| Definition | Shigella boydii Sb227, complete genome. |
|---|---|
| Accession | NC_007613 |
| Length | 4,519,823 |
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The map label for this gene is mrcB [H]
Identifier: 82542749
GI number: 82542749
Start: 150949
End: 153471
Strand: Direct
Name: mrcB [H]
Synonym: SBO_0138
Alternate gene names: 82542749
Gene position: 150949-153471 (Clockwise)
Preceding gene: 82542748
Following gene: 82542750
Centisome position: 3.34
GC content: 55.25
Gene sequence:
>2523_bases ATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCG TTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAG GCAAAGGGCGTAAGCCTCGTGGCAAACGCGGCTGGCTATGGCTACTGCTAAAACTGGCTATCGTTTTTGCCGTGCTGATC GCCATTTACGGCGTTTATCTCGATCAAAAAATTCGTAGCCGTATTGATGGCAAGGTCTGGCAACTGCCTGCAGCAGTTTA TGGCCGAATGGTCAATCTTGAGCCAGATATGACCATCAGCAAGAACGAGATGGTGAAGCTGCTGGAGGCGACTCAGTATC GTCAGGTGTCGAAAATGACCCGTCCTGGCGAATTTACCGTGCAGGCCAACAGCATTGAGATGATCCGCCGTCCGTTTGAT TTCCCGGACAGTAAAGAAGGGCAGGTGCGTGCGCATCTGACCTTTGATGGTGATCATCTGGCGACCATCGTCAATATGGA GAACAACCGTCAGTTCGGCTTTTTCCGTCTTGATCCGCGTCTGATCACCATGATCTCCTCGCCAAACGGTGAGCAACGTC TGTTTGTGCCGCGCAGTGGTTTCCCTGATTTGCTGGTGGATACTTTGCTGGCGACAGAAGACCGCCATTTCTACGAGCAT GATGGGATCAGCCTCTACTCAATTGGGCGTGCGGTGCTGGCAAACCTGACCGCAGGTCGCACGGTGCAGGGTGCGAGTAC GCTGACGCAACAGCTGGTGAAAAACCTGTTCCTCTCCAGCGAGCGCTCTTACTGGCGTAAAGCGAACGAAGCCTACATGG CGCTGATCATGGACGCGCGTTACAGCAAAGACCGTATTCTTGAGCTGTATATGAACGAGGTGTATCTCGGTCAGAGCGGC GACAACGAAATCCGCGGCTTCCCGCTGGCAAGCTTGTATTACTTTGGTCGCCCGGTAGAAGAGCTAAGCCTCGACCAGCA GGCGCTGTTAGTCGGTATGGTGAAAGGGGCGTCCATCTACAACCCGTGGCGTAACCCGAAACTGGCGCTGGAGCGACGTA ATCTGGTGCTGCGTCTGCTGCAACAGCAACAGATTATTGATCAAGAACTCTATGACATGTTGAGTGCCCGTCCGCTGGGG GTTCAGCCGCGCGGTGGGGTGATCTCTCCTCAGCCAGCCTTTATGCAACTGGTGCGTCAGGAGCTGCAGGCAAAACTGGG CGATAAGGTAAAAGATCTCTCCGGCGTGAAGATCTTCACTACCTTTGACTCGGTGGCCCAGGACGCGGCAGAAAAAGCCG CCGTGGAAGGCATTCCGGCACTGAAGAAACAGCGTAAGTTGAGCGATCTTGAAACTGCGATTGTGGTCGTCGACCGCTTT AGTGGTGAAGTTCGTGCGATGGTGGGAGGTTCTGAGCCGCAGTTTGCGGGCTACAACCGTGCGATGCAGGCGCGTCGTTC GATTGGTTCCCTTGCAAAACCAGCGACTTATCTGACGGCTTTAAGCCAGCCGAAAATCTATCGCCTGAACACGTGGATTG CCGATGCGCCAATCGCGCTGCGTCAGCCGAATGGCCTGGTCTGGTCACCGCAGAACGATGACCGTCGTTATAGCGAAAGC GGCAGAGTGATGCTGGTGGATGCGTTGACCCGTTCGATGAACGTGCCGACGGTAAATCTGGGGATGGCGCTGGGGCTGCC TGCAGTTACGGATACCTGGATCAAACTGGGCGTACCGAAAGATCAGCTGCATCCGGTTCCGGCGATGCTGCTGGGGGCGT TGAACTTAACGCCAATCGAAGTGGCGCAGGCGTTCCAGACCATCGCCAGCGGTGGTAACCGTGCACCGCTTTCTGCACTG CGTTCGGTAATCGCGGAAGATGGCAAGGTGCTGTATCAGAGCTTCCCGCAGGCAGAACGCGCTGTTCCGGCGCAGGCGGC GTATCTGACACTATGGACCATGCAGCAGGTGGTACAACGTGGTACGGGTCGTCAGCTTGGGGCGAAATACCCGAACCTGC ATCTGGCAGGGAAAACAGGGACTACCAACAATAACGTAGATACTTGGTTTGCAGGCATTGACGGCAGCACGGTGACCATC ACCTGGGCAGGCCGTGATAACAACCAGCCGACCAAACTGTATGGAGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCT GGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACT TTGTTTGCAGCGGTGGCATGCGTGTCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAGCAGAGCGAGATGCAG CAGCAGCCGTCAGGCAATCCGTTTGATCAGTCTTCTCAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGA CGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAA
Upstream 100 bases:
>100_bases GGGACCGATCGAACACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCT GAATATTGCGGAGAAAAAGC
Downstream 100 bases:
>100_bases CATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTC ATCTGGTTGTTTATTAACCC
Product: penicillin-binding protein 1b
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 840; Mature: 839
Protein sequence:
>840_residues MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLI AIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSG DNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLG VQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAIVVVDRF SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSES GRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSAL RSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI TWAGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ QQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN
Sequences:
>Translated_840_residues MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLI AIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSG DNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLG VQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAIVVVDRF SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSES GRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSAL RSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI TWAGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ QQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN >Mature_839_residues AGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIA IYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF PDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEHD GISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGD NEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGV QPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAIVVVDRFS GEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESG RVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALR SVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTIT WAGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQQ QPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=844, Percent_Identity=98.5781990521327, Blast_Score=1554, Evalue=0.0, Organism=Escherichia coli, GI87082258, Length=251, Percent_Identity=34.6613545816733, Blast_Score=151, Evalue=2e-37, Organism=Escherichia coli, GI1788867, Length=515, Percent_Identity=26.9902912621359, Blast_Score=123, Evalue=4e-29, Organism=Escherichia coli, GI1789601, Length=127, Percent_Identity=37.007874015748, Blast_Score=78, Evalue=2e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 93722; Mature: 93591
Theoretical pI: Translated: 9.39; Mature: 9.39
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPR CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC GKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTIS CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCEEEECCCCCEEC KNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKEGQVRAHLTFDGDHL HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECCCEE ATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH EEEEEECCCCEEEEEEECCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHC DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDAR CCEEHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHCHHHEEEEECCC YSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIY CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCC NPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRGGVISPQPAFMQLVRQ CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH ELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAIVVVDRF HHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECC SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIAL CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECEEECCCEEE RQPNGLVWSPQNDDRRYSESGRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPK ECCCCCEECCCCCCCHHCCCCCEEEEEHHHHCCCCCEEECHHHHCCCHHHHHHEEECCCH DQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAER HHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHH AVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEE TWAGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGM EECCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEECCCC RVLPVWTSDPQSLCQQSEMQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN EEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHCCCCCHHHHHHHHCCCC >Mature Secondary Structure AGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGKGRKPR CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC GKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTIS CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCEEEECCCCCEEC KNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKEGQVRAHLTFDGDHL HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECCCEE ATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEH EEEEEECCCCEEEEEEECCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHC DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDAR CCEEHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHCHHHEEEEECCC YSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIY CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCC NPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRGGVISPQPAFMQLVRQ CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH ELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAIVVVDRF HHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECC SGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIAL CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECEEECCCEEE RQPNGLVWSPQNDDRRYSESGRVMLVDALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPK ECCCCCEECCCCCCCHHCCCCCEEEEEHHHHCCCCCEEECHHHHCCCHHHHHHEEECCCH DQLHPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAER HHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHH AVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTI CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEE TWAGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGM EECCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEECCCC RVLPVWTSDPQSLCQQSEMQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN EEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHCCCCCHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 3882429; 8202364; 9278503; 3920658; 8645198; 9244263; 3330753; 1885547; 10037771; 10564478; 9841666 [H]