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The map label for this gene is cmr [H]

Identifier: 82543292

GI number: 82543292

Start: 755675

End: 756883

Strand: Direct

Name: cmr [H]

Synonym: SBO_0738

Alternate gene names: 82543292

Gene position: 755675-756883 (Clockwise)

Preceding gene: 82543288

Following gene: 82543296

Centisome position: 16.72

GC content: 52.36

Gene sequence:

>1209_bases
GTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAGATAT
GATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCAATGACCGCATATC
TGGCGGGCGGGATGTTTTTACAATGGCTCCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCGGTGATGCTGGCGGGG
GTGGTGTGGTTTATCATCACCTGTCTGGCAATATTACTGGCGCAAAACATTGAACAATTCACTCTGTTGCGCTTCTTGCA
GGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCTTTCGAAGAGGCGGTTTGTATCAAGA
TCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTG
CCCTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCTATGCCTGAAAC
CGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTG
TGGCGGGGGCGCTGGCGCTGGGATTCGTTAGCCTGCCATTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACC
GGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAATTGCGGGTAACTTGCTGTT
AGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATAATTATGGGCGGCTGGCCGATTATGATTGGTCTGTTGGTCG
CTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACTGCCGGGTTAAGTATTTATGCTTTCGGTATTGGT
CTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGTTTCTGCGGCGATGGGAAT
GCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACAGTTTAATC
TCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTCTCAC
GAAGGGTAA

Upstream 100 bases:

>100_bases
TTTCAGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCAT
GCAAAATAAATTAGCTTCCG

Downstream 100 bases:

>100_bases
AAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATC
CCTTCACCTACGCTGGCAAG

Product: proton motive force efflux pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPGLDVRRYFSLSVWCFTNFQPISADMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAG
VVWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVL
PWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIIT
GEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIG
LANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLKDKQMGNSH
EG

Sequences:

>Translated_402_residues
MPGLDVRRYFSLSVWCFTNFQPISADMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAG
VVWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVL
PWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIIT
GEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIG
LANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLKDKQMGNSH
EG
>Mature_401_residues
PGLDVRRYFSLSVWCFTNFQPISADMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGV
VWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLP
WEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIITG
EQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGL
ANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLKDKQMGNSHE
G

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=391, Percent_Identity=96.6751918158568, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI1790794, Length=387, Percent_Identity=39.5348837209302, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI48994889, Length=216, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788509, Length=284, Percent_Identity=23.2394366197183, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790146, Length=147, Percent_Identity=27.891156462585, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43598; Mature: 43466

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLDVRRYFSLSVWCFTNFQPISADMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFL
CCCCCHHHHHEEEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAALAAISFFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHH
QRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIIT
HHHCHHHHHHHHCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
GEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVI
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEIS
HCCCCEEEECCCHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCEEE
KHAWLNGGNGQFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PGLDVRRYFSLSVWCFTNFQPISADMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFL
CCCCHHHHHEEEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAALAAISFFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHH
QRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIIT
HHHCHHHHHHHHCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
GEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVI
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEIS
HCCCCEEEECCCHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCEEE
KHAWLNGGNGQFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]