BacMap

Definition	Shigella boydii Sb227, complete genome.
Accession	NC_007613
Length	4,519,823

Click here to switch to the map view.

The map label for this gene is dkgB [H]

Identifier: 82542800

GI number: 82542800

Start: 215184

End: 215924

Strand: Direct

Name: dkgB [H]

Synonym: SBO_0196

Alternate gene names: 82542800

Gene position: 215184-215924 (Clockwise)

Preceding gene: 82542799

Following gene: 82542802

Centisome position: 4.76

GC content: 47.1

Gene sequence:

>741_bases
GTGAAAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGC
GATTGCAGAAAGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAAT
TGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCA
AACGATGAAGTCTCTGTTGAAGGGGTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTAT
TTCCAACTTCACGATCCCATTGATGGAAAAGGCGATTGCTGCTGTTGGCGCTGAAAACATCGCTACTAACCAGATTGAAC
TCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTG
GCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCT
GGCGTGGGCTATGGGGGAAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTAAAAACCTGGAAAGTAATCTTAAGGCAC
AAAATTTACAGCTTGATGCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGAAGGT
CTGGCTCCTGAATGGGATTAA

Upstream 100 bases:

>100_bases
ACAGTCTGAAGCCCATAATCACCTCAGTTAACGAAAATAGCATTAAAAGAGGCATATTATGGCTATCCCTGCATTTGGTT
TAGGTTGAAAGACTACATCT

Downstream 100 bases:

>100_bases
GCCTCTCTGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTAC
CGCACGGTCGCTGTAATAGA

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSP
NDEVSVEGVMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMTL
AYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEG
LAPEWD

Sequences:

>Translated_246_residues
MKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSP
NDEVSVEGVMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMTL
AYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEG
LAPEWD
>Mature_246_residues
MKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSP
NDEVSVEGVMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMTL
AYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEG
LAPEWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=252, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI310109920, Length=269, Percent_Identity=31.5985130111524, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI45446745, Length=270, Percent_Identity=31.1111111111111, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI4503285, Length=270, Percent_Identity=31.1111111111111, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI5453543, Length=269, Percent_Identity=31.2267657992565, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI24497583, Length=274, Percent_Identity=31.7518248175182, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI24497585, Length=268, Percent_Identity=30.5970149253731, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI5174695, Length=269, Percent_Identity=30.8550185873606, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI24497577, Length=270, Percent_Identity=29.2592592592593, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI5174391, Length=270, Percent_Identity=29.2592592592593, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI223468663, Length=266, Percent_Identity=30.0751879699248, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI291291012, Length=266, Percent_Identity=29.6992481203008, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI93277124, Length=280, Percent_Identity=29.2857142857143, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI300116273, Length=245, Percent_Identity=30.2040816326531, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4502049, Length=269, Percent_Identity=33.0855018587361, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI300116271, Length=254, Percent_Identity=30.3149606299213, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI310109926, Length=81, Percent_Identity=43.2098765432099, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI207028673, Length=82, Percent_Identity=41.4634146341463, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786400, Length=244, Percent_Identity=99.1803278688525, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI87082198, Length=237, Percent_Identity=39.2405063291139, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1788081, Length=243, Percent_Identity=29.2181069958848, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI48994888, Length=246, Percent_Identity=29.6747967479675, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787674, Length=240, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17550248, Length=252, Percent_Identity=35.7142857142857, Blast_Score=140, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71998625, Length=237, Percent_Identity=30.8016877637131, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17537075, Length=263, Percent_Identity=34.6007604562738, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17537077, Length=261, Percent_Identity=34.4827586206897, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17552492, Length=240, Percent_Identity=32.5, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17561298, Length=254, Percent_Identity=31.1023622047244, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17561300, Length=258, Percent_Identity=31.0077519379845, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17566692, Length=250, Percent_Identity=31.2, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538386, Length=259, Percent_Identity=30.1158301158301, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562292, Length=248, Percent_Identity=29.4354838709677, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17537079, Length=275, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17564128, Length=272, Percent_Identity=25.7352941176471, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324694, Length=252, Percent_Identity=35.3174603174603, Blast_Score=145, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320576, Length=256, Percent_Identity=35.546875, Blast_Score=142, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321896, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322556, Length=235, Percent_Identity=27.2340425531915, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319625, Length=254, Percent_Identity=30.7086614173228, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320079, Length=240, Percent_Identity=30, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24644950, Length=272, Percent_Identity=34.1911764705882, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24657054, Length=265, Percent_Identity=32.0754716981132, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI221378297, Length=292, Percent_Identity=31.8493150684932, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356425, Length=266, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24662789, Length=274, Percent_Identity=30.6569343065693, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI20129731, Length=266, Percent_Identity=30.8270676691729, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24663317, Length=266, Percent_Identity=28.9473684210526, Blast_Score=113, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24662785, Length=271, Percent_Identity=33.5793357933579, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24662781, Length=271, Percent_Identity=33.5793357933579, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45553081, Length=262, Percent_Identity=27.8625954198473, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=27.027027027027, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 27144; Mature: 27144

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKE
CCCHHHHCCHHHCHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCCCCHHHHH
SLQKLRTDYVDLTLIHWPSPNDEVSVEGVMQALLEAKKQGLTREIGISNFTIPLMEKAIA
HHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
AVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNA
HHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
TPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEG
CHHHEEEEEECCCCCEECCCCHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCEECCCC
LAPEWD
CCCCCC
>Mature Secondary Structure
MKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKE
CCCHHHHCCHHHCHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCCCCHHHHH
SLQKLRTDYVDLTLIHWPSPNDEVSVEGVMQALLEAKKQGLTREIGISNFTIPLMEKAIA
HHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
AVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNA
HHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
TPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEG
CHHHEEEEEECCCCCEECCCCHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCEECCCC
LAPEWD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]