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The map label for this gene is insB

Identifier: 82543383

GI number: 82543383

Start: 847904

End: 848113

Strand: Direct

Name: insB

Synonym: SBO_0831

Alternate gene names: 82543383

Gene position: 847904-848113 (Clockwise)

Preceding gene: 82543382

Following gene: 82543387

Centisome position: 18.76

GC content: 48.57

Gene sequence:

>210_bases
ATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCAT
TGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGC
TGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
CAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGAGTTCACAGATAAAACACTCTCCAG
GAAACCCGGGGCGGTTCAGG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCACCAGTGCGGTTAATAACAATGGAGTTACAGAACTTTTTGCCTTGCTGCATACAGAAGATGTCTG
TCGCTAATTTCGGTAAGTTA

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_69_residues
MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_68_residues
TDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family

Homologues:

Organism=Escherichia coli, GI1789853, Length=69, Percent_Identity=100, Blast_Score=142, Evalue=4e-36,
Organism=Escherichia coli, GI1788203, Length=69, Percent_Identity=100, Blast_Score=142, Evalue=4e-36,
Organism=Escherichia coli, GI1786203, Length=69, Percent_Identity=100, Blast_Score=142, Evalue=4e-36,
Organism=Escherichia coli, GI1787223, Length=69, Percent_Identity=98.5507246376812, Blast_Score=140, Evalue=2e-35,
Organism=Escherichia coli, GI1786470, Length=69, Percent_Identity=98.5507246376812, Blast_Score=139, Evalue=2e-35,
Organism=Escherichia coli, GI1786459, Length=69, Percent_Identity=98.5507246376812, Blast_Score=139, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): INSB1_ECOLI (P0CF25)

Other databases:

- EMBL:   J01729
- EMBL:   X52534
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JN0135
- RefSeq:   AP_000685.1
- RefSeq:   AP_002888.1
- RefSeq:   AP_004347.1
- RefSeq:   NP_414562.1
- RefSeq:   NP_416407.1
- RefSeq:   NP_417902.1
- ProteinModelPortal:   P0CF25
- EnsemblBacteria:   EBESCT00000005071
- EnsemblBacteria:   EBESCT00000015770
- GeneID:   944743
- GeneID:   945867
- GeneID:   947949
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0020
- KEGG:   eco:b0021
- EchoBASE:   EB4700
- EcoGene:   EG40002
- eggNOG:   COG1662
- GeneTree:   EBGT00070000031710
- HOGENOM:   HBG468514
- InterPro:   IPR005063

Pfam domain/function: PF03400 Transposase_27

EC number: NA

Molecular weight: Translated: 8296; Mature: 8165

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIG
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
HYLNIKHYQ
HHHCCCCCC
>Mature Secondary Structure 
TDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIG
CCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
HYLNIKHYQ
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503