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The map label for this gene is brnQ

Identifier: 82542890

GI number: 82542890

Start: 308657

End: 309976

Strand: Direct

Name: brnQ

Synonym: SBO_0295

Alternate gene names: 82542890

Gene position: 308657-309976 (Clockwise)

Preceding gene: 82542889

Following gene: 82542891

Centisome position: 6.83

GC content: 55.76

Gene sequence:

>1320_bases
ATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACAT
TATTTTCCCTCCAATGGTCGGCTTGCAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTACTGCCGTTG
GCCTGCCGGTATTAACGGTAGTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTC
GCTGGCGTGCTGCTGGCAACGGTTTGTTACTTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTTCCTT
TGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTA
TTCTGGTTTCGCTCTATCCGGGCAAGCTGCTGGATACCGTGGGCAACTTCCTTGCGCCGCTGAAAATTATCGCGCTGGTC
ATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTC
TAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGC
GTTCTCGTGGCGTTACCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACT
CTGCTGTACCTGGCGCTGTTCCGTCTGGGTTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCT
GCATGCTTACGTTCAGCACACATTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAA
CGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATC
CTCGGCGGCTTCTCGATGGTGGTGTCTAACCTCGGCTTAAGCCAGCTGATCCAGATCTCCGTACCGGTGCTGACCGCTAT
TTATCCGCCGTGTATCGCACTGGTTGTATTAAGTTTTACACGCTCATGGTGGCATAATTCGTCCCGCGTGATTGCCCCGC
CGATGTTTATCAGCCTGCTTTTTGGTATTCTCGACGGGATCAAAGCATCTGCATTCAGCGATATCTTACCGTCCTGGGCG
CAGCGTTTACCGCTGGCCGAACAAGGCCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGA
TCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAA

Upstream 100 bases:

>100_bases
TATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCAC
ATCCACAGGCAGTATGATTT

Downstream 100 bases:

>100_bases
ATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGA
AAGTAAGAACAAGCTAAAGC

Product: branched chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKV
AGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVTEARLLTRYTVWAGLMAGVGLT
LLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFI
LGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAFSDILPSWA
QRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH

Sequences:

>Translated_439_residues
MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKV
AGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVTEARLLTRYTVWAGLMAGVGLT
LLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFI
LGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAFSDILPSWA
QRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH
>Mature_438_residues
THQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVA
GVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALVI
LSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVTEARLLTRYTVWAGLMAGVGLTL
LYLALFRLGSDSASLVDQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFIL
GGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAFSDILPSWAQ
RLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH

Specific function: Component of the LIV-II transport system for branched- chain amino acids. This LIV-II transport system may be H(+)- coupled

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family

Homologues:

Organism=Escherichia coli, GI1786601, Length=439, Percent_Identity=100, Blast_Score=863, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BRNQ_ECO57 (P0ADA0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90685
- PIR:   G85535
- RefSeq:   NP_286139.1
- RefSeq:   NP_308478.1
- ProteinModelPortal:   P0ADA0
- EnsemblBacteria:   EBESCT00000027306
- EnsemblBacteria:   EBESCT00000058762
- GeneID:   914553
- GeneID:   957352
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0499
- KEGG:   ecs:ECs0451
- GeneTree:   EBGT00050000008973
- HOGENOM:   HBG533860
- OMA:   ASSHYLF
- ProtClustDB:   PRK15433
- BioCyc:   ECOL83334:ECS0451-MONOMER
- InterPro:   IPR004685
- TIGRFAMs:   TIGR00796

Pfam domain/function: PF05525 Branch_AA_trans

EC number: NA

Molecular weight: Translated: 46209; Mature: 46078

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17681f3c)-; HASH(0x177db274)-; HASH(0x177a0f90)-; HASH(0x17752944)-; HASH(0x1770400c)-; HASH(0x17727fd4)-; HASH(0x177705ac)-; HASH(0x177d8224)-; HASH(0x176b48c8)-; HASH(0x174994dc)-; HASH(0x17726644)-; HASH(0x1768e978)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTV
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDSAL
HHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEECCCCCHH
PLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALVILSVAAIVWPAGSISTATEA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
YQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVTEARLLTRYTVWAGLMAGVGLT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTC
HHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWWHNSSRVIAPPMFISLLFGILDGIKASAFSDILPSWAQRLPLAEQGLAWLMPTVVMV
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAIIWDRAAGRQVTSSAH
HHHHHHHCCCCCCCCCCCH
>Mature Secondary Structure 
THQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTV
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDSAL
HHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEECCCCCHH
PLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALVILSVAAIVWPAGSISTATEA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
YQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVTEARLLTRYTVWAGLMAGVGLT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTC
HHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWWHNSSRVIAPPMFISLLFGILDGIKASAFSDILPSWAQRLPLAEQGLAWLMPTVVMV
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAIIWDRAAGRQVTSSAH
HHHHHHHCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796