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The map label for this gene is intB [H]

Identifier: 82543731

GI number: 82543731

Start: 1217187

End: 1218449

Strand: Direct

Name: intB [H]

Synonym: SBO_1221

Alternate gene names: 82543731

Gene position: 1217187-1218449 (Clockwise)

Preceding gene: 82543729

Following gene: 82543735

Centisome position: 26.93

GC content: 53.05

Gene sequence:

>1263_bases
ATGTCTTTAAACGATTCAAAAATCCGTAACTTAAAACCCTCTTCAAAACCCGTAAAACTGTCTGATTCGCACGGTCTGTA
TCTGCTGGTCAATCAGGGCGGTTCGCGTATCTGGTATCTCAAGTATCGCTTCAGTGGTAAAGAGTCCCGCGTCAGTCTGG
GCGCATATCCTCTGGTTTCACTTGCTGAAGCAAGACAACGACGTGACGATATCCGTAAGCTACTGACGCAGAATATTAAC
CCCGCCCACCAGCGCATGTCTGATAAAGCCGCAGCGTCACCGGAAAAGTATTTTAAAGCCGTGGCGCTTGCATGGCACAA
AACCAACAAAAAATGGTCGCCTGATTATGCCGCGCGCATCCTCGCCAGCATGGAAAATCATATCTTTCCGGCGATTGGTC
ATCTGCCTGTCACTACGCTGAAAACGCAGAATTTCACTGCGTTACTGCGGGTTATTGAGGATAAAGGTTTTCTGGAAGTC
GCGTCCCGAACCCGGCAGCAACTCTGCAACATCATGCGTTACGCCGTACAGCAGGGATTCACCGAAAACAACCTGGCGCA
GCATCTGGAAGGCGTCACCGCGCCGCCGGTTAAGAATCACTATCCCGCCCTGCCGCTGGAGCGGTTGCCCGAATTGTTTG
AGCGCATTGGCGACTATCAGCAGGGACGGCAGCTTACGAGGCTGGCGGTGGTGTTGACTCTCCACCTGTTTATCCGTTCC
AGCGAGCTGCGCTTTGCCCGCTGGGGTGAGATAGATTTCAGAAACAAAATCTGGACCATCCCCGCCACAAGGGAAGCGAT
TGAGAAAGTCCGTTTCTCTGGCCGTGGCGCAAAAATGCGTACTCCATATATAGTGCCGCTTTCTCGTCAGGCTATCGCCA
TTCTGAAGCAGATAAAGGAAATCTCCGGCCATCTGGAACTGGTGTTCCCCGGCGACCATAACCCGTATAAGCCGATGAGC
GAAAACACTGTCAACCGGGCGTTGCGTCTTATGGGCTATGATACCAAAACTGATGTCTGTGGGCATGGATTCCGGACAAT
GGCGTGTAGCGCATTAGTGGAAACTGAACTCTGGTCACGGGATACCGTGGAGCGGCAGATGAGTCATCAGGAGCGCAACA
GCGTTCGGGCAGCGTATATCCACCGGGCTGAACATCTCGACGCCCGTACAGCCATGATGCAGTGGTGGTCGGATTATCTG
GATGTAAGTCGCGAGGGGTACGTTGCGCCGTATATTTATGCGCGGCGGCATAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
GAAAATCTTTCCAGATGTGTATTCAGTTTTCCCTCCGCATCCGGTTAATTTGGTGGTCAGATTTGGGGGCAGGTTGGTTC
GATTCTGGAGTGACCCCCAA

Downstream 100 bases:

>100_bases
GACAGTGGCTATATAATTTGTTAAAAATGAAAAAGCAGATCCTGATTGAATCCGGATCTGCTTTTTACATTAGGCAATCG
CTGCCAGACAATCTGCACCA

Product: P4-type integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRFSGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNIN
PAHQRMSDKAAASPEKYFKAVALAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGDYQQGRQLTRLAVVLTLHLFIRS
SELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRGAKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMS
ENTVNRALRLMGYDTKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAMMQWWSDYL
DVSREGYVAPYIYARRHKAA

Sequences:

>Translated_420_residues
MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRFSGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNIN
PAHQRMSDKAAASPEKYFKAVALAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGDYQQGRQLTRLAVVLTLHLFIRS
SELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRGAKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMS
ENTVNRALRLMGYDTKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAMMQWWSDYL
DVSREGYVAPYIYARRHKAA
>Mature_419_residues
SLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRFSGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINP
AHQRMSDKAAASPEKYFKAVALAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEVA
SRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGDYQQGRQLTRLAVVLTLHLFIRSS
ELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRGAKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSE
NTVNRALRLMGYDTKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAMMQWWSDYLD
VSREGYVAPYIYARRHKAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=401, Percent_Identity=32.1695760598504, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788974, Length=411, Percent_Identity=31.8734793187348, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI145693166, Length=407, Percent_Identity=31.6953316953317, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 48298; Mature: 48167

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRFSGKESRVSLGAYPLVS
CCCCCCHHHCCCCCCCCEEEECCCCEEEEEECCCCEEEEEEEEECCCCCCEECCCCHHHH
LAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVALAWHKTNKKWSPDYAARI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEVASRTRQQLCNIMRYAVQQGF
HHHHHHCCCCHHCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
TENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGDYQQGRQLTRLAVVLTLHLFIRS
CCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
SELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRGAKMRTPYIVPLSRQAIAILKQIKE
CCCCEEECCCEECCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHH
ISGHLELVFPGDHNPYKPMSENTVNRALRLMGYDTKTDVCGHGFRTMACSALVETELWSR
HCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCH
DTVERQMSHQERNSVRAAYIHRAEHLDARTAMMQWWSDYLDVSREGYVAPYIYARRHKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
SLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRFSGKESRVSLGAYPLVS
CCCCCHHHCCCCCCCCEEEECCCCEEEEEECCCCEEEEEEEEECCCCCCEECCCCHHHH
LAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVALAWHKTNKKWSPDYAARI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEVASRTRQQLCNIMRYAVQQGF
HHHHHHCCCCHHCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
TENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGDYQQGRQLTRLAVVLTLHLFIRS
CCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
SELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRGAKMRTPYIVPLSRQAIAILKQIKE
CCCCEEECCCEECCCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHH
ISGHLELVFPGDHNPYKPMSENTVNRALRLMGYDTKTDVCGHGFRTMACSALVETELWSR
HCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCH
DTVERQMSHQERNSVRAAYIHRAEHLDARTAMMQWWSDYLDVSREGYVAPYIYARRHKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]