKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWPQTLNGPSGHEPAALNLSASWL
DKMSTEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQ
GKEVTAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYAG
ARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSW
PEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFM
YARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=302, Percent_Identity=26.158940397351, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI61835204, Length=300, Percent_Identity=24.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI194473681, Length=300, Percent_Identity=24.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI194473668, Length=300, Percent_Identity=24.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI87081967, Length=435, Percent_Identity=99.0804597701149, Blast_Score=889, Evalue=0.0,
Organism=Escherichia coli, GI87082121, Length=249, Percent_Identity=22.0883534136546, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 48712; Mature: 48581

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFSMKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCC
QTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRL
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
QKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAW
HHCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECC
GAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHH
GARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLD
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEE
MEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHN
HHHHCCHHHHCCCCEEEEHHCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCC
PDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFMYARAMGWKNVSVYDGGWYE
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEE
WSSDPKNPVATGERGPDSSK
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGFSMKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAIDTRPSAFYNGWP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCCHHHCCCC
QTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALYGNDKDVDAVKTRL
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
QKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEVTAKPAGDWKVIEAAW
HHCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECC
GAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKHGIRHDTTVILYGRDVYA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHH
GARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKVKAEPDFGVKIPAQPQLMLD
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCEECCCCCHHEEE
MEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGEIAGARWGHAGSDSTHMEDFHN
HHHHCCHHHHCCCCEEEEHHCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHCC
PDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRASETFMYARAMGWKNVSVYDGGWYE
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCEEE
WSSDPKNPVATGERGPDSSK
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9097039 [H]