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The map label for this gene is hemL [H]

Identifier: 82542754

GI number: 82542754

Start: 159632

End: 160912

Strand: Reverse

Name: hemL [H]

Synonym: SBO_0143

Alternate gene names: 82542754

Gene position: 160912-159632 (Counterclockwise)

Preceding gene: 82542757

Following gene: 82542747

Centisome position: 3.56

GC content: 55.89

Gene sequence:

>1281_bases
ATGAGTAAGTCTGAAAATCTTTACAGCGCAGCGCGCGAGCTGCTCCCTGGCGGTGTGAACTCCCCTGTTCGCGCCTTTAC
TGGCGTGGGCGGCACTCCACTGTTTATCGAAAAAGCGGACGGCGCTTATCTGTACGATGTTGATGGCAAAGCCTATATCG
ATTATGTCGGTTCCTGGGGGCCGATGGTGCTGGGCCATAACCATCCGGCTATCCGCAATGCCGTGATTGAAGCCGCCGAG
CGTGGTTTAAGCTTTGGTGCACCGACCGAAATGGAAGTGAAAATGGCGCAACTGGTGACCGAACTGGTCCCGACCATGGA
TATGGTGCGCATGGTGAACTCCGGCACTGAAGCGACCATGAGCGCCATCCGCCTGGCCCGTGGTTTTACCGGTCGCGACA
AAATCATTAAATTTGAAGGTTGTTACCACGGTCACGCTGACTGCCTGCTGGTGAAAGCCGGTTCTGGCGCACTCACGTTA
GGCCAGCCAAACTCGCCGGGTGTTCCGGCAGATTTCGCCAAACATACCTTAACCTGTACTTATAACGATCTGGCTTCTGT
ACGCGCCGCGTTTGAGCAATACCCGCAAGAGATTGCCTGTATTATCGTCGAGCCGGTGGCAGGCAATATGAACTGTGTTC
CGCCGCAGCCAGAGTTCCTGCCAGGTCTGCGTGCGCTGTGCGACGAATTTGGCGCGTTGCTGATCATCGATGAAGTGATG
ACCGGCTTCCGCGTGGCACTGGCTGGCGCACAGGATTATTACGGCGTAGTGCCAGATTTAACCTGCCTCGGCAAAATCAT
CGGCGGTGGAATGCCGGTAGGCGCATTCGGTGGTCGTCGTGATGTAATGGATGCGCTGGCCCCGACGGGTCCGGTCTATC
AGGCGGGTACGCTTTCCGGTAACCCGATTGCGATGGCAGCGGGTTTCGCCTGTCTGAATGAAGTCGCGCAGCCGGGCGTT
CACGAAACGCTGGATGAGCTGACAACACGTCTGGCAGAAGGTCTGCTGGAAGCGGCAGAAGAAGCCGGAACTCCGCTGGT
CGTTAACCACGTTGGCGGCATGTTCGGTATTTTCTTTACCGACGCCGAGTCCGTGACGTGCTATCAGGATGTGATGGCCT
GTGACGTGGAACGCTTTAAGCGTTTCTTCCATATGATGCTGGACGAAGGTGTTTACCTGGCACCGTCAGCGTTTGAAGCG
GGCTTTATGTCTGTGGCGCACAGCATGGAAGATATCAATAACACCATCGATGCTGCACGTCGGGTATTTGCGAAGTTGTG
A

Upstream 100 bases:

>100_bases
TGCACCAGTACAAGCAGCCTGATGTTTGACGAGTATTTAACTTGTTATGAATAACATAGAATAGCAGCCATTCACTTTTT
CTACGAATCAGGAACCCTCC

Downstream 100 bases:

>100_bases
TAATTTTGCCTCGCCCACCGGGCGAGGCACTCAATTAACGGCTCTGCTTTCTCAACAAATAGATAAAATATGGCGCGCCG
ATAAAGGTCGACAGCAGCCC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGV
HETLDELTTRLAEGLLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL

Sequences:

>Translated_426_residues
MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAE
RGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGV
HETLDELTTRLAEGLLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEA
GFMSVAHSMEDINNTIDAARRVFAKL
>Mature_425_residues
SKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAER
GLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLG
QPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPEFLPGLRALCDEFGALLIIDEVMT
GFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVH
ETLDELTTRLAEGLLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAG
FMSVAHSMEDINNTIDAARRVFAKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=322, Percent_Identity=29.5031055900621, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI13994255, Length=349, Percent_Identity=26.647564469914, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI284507298, Length=237, Percent_Identity=29.957805907173, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=99.0610328638498, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=447, Percent_Identity=30.2013422818792, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787560, Length=346, Percent_Identity=32.0809248554913, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788044, Length=312, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789759, Length=317, Percent_Identity=31.8611987381703, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693181, Length=334, Percent_Identity=29.6407185628743, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786991, Length=292, Percent_Identity=27.3972602739726, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=299, Percent_Identity=29.0969899665552, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=360, Percent_Identity=28.6111111111111, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32564660, Length=406, Percent_Identity=24.384236453202, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=142, Percent_Identity=34.5070422535211, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=340, Percent_Identity=29.1176470588235, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324432, Length=323, Percent_Identity=27.2445820433437, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357415, Length=301, Percent_Identity=27.2425249169435, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356575, Length=321, Percent_Identity=28.0373831775701, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45344; Mature: 45212

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHEECCCHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGVHETLDELTTRLAEGLLEAAEEAGTPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC
>Mature Secondary Structure 
SKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCC
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
HHHHHHEECCCHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLNEVAQPGVHETLDELTTRLAEGLLEAAEEAGTPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFAKL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA