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The map label for this gene is yeiT [H]

Identifier: 82543533

GI number: 82543533

Start: 1015207

End: 1016445

Strand: Reverse

Name: yeiT [H]

Synonym: SBO_0995

Alternate gene names: 82543533

Gene position: 1016445-1015207 (Counterclockwise)

Preceding gene: 82543534

Following gene: 82543532

Centisome position: 22.49

GC content: 51.49

Gene sequence:

>1239_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGCCGTTACTGGCGATTAAAGAAGCCTCTCGCTGTTT
ATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTC
GTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAG
AAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGA
TTTTGAACAACAAACCGGGATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTC
CTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATCTATGAGAAAGAAGCACAACCCGGT
GGCTGGTTGCGTAACGGCATTCCGCAATTCCGGTTACCGCAATCAGTACTGGATGCAGAGATCGCCCGTATTGAAAAAAT
GGGCGTGACCATTAAGTGCAACAACGAAGTCGGTAACACACTCACCCTTGAGCAGCTGAAAGCAGAAAACCGCGCGGTAC
TGGTCACCGTGGGGTTATCAAGCGGTTCCGGGCTACCGCTGTTTGAGCATAGTGACGTTGAGATTGCCGTCGACTTCTTG
CAACGTGCACGACAGGCGCAAGGCGATATCAGCATTCCACAAAGCGCATTAATTATCGGCGGCGGTGATGTCACGATGGA
CGTAGCCAGCACGCTGAAAGTTCTCGGCTGTCAGGCGGTAACTTGCGTAGCGCGTGAAGAGTTAGATGAGTTTCCGGCAA
GCGAAAAAGAGTTTACCAGCGCCCGGGAGCTGGGCGTTTCGATCATCGATGGATTCACGCCAGTAGCCGTGGAAGGCAAT
AAAGTCACGTTTAAGCATGTACGGTTATCGGGCGAACTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCA
TGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGC
AAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCATGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAG
GCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAA

Upstream 100 bases:

>100_bases
GGATGGGCCGAATAACCCGCAGACGCAGCACATGCAACTTGAAGTATGACGAGTATATTTAGCGTTCGCCTTAATTACTC
ATAGCATTAAGGAAGATCAC

Downstream 100 bases:

>100_bases
CGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGC
TATGAGATGTGTGCCAAAGC

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGN
KVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAVKTGKEAAE
AIHHYLEGACSC

Sequences:

>Translated_412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
QRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGN
KVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAVKTGKEAAE
AIHHYLEGACSC
>Mature_411_residues
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPGG
WLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFLQ
RARQAQGDISIPQSALIIGGGDVTMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNK
VTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAVKTGKEAAEA
IHHYLEGACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=437, Percent_Identity=31.8077803203661, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=48.8888888888889, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=99.0291262135922, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=449, Percent_Identity=31.6258351893096, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=31.2217194570136, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI87082180, Length=449, Percent_Identity=28.9532293986637, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789243, Length=400, Percent_Identity=29.5, Blast_Score=138, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=32.8798185941043, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=449, Percent_Identity=27.1714922048998, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=24.9457700650759, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=31.1059907834101, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=31.1059907834101, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=28.1531531531532, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=28.1531531531532, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=28.47533632287, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=28.47533632287, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44393; Mature: 44262

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTS
HHHHHCCCCCCCCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEEECCCEEEHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
QRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLGCQAVTCVAREELDEFPASEKEFTS
HHHHHCCCCCCCCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEEECCCEEEHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]