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The map label for this gene is iclR [H]

Identifier: 161984803

GI number: 161984803

Start: 4079444

End: 4080268

Strand: Reverse

Name: iclR [H]

Synonym: SBO_4039

Alternate gene names: 161984803

Gene position: 4080268-4079444 (Counterclockwise)

Preceding gene: 82546357

Following gene: 82546349

Centisome position: 90.27

GC content: 53.58

Gene sequence:

>825_bases
ATGGTCGCACCCATTCCCGCGAAACGCGGCAGAAAACCCGCCGTTGCCACCGCACCAGCGACTGGACAGGTTCAGTCTTT
AACGCGTGGCCTGAAATTACTGGAGTGGATTGCCGAATCCAATGGCAGTGTGGCACTCACGGAACTGGCGCAACAAGCCG
GGTTACCCAATTCCACGACCCACCGCCTGCTAACCACAATGCAACAGCAGGGTTTCGTGCGTCAGGTTGGCGAACTGGGA
CACTGGGCAATCGGCGCACATGCCTTTATGGTCGGCAGCAGCTTTCTCCAGAGTCGTAATTTGTTAGCAATTGTTCACCC
TATCCTGCGCAATCTAATGGAAGAGTCTGGCGAAACGGTCAATATGGAGGTGCTTGATCAAAGCGATCACGAAGCGATTA
TTATCGACCAGGTACAGTGTACGCATCTGATGCGAATGTCCGCGCCTATCGGCGGTAAATTGCCGATGCACGCTTCCGGT
GCAGGTAAAGCCTTTTTAGCCCAACTGAGCGAAGAACAGGTGACGAAGCTGCTGCACCGCAAAGGGTTACATGCCTATAC
CCACGCAACGCTGGTGTCTCCTGTGCATTTAAAAGAAGATCTCGCCCAAACGCGCAAACGGGGTTATTCATTTGACGATG
AGGAACATGCGCTGGGGCTACGTTGCCTTGCAGCGTGTATTTTCGATGAGCACCGTGAACCGTTTGCCGCAATTTCTATT
TCCGGACCGATTTCACGTATTACCGATGACCGCGTGACCGAGTTTGGCGCAATGGTGATTAAAGCGGCGAAGGAAGTGAC
ACTGGCGTACGGTGGAATGCGCTGA

Upstream 100 bases:

>100_bases
ATTTGTTCAACATTAACTCATCGGATCAGTTCAGCAACTATTGCATTAGCTAACAATAAAAATGAAAATGATTCCCACGA
TGCAGAAAAAGGAGACTGTC

Downstream 100 bases:

>100_bases
CTTTTTCTGGCGGGCAGAGGCAATATTCTGCCCATCATACCTGAGGTAATGACTCCAACTTACTGATAGTGTTTTATGTT
CAGATAATGCCCGATGACCT

Product: transcriptional repressor IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR

Sequences:

>Translated_274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
>Mature_274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=274, Percent_Identity=99.6350364963504, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1786715, Length=264, Percent_Identity=42.0454545454545, Blast_Score=220, Evalue=9e-59,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=29.8387096774194, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=30.241935483871, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789999, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790752, Length=249, Percent_Identity=26.1044176706827, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788581, Length=251, Percent_Identity=24.7011952191235, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29798; Mature: 29798

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
HRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
NMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHR
EEEEECCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
HRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
NMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHR
EEEEECCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]