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The map label for this gene is yaaF [H]

Identifier: 82542646

GI number: 82542646

Start: 27329

End: 28243

Strand: Direct

Name: yaaF [H]

Synonym: SBO_0029

Alternate gene names: 82542646

Gene position: 27329-28243 (Clockwise)

Preceding gene: 82542645

Following gene: 82542647

Centisome position: 0.6

GC content: 56.07

Gene sequence:

>915_bases
ATGCATTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGA
ACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACCACCCGCAACGCCCTGCAACTGCTGC
ATTTCTGGAATGCGGAGATTCCGCTCGCCCAGGGAGCCGCTGTGCCGCTGGTACGCGCACCGCGTGATGCGGCATCTGTG
CACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCAGCGTTTCTGGCGAT
TCGGGATGCCCTGATGCGTGCACCAGAGCCCGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTT
CACAATGCCCGGAATGTAAACCGTATATTCGTCGTCTGGTGATCATGGGCGGTTCTGCTGGTCGCGGCAACTGCACACCA
AACGCCGAGTTTAATATCGCTGCCGATCCAGAAGCGGCTGCCTGTGTCTTCCGTAGCGGCATCGAAATCGTCATGAGCGG
TCTGGATGTCACCAATCAGGCAATATTAACCCCTGACTATCTCGCTACTTTGCCGGAGTTGAATCGCACCGGAAAAATGC
TCCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGTGGACTGCGTATGCACGATCTTTGCGCTATTGCCTGG
CTGGTGCGCCCGGAATTGTTCACTCTCAAACCGTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGAC
GGTGGTTGATATCGACGGTTGCCTGGGCAAACCAGCCAATGTAAAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGC
AGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAA

Upstream 100 bases:

>100_bases
AAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCG
GCATTTTTTATGGAGAAAAC

Downstream 100 bases:

>100_bases
CCTGTCACATGTTATCGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTC
TAATTATCAGCGTTTTTGGC

Product: ribonucleoside hydrolase RihC

Products: uracil; ribose; xanthine; hypoxanthine; adenine; cytosine; guanine [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVLALAS

Sequences:

>Translated_304_residues
MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVLALAS
>Mature_304_residues
MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVLALAS

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=304, Percent_Identity=98.0263157894737, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=45.3074433656958, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1788486, Length=302, Percent_Identity=39.7350993377483, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17565698, Length=187, Percent_Identity=33.6898395721925, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=32.1052631578947, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=342, Percent_Identity=29.2397660818713, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24641839, Length=196, Percent_Identity=32.1428571428571, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583915, Length=188, Percent_Identity=31.9148936170213, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641837, Length=341, Percent_Identity=27.5659824046921, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 32525; Mature: 32525

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEI
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHC
IEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
HHHHHCCCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVL
HHCCCCEEECCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALAS
HHCC
>Mature Secondary Structure
MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEI
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHC
IEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
HHHHHCCCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVKVALDLDVKGFQQWVAEVL
HHCCCCEEECCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALAS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; xanthosine; inosine; adenosine; cytidine; guanosine [C]

Specific reaction: uridine + H2O = uracil + ribose xanthosine + H2O = xanthine + ribose inosine + H2O = hypoxanthine + ribose adenosine + H2O = adenine + ribose cytidine + H2O = cytosine + ribose guanosine + H2O = guanine + ribose [C]

General reaction: Nonspecific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA