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The map label for this gene is pphA [H]

Identifier: 82543762

GI number: 82543762

Start: 1248969

End: 1249628

Strand: Reverse

Name: pphA [H]

Synonym: SBO_1253

Alternate gene names: 82543762

Gene position: 1249628-1248969 (Counterclockwise)

Preceding gene: 82543763

Following gene: 82543756

Centisome position: 27.65

GC content: 47.88

Gene sequence:

>660_bases
TTGATGAAACAGCCTGCGCCCGTTTATCAGAGAATTGCGGGTCATCAATGGCGACATATCTGGCTTTCTGGCGATATACA
CGGTTGTCTTGAGCAGTTGCGCCGCAAATTATGGCATTGTCGTTTTGATCCGTGGCGAGATTTACTTATCTCAGTGGGAG
ACGTTATCGATCGTGGGCCGCAAAGTTTACGTTGTCTGCAGTTACTGGAACAACATTGGGTTTGTGCGGTAAGAGGCAAT
CATGAACAGATGGCGATGGATGCGCTGGCATCCCAGCAGATGTCTTTGTGGTTGATGAATGGCGGCGACTGGTTTATTGC
GCTGGCAGATAATCAACAGAAACAAGCGAAAACGGCGCTGGAAAAATGTCAGCATTTGCCCTTTATTCTTGAAGTACACA
GTCGCACCGGCAAGCATGTTATTGCTCATGCGGATTATCCAGATGATGTTTATGAATGGCAAAAGGACGTTGATTTGCAT
CAGGTCTTGTGGAGCCGCTCGCGATTAGGTGAACGCCAAAAAGGGCAGGGAATTACAGGTGCTGATCATTTCTGGTTTGG
TCATACACCGTTGCGACATCGCGTGGATATTGGCAACCTGCATTATATTGATACCGGTGCTGTCTTTGGGGGCGAACTGA
CTCTTGTGCAATTGCAATGA

Upstream 100 bases:

>100_bases
TGACTTTATAGTCAACCATAACACACACTCTACTGTCTGAGTCCAGCGTTTTTTAACATTCTTGTTAAGATTATGTGATC
TTTAGCACGGGAGGAAAATA

Downstream 100 bases:

>100_bases
TTAAAAATCACCGTACTCCTGTGCAGGTCGCCAGAAACCATCTATAAAATCCTCAATCGGAAAACAACCGCCCTGGCGGA
TCCGTTGATCGCTCATAGAA

Product: serine/threonine protein phosphatase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MMKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGN
HEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLH
QVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ

Sequences:

>Translated_219_residues
MMKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGN
HEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLH
QVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
>Mature_219_residues
MMKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGN
HEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLH
QVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ

Specific function: Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr an

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=218, Percent_Identity=100, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1789090, Length=217, Percent_Identity=43.778801843318, Blast_Score=188, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25405; Mature: 25405

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGP
CCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
QSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTAL
HHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCHHHHHHHH
EKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGITG
HHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCC
ADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
CCCEEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC
>Mature Secondary Structure
MMKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGP
CCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
QSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTAL
HHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCHHHHHHHH
EKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGITG
HHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCC
ADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
CCCEEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9097040; 9278503 [H]