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The map label for this gene is insB [H]

Identifier: 82543775

GI number: 82543775

Start: 1259056

End: 1259532

Strand: Direct

Name: insB [H]

Synonym: SBO_1266

Alternate gene names: 82543775

Gene position: 1259056-1259532 (Clockwise)

Preceding gene: 82543774

Following gene: 82543782

Centisome position: 27.86

GC content: 52.41

Gene sequence:

>477_bases
TTGACGCTTACACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGTAACCTCGCGCATACAACCGGGCAGTGA
TGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGT
ATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTG
CTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGT
TATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGCCTGGGACGGAAGT
CACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
ATCCTGAGCGTACTGCCGGAATGGCCTTCCAACCGTGTTGACGAACTCCTGCCATGGAACGTAGCACTCACCAATAAATA
AGCGTCAATACGGTGCTCCG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCCGAAAATAGTAGTTACAAGAGAACGACTGAAACTAAAGACTGAGGTGTACTGGCAATAGCGGACA
CTACCATTTGTTCTTTTTTT

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTLTHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSL
LSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLASLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_158_residues
MTLTHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSL
LSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLASLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_157_residues
TLTHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLL
SAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLASLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=154, Percent_Identity=92.8571428571429, Blast_Score=299, Evalue=5e-83,
Organism=Escherichia coli, GI1788203, Length=154, Percent_Identity=92.8571428571429, Blast_Score=299, Evalue=5e-83,
Organism=Escherichia coli, GI1786203, Length=154, Percent_Identity=92.8571428571429, Blast_Score=299, Evalue=5e-83,
Organism=Escherichia coli, GI1787223, Length=154, Percent_Identity=90.9090909090909, Blast_Score=295, Evalue=1e-81,
Organism=Escherichia coli, GI1786470, Length=154, Percent_Identity=90.9090909090909, Blast_Score=294, Evalue=2e-81,
Organism=Escherichia coli, GI1786459, Length=154, Percent_Identity=90.9090909090909, Blast_Score=294, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 18483; Mature: 18351

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRT
CCCCCHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
VVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHN
HHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHCC
LNLRQHLASLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
CCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLTHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRT
CCCCHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
VVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHN
HHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHCC
LNLRQHLASLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
CCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]